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Rabbit Recombinant Monoclonal MSH3 antibody. Suitable for WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-MSH3 antibody [EPR29050-69] (AB324225), expandable thumbnail
  • Western blot - Anti-MSH3 antibody [EPR29050-69] (AB324225), expandable thumbnail
  • Western blot - Anti-MSH3 antibody [EPR29050-69] (AB324225), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Not recommended
Mouse
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Target data

Function

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis (By similarity).

Alternative names

Rep-3, DNA mismatch repair protein Msh3, Protein repair-1, Protein repair-3, REP-1, REP-3

Rabbit Recombinant Monoclonal MSH3 antibody. Suitable for WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29050-69
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-MSH3 antibody [EPR29050-69] (ab324225), expandable thumbnail

    Western blot - Anti-MSH3 antibody [EPR29050-69] (ab324225)

    MSH3 Western blot staining using rabbit Anti-MSH3 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Performed under reducing conditions.

    In Western blot, ab324225 was shown to bind specifically to MSH3. Target of interest was observed at 120 kDa in wild-type A549 cell lysates (lane 1) with no signal observed at this size in MSH3 knockout cell line (lane 2) (lane 2, knockout cell line Human MSH3 knockout A549 cell line ab288875).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-MSH3 antibody [EPR29050-69] (ab324225) at 1/1000 dilution

    Lane 1: Wild-type A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: MSH3 knockout A549 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 120 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-MSH3 antibody [EPR29050-69] (ab324225), expandable thumbnail

    Western blot - Anti-MSH3 antibody [EPR29050-69] (ab324225)

    MSH3 Western blot staining using rabbit Anti-MSH3 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the band at approximately 36 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-MSH3 antibody [EPR29050-69] (ab324225) at 1/1000 dilution

    Lane 1: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 2: NIH/3T3 (mouse embryonic fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 3: NIH/3T3 transfected with siRNA specifically targeting MSH3 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 120 kDa, 36 kDa

    Exposure time: 26s

  • Western blot - Anti-MSH3 antibody [EPR29050-69] (ab324225), expandable thumbnail

    Western blot - Anti-MSH3 antibody [EPR29050-69] (ab324225)

    MSH3 Western blot staining using rabbit Anti-MSH3 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    To minimize protein degradation, cells (lanes 2-7) were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    Lanes 1 is incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 2-7 are incubated with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000.

    The identity of the band at approximately 36 kDa in lanes 2-7 is unknown.

    The identity of the band at approximately 15 kDa in lanes 1 is unknown.

    The identity of the band at approximately 250 kDa in lanes 2-3 is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1, Lanes 4-7 : 180 seconds; Lanes 2-3: 48 seconds

    All lanes: Western blot - Anti-MSH3 antibody [EPR29050-69] (ab324225) at 1/1000 dilution

    Lane 1: Human pancreas cancer tissue lysate at 20 µg

    Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 5: Mouse brain tissue lysate at 20 µg

    Lane 6: Mouse testis tissue lysate at 20 µg

    Lane 7: Mouse spleen tissue lysate at 20 µg

    Secondary

    Lane 1: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Lanes 2 - 7: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 120 kDa, 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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