AB69619

Anti-MSH3 antibody

Name: Anti-MSH3 antibody (ab69619) | Abcam
Brand: Abcam Limited
SKU: AB69619

Be the first to review this product! Submit a review

(4 Publications)

Rabbit Polyclonal MSH3 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MSH3.

View Alternative Names

DUC1, DUG, MSH3, DNA mismatch repair protein Msh3, hMSH3, Divergent upstream protein, Mismatch repair protein 1, DUP, MRP1

1 Images

Unknown

Western blot - Anti-MSH3 antibody (AB69619)

All lanes:

Western blot - Anti-MSH3 antibody (ab69619) at 1/500 dilution

Lane 1:

Extracts from HUVEC cells at 5 µg

Lane 2:

Extracts from HUVEC cells at 5 µg with immunising peptide

Predicted band size: 127 kDa

Observed band size: 100 kDa,127 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human MSH3. The exact immunogen used to generate this antibody is proprietary information.

P20585

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The mutS homolog 3 (MSH3) protein plays an important role in DNA mismatch repair. It is part of the family of MutS proteins and is sometimes referred to by its gene name MSH3. MSH3 forms a heterodimer with MSH2 to create MutSβ. This complex targets insertion and deletion loops. The mass of MSH3 is approximately 127 kDa. Expression of MSH3 occurs in various tissues with high levels in the colon small intestine and testis.

Biological function summary

MSH3 contributes to genome stability by correcting DNA replication errors. As part of the MutSβ complex it identifies and initiates repair of insertion and deletion loops which helps maintain genomic integrity. In eukaryotes MSH3 along with its partner MSH2 cooperates with other proteins such as MLH1 and PMS2 to ensure proper mismatch repair. These interactions help to prevent mutations from becoming permanent.

Pathways

MSH3 participates in the DNA mismatch repair pathway important for maintaining genomic fidelity. It works closely with MSH2 to form MutSβ enhancing the repair of small loops. Another important association includes the MLH1-PMS2 complex within the mismatch repair pathways. This collaboration ensures errors in DNA during replication do not lead to genomic instability.

Mutations or malfunctioning of MSH3 have been associated with colorectal cancer and Lynch syndrome. Alterations in MSH3 can impair DNA repair processes contributing to cancer development. The MSH2 protein which pairs with MSH3 also plays a critical role in these disorders. Understanding MSH3 function helps illuminate its involvement in genetic stability and its impact on health.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.

See full target information MSH3

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1205250 PubMed37426643

2023

Identification of disulfidptosis related subtypes, characterization of tumor microenvironment infiltration, and development of DRG prognostic prediction model in RCC, in which is a key gene during disulfidptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Xu,Ye Zhang,Zhiwei Yan,Yuchan Wang,Yanze Li,Qiangmin Qiu,Yang Du,Zhiyuan Chen,Xiuheng Liu

Nature communications 13:760 PubMed35140211

2022

Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair.

Applications

Unspecified application

Species

Unspecified reactive species

J Ferreira da Silva,G P Oliveira,E A Arasa-Verge,C Kagiou,A Moretton,G Timelthaler,J Jiricny,J I Loizou

Nucleic acids research 45:10068-10078 PubMed28973443

2017

MutSβ abundance and Msh3 ATP hydrolysis activity are important drivers of CTG•CAG repeat expansions.

Applications

Unspecified application

Species

Unspecified reactive species

Norma Keogh,Kara Y Chan,Guo-Min Li,Robert S Lahue

Nucleic acids research 37:4420-9 PubMed19468048

2009

Mismatch repair and nucleotide excision repair proteins cooperate in the recognition of DNA interstrand crosslinks.

Applications

Species

Human

Junhua Zhao,Aklank Jain,Ravi R Iyer,Paul L Modrich,Karen M Vasquez

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MSH3 antibody

Western blot - Anti-MSH3 antibody (AB69619)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise