Anti-MSH6 antibody [MSH6/3086] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MSH6 antibody [MSH6/3086] - BSA and Azide free (AB259431)

PFA-fixed MCF7 (human breast adenocarcinoma cell line) cells labeling MSH6 using ab259428 at 2 μg/ml (green) in ICC/IF.

The nuclear counterstain is Reddot (red).

The secondary antibody is a Goat anti-mouse IgG CF488 conjugate.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab259428).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [MSH6/3086] - BSA and Azide free (AB259431)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling MSH6 using ab259428 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab259428).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH6 antibody [MSH6/3086] - BSA and Azide free (AB259431)

Formalin-fixed, paraffin-embedded human small intestine tissue labeling MSH6 using ab259428 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab259428).

• WB

Supplier Data

Western blot - Anti-MSH6 antibody [MSH6/3086] - BSA and Azide free (AB259431)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab259428).

All lanes:

Western blot - Anti-MSH6 antibody [MSH6/3086] (<a href='/en-us/products/primary-antibodies/msh6-antibody-msh6-3086-ab259428'>ab259428</a>) at 2 µg/mL

All lanes:

HCT 116 (human colorectal carcinoma cell line) cell lysate

Predicted band size: 153 kDa

false

• Protein Array

Supplier Data

Protein Array - Anti-MSH6 antibody [MSH6/3086] - BSA and Azide free (AB259431)

Protein array containing > 19,000 full-length human proteins, probed using ab259428.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab259428).