Rabbit Polyclonal MSH3 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MSH3.
Images
Publications
Filter by
- Nature communications 13:7602022Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Ferreira da Silva,G P Oliveira,E A Arasa-Verge,C Kagiou,A Moretton,G Timelthaler,J Jiricny,J I LoizouPubMed 35140211
- Nucleic acids research 45:10068-100782017MutSβ abundance and Msh3 ATP hydrolysis activity are important drivers of CTG•CAG repeat expansions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNorma Keogh,Kara Y Chan,Guo-Min Li,Robert S LahuePubMed 28973443
- Nucleic acids research 37:4420-92009Mismatch repair and nucleotide excision repair proteins cooperate in the recognition of DNA interstrand crosslinks.Applications:WBReactive species:HumanJunhua Zhao,Aklank Jain,Ravi R Iyer,Paul L Modrich,Karen M VasquezPubMed 19468048
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MSH3. The exact immunogen used to generate this antibody is proprietary information. Database link P20585
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
Alternative names
DUC1, DUG, MSH3, DNA mismatch repair protein Msh3, hMSH3, Divergent upstream protein, Mismatch repair protein 1, DUP, MRP1
Recommended products
Rabbit Polyclonal MSH3 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MSH3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MSH3. The exact immunogen used to generate this antibody is proprietary information. Database link P20585
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The mutS homolog 3 (MSH3) protein plays an important role in DNA mismatch repair. It is part of the family of MutS proteins and is sometimes referred to by its gene name MSH3. MSH3 forms a heterodimer with MSH2 to create MutSβ. This complex targets insertion and deletion loops. The mass of MSH3 is approximately 127 kDa. Expression of MSH3 occurs in various tissues with high levels in the colon small intestine and testis.
- Biological function summary
MSH3 contributes to genome stability by correcting DNA replication errors. As part of the MutSβ complex it identifies and initiates repair of insertion and deletion loops which helps maintain genomic integrity. In eukaryotes MSH3 along with its partner MSH2 cooperates with other proteins such as MLH1 and PMS2 to ensure proper mismatch repair. These interactions help to prevent mutations from becoming permanent.
- Pathways
MSH3 participates in the DNA mismatch repair pathway important for maintaining genomic fidelity. It works closely with MSH2 to form MutSβ enhancing the repair of small loops. Another important association includes the MLH1-PMS2 complex within the mismatch repair pathways. This collaboration ensures errors in DNA during replication do not lead to genomic instability.
- Associated diseases and disorders
Mutations or malfunctioning of MSH3 have been associated with colorectal cancer and Lynch syndrome. Alterations in MSH3 can impair DNA repair processes contributing to cancer development. The MSH2 protein which pairs with MSH3 also plays a critical role in these disorders. Understanding MSH3 function helps illuminate its involvement in genetic stability and its impact on health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-MSH3 antibody (ab69619)
All lanes: Western blot - Anti-MSH3 antibody (ab69619) at 1/500 dilution
Lane 1: Extracts from HUVEC cells at 5 µg
Lane 2: Extracts from HUVEC cells at 5 µg with immunising peptide
Predicted band size: 127 kDa
Observed band size: 100 kDa, 127 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com