Anti-MSH2 antibody [SP46] - BSA and Azide free

• WB

Lab

Western blot - Anti-MSH2 antibody [SP46] - BSA and Azide free (AB236239)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231437). False colour image of Western blot : Anti-MSH2 antibody [SP46] staining at 0.061 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab231437 was shown to bind specifically to MSH2. A band was observed at 104 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in MSH2 knockout cell line. To generate this image, wild-type and MSH2 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-MSH2 antibody [SP46] (<a href='/en-us/products/primary-antibodies/msh2-antibody-sp46-ab231437'>ab231437</a>) at 0.061 µg/mL

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

Western blot - Human MSH2 knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-msh2-knockout-hct116-cell-line-ab286273'>ab286273</a>)

Lane 2:

MSH2 knockout HCT 116 cell lysate at 20 µg

Lane 3:

HAP1 cell lysate at 20 µg

Lane 4:

A-375 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 105 kDa

Observed band size: 104 kDa

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• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [SP46] - BSA and Azide free (AB236239)

Formalin-fixed, paraffin-embedded human prostate tissue stained for MSH2 using ab231437 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231437).