AB327152

Anti-MSH3 Antibody [CL14064]

Name: Anti-MSH3 Antibody [CL14064] (ab327152) | Abcam
Brand: Abcam Limited
SKU: AB327152
Price: 580 USD
Availability: InStock

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Mouse Monoclonal MSH3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MSH3.

View Alternative Names

DUC1, DUG, MSH3, DNA mismatch repair protein Msh3, hMSH3, Divergent upstream protein, Mismatch repair protein 1, DUP, MRP1

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH3 Antibody [CL14064] (AB327152)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH3 Antibody [CL14064] (AB327152)

Immunohistochemical staining of human kidney using ab327152 at 1 : 200–1 : 500 dilution, showing moderate nuclear positivity in cells in tubules and cells in glomeruli.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH3 Antibody [CL14064] (AB327152)

Immunohistochemical staining of human breast cancer using ab327152 at 1 : 200–1 : 500 dilution, showing moderate to strong nuclear positivity in tumor cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH3 Antibody [CL14064] (AB327152)

Immunohistochemical staining of human cervical cancer using ab327152 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in tumor cells.

Immunocytochemistry/ Immunofluorescence - Anti-MSH3 Antibody [CL14064] (AB327152)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MSH3 Antibody [CL14064] (AB327152)

Immunofluorescence staining in RH-30 cell line using ab327152 at 2–10 µg/ml, showing specific staining of nucleoplasm and nucleus and nucleoli in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH3 Antibody [CL14064] (AB327152)

Immunohistochemical staining of human uterine cervix using ab327152 at 1 : 200–1 : 500 dilution, showing moderate nuclear positivity in squamous epithelial cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH3 Antibody [CL14064] (AB327152)

Immunohistochemical staining of human colorectal cancer using ab327152 at 1 : 200–1 : 500 dilution, showing moderate to strong nuclear positivity in tumor cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH3 Antibody [CL14064] (AB327152)

Immunohistochemical staining of human cerebellum using ab327152 at 1 : 200–1 : 500 dilution, showing moderate nuclear positivity in Purkinje cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH3 Antibody [CL14064] (AB327152)

Immunohistochemical staining of human endometrium using ab327152 at 1 : 200–1 : 500 dilution, showing moderate nuclear positivity in glandular and in stromal cells.

Supplier Data

Western blot - Anti-MSH3 Antibody [CL14064] (AB327152)

Western blot analysis in human cell line U-251 MG using ab327152 at 1 µg/ml.

All lanes:

Western blot - Anti-MSH3 Antibody [CL14064] (ab327152)

All lanes:

Human cell line U-251 MG

Predicted band size: 127.41 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH3 Antibody [CL14064] (AB327152)

Immunohistochemical staining of human testis using ab327152 at 1 : 200–1 : 500 dilution, showing no positivity in cells in seminiferous ducts.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL14064

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MSH3.

P20585

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.

See full target information MSH3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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