AB313331

Anti-MSH4 antibody [EPR27244-57]

20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal MSH4 antibody. Suitable for WB, IP and reacts with Mouse samples.

View Alternative Names

MutS protein homolog 4, mMsh4, Msh4

2 Images

Supplier Data

Immunoprecipitation - Anti-MSH4 antibody [EPR27244-57] (AB313331)

MSH4 was immunoprecipitated from 0.35 mg Mouse testis tissue lysate with ab313331 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313331 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse testis tissue lysate Lane 2 : Mouse testis tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab313331 in mouse testis tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 58 seconds

All lanes:

Immunoprecipitation - Anti-MSH4 antibody [EPR27244-57] (ab313331) at 1/30 dilution

All lanes:

Mouse testis tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

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Exposure time: 58s

Supplier Data

Western blot - Anti-MSH4 antibody [EPR27244-57] (AB313331)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : spleen (PMID : 9299235, 10809667, 18947432 ), kidney (PMID : 10809667, 18947432). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-MSH4 antibody [EPR27244-57] (ab313331) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 100 kDa

false

Exposure time: 180s

Carrier free

Anti-MSH4 antibody [EPR27244-57] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27244-57

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "(0.512ug/ml)", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "(0.512ug/ml)", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "(0.512ug/ml)", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MSH4 known as MutS Homolog 4 is a protein involved in meiotic recombination and DNA repair. MSH4 is a member of the DNA mismatch repair (MMR) system which is known for its role in maintaining genome stability. The protein has an estimated mass of around 103 kDa. MSH4 is expressed in the testis and ovary where it localizes to the synaptonemal complex during the early stages of meiosis. It assists in the proper alignment and pairing of homologous chromosomes essential for accurate genetic recombination.

Biological function summary

MSH4 contributes to genome diversity and stability by facilitating the formation and resolution of crossover events during meiosis I. MSH4 forms a heterodimer with MSH5 another MutS homolog which is necessary for recognizing and binding to DNA double-strand breaks. This complex acts as a scaffold for the assembly of other repair proteins required for recombination. The interaction of MSH4-MSH5 complex with important proteins like MLH1 and MLH3 fosters the maturation of crossovers further ensuring genetic diversity and error-free distribution of genetic material.

Pathways

MSH4 plays an important role in meiotic homologous recombination and mismatch repair pathways. Within the homologous recombination pathway MSH4 operates with MSH5 to promote synapsis and crossover formation between homologous chromosomes. This involvement is critical for fertility and genome stability. The protein also interacts with the MLH1 and MLH3 in the repair pathway emphasizing its role in the maintenance and integrity of genetic material during cell division.

Defects in the MSH4 function can lead to anomalies in meiotic recombination increasing the risk for infertility and certain cancers. It has been associated with conditions such as azoospermia where dysfunctional MSH4 may result in improper chromosomal segregation. The interaction of MSH4-MSH5 with other proteins like MLH1 also relates to Lynch syndrome characterized by increased cancer propensity due to mismatch repair deficiencies. Therefore understanding MSH4 and its connections with other proteins is essential for researching therapeutic interventions in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in meiotic recombination. Required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis.

See full target information Msh4

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MSH4 antibody [EPR27244-57]

Immunoprecipitation - Anti-MSH4 antibody [EPR27244-57] (AB313331)

Western blot - Anti-MSH4 antibody [EPR27244-57] (AB313331)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise