JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB231437

Anti-MSH2 antibody [SP46]

  • 20ul selling size
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

Be the first to review this product! Submit a review

|

(1 Publication)

Knockout Tested Rabbit Recombinant Monoclonal MSH2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2, MSH2

13 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)

Formalin-fixed, paraffin-embedded human placenta tissue stained for MSH2 using ab231437 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for MSH2 using ab231437 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)

Formalin-fixed, paraffin-embedded human endometrium tissue stained for MSH2 using ab231437 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)

Formalin-fixed, paraffin-embedded human prostate tissue stained for MSH2 using ab231437 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)

Formalin-fixed, paraffin-embedded human lung tissue stained for MSH2 using ab231437 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)

Formalin-fixed, paraffin-embedded human thyroid tissue stained for MSH2 using ab231437 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for MSH2 using ab231437 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)

Formalin-fixed, paraffin-embedded human cervix tissue stained for MSH2 using ab231437 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)

Formalin-fixed, paraffin-embedded human skin tissue stained for MSH2 using ab231437 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] (AB231437)

Formalin-fixed, paraffin-embedded human colon tissue stained for MSH2 using ab231437 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-MSH2 antibody [SP46] (AB231437)
  • WB

Lab

Western blot - Anti-MSH2 antibody [SP46] (AB231437)

False colour image of Western blot : Anti-MSH2 antibody [SP46] staining at 0.061 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab231437 was shown to bind specifically to MSH2. A band was observed at 104 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in MSH2 knockout cell line. To generate this image, wild-type and MSH2 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-MSH2 antibody [SP46] (ab231437) at 0.061 µg/mL

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

Western blot - Human MSH2 knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-msh2-knockout-hct116-cell-line-ab286273'>ab286273</a>)

Lane 2:

MSH2 knockout HCT 116 cell lysate at 20 µg

Lane 3:

HAP1 cell lysate at 20 µg

Lane 4:

A-375 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 105 kDa

Observed band size: 104 kDa

false

Western blot - Anti-MSH2 antibody [SP46] (AB231437)
  • WB

Lab

Western blot - Anti-MSH2 antibody [SP46] (AB231437)

Blocking/Diluting buffer and concentration : 5% NFDM /TBST

All lanes:

Western blot - Anti-MSH2 antibody [SP46] (ab231437) at 0.061 µg/mL

All lanes:

A549 (Human lung carcinoma epithelial cell) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 105 kDa

Observed band size: 104 kDa

false

Western blot - Anti-MSH2 antibody [SP46] (AB231437)
  • WB

Lab

Western blot - Anti-MSH2 antibody [SP46] (AB231437)

Western blot : Anti-MSH2 antibody [SP46] (ab231437) staining at 0.061 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab231437 was shown to bind specifically to MSH2. A band was observed at 104 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in MSH2 knockout cell line. To generate this image, wild-type and MSH2 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-MSH2 antibody [SP46] (ab231437) at 0.061 µg/mL

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

MSH2 knockout HCT 116 cell lysate at 20 µg

Lane 3:

A-375 cell lysate at 20 µg

Observed band size: 104 kDa

false

  • Carrier free

    Anti-MSH2 antibody [SP46] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP46

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB52266

Anti-MSH2 antibody [3A2B8C]

Flow Cytometry - Anti-MSH2 antibody [3A2B8C] (AB52266)

  • 5
  • 3
Primary Antibodies

AB70270

Anti-MSH2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody (AB70270)

  • 5
  • 4
Primary Antibodies

AB92473

Anti-MSH2 antibody [EPR3943]

RabMAb|Recombinant|KO Validated

Western blot - Anti-MSH2 antibody [EPR3943] (AB92473)

  • 4
  • 4
Primary Antibodies

AB245383

Anti-MSH2 antibody

Immunoprecipitation - Anti-MSH2 antibody (AB245383)

  • 0
  • 0
Primary Antibodies

AB227941

Anti-MSH2 antibody [EPR21017-123]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-MSH2 antibody [EPR21017-123] (AB227941)

  • 0
  • 0
Primary Antibodies

AB228334

Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (AB228334)

  • 0
  • 0

Complementary Products

Assay Kits

AB233629

Human MSH2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human MSH2 ELISA Kit (AB233629)

  • 0
  • 0
Primary Antibodies

AB92471

Anti-MSH6 antibody [EPR3945]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [EPR3945] (AB92471)

  • 5
  • 5
Primary Antibodies

AB92312

Anti-MLH1 antibody [EPR3894]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-MLH1 antibody [EPR3894] (AB92312)

  • 5
  • 4
Primary Antibodies

AB110638

Anti-PMS2 antibody [EPR3947]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMS2 antibody [EPR3947] (AB110638)

  • 4
  • 3
Primary Antibodies

AB226079

Anti-MSH3 antibody [EPR4334(2)] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-MSH3 antibody [EPR4334(2)] - BSA and Azide free (AB226079)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p>Primary antibody incubation for 30 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.061 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column

Product details

To see more of the key markers and tools you need to study the hallmarks of cancer, including genome instability and mutation, please visit the following page.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MSH2 also known as MutS Homolog 2 is a human protein with a molecular weight of approximately 100 kDa. It is an important component of the DNA mismatch repair system and plays an essential role in maintaining genomic stability by recognizing and repairing mismatched nucleotides during DNA replication. Expression of the MSH2 protein occurs broadly in dividing cells across various tissues with notable presence in tissues with high proliferation rates such as the colon and the endometrium. Additionally detection and quantification of MSH2 are often performed using methodologies such as MSH2 ELISA which aids in the assessment of its expression levels in different biological samples.
Biological function summary

Components are identified in mismatch repair where MSH2 forms a heterodimer with MSH6 known as the MutSα complex or with MSH3 known as the MutSβ complex. This heterodimerization is critical for the initial steps in the recognition and binding of mismatch errors on the DNA strand. MSH2 complex formation enables it to scan the DNA for errors facilitating the recruitment of additional repair proteins. The activity of MSH2 in these complexes is important in preserving the fidelity of genetic information and prevents mutations that could lead to genomic instability.

Pathways

MSH2 operates within the DNA damage response and repair pathways. The protein is a core component of the mismatch repair pathway which corrects DNA replication errors that elude proofreading activity of DNA polymerases. It interacts with other proteins such as MLH1 and PMS2 forming a synergistic function that amplifies the capacity to recognize and initiate repair of mismatches. The pathway involving MSH2 not only repairs mismatched bases but also plays a role in cell cycle control checkpoints and apoptosis evidencing its pivotal role in maintaining cell cycle integrity.

Studies show that MSH2 is strongly associated with Lynch syndrome an autosomal dominant inherited condition that increases the risk of colorectal cancer. Mutations in the MSH2 gene impair its mismatch repair function and lead to microsatellite instability a hallmark of cancer cells in this disorder. Furthermore alterations in the MSH2 protein also relate to glioblastomas with correlations observed between MSH2 expression levels and tumor progression. These conditions exemplify the important role of MSH2 and its interaction with other DNA repair proteins in preventing cancerous developments.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers : MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand (PubMed : 26300262). ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch : mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
See full target information MSH2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 17:105264 PubMed40697228

2025

Nav1.6 drives colorectal cancer proliferation and invasion through MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ming Zhao,Wan-Ying Hong,Jian-Guang Xu,Shui-Quan Lin,Ming-Sheng Liu,Li-Hui Wang,Xu-Li Jiang,Ming Sang,Yang-Bo Lv
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com