Anti-MSH3 antibody [RM405] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH3 antibody [RM405] - BSA and Azide free (AB322511)

This data was developed using ab275928, the same antibody clone in a different buffer formulation.

Formalin-fixed, paraffin-embedded human colon cancer tissue stained for MSH3 using ab275928 at 1/200 dilution in immunohistochemical analysis.

• WB

Supplier Data

Western blot - Anti-MSH3 antibody [RM405] - BSA and Azide free (AB322511)

This data was developed using ab275928, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MSH3 antibody [RM405] (<a href='/en-us/products/primary-antibodies/msh3-antibody-rm405-ab275928'>ab275928</a>) at 1/800 dilution

All lanes:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

Predicted band size: 127 kDa

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• WB

Lab

Western blot - Anti-MSH3 antibody [RM405] - BSA and Azide free (AB322511)

This data was developed using ab275928, the same antibody clone in a different buffer formulation.

False colour image of Western blot : Anti-MSH3 antibody [RM405] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab275928 was shown to bind specifically to MSH3. A band was observed at 140 kDa in wild-type A549 cell lysates with no signal observed at this size in MSH3 knockout cell line ab288875. To generate this image, wild-type and MSH3 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-MSH3 antibody [RM405] (<a href='/en-us/products/primary-antibodies/msh3-antibody-rm405-ab275928'>ab275928</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

MSH3 knockout A549 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

NIH/3T3 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 127 kDa

Observed band size: 140 kDa

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