AB114107

Anti-MSI2 antibody

Name: Anti-MSI2 antibody (ab114107) | Abcam
Brand: Abcam Limited
SKU: AB114107
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal MSI2 antibody. Suitable for IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MSI2 aa 250-300.

View Alternative Names

RNA-binding protein Musashi homolog 2, Musashi-2, MSI2

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Immunoprecipitation - Anti-MSI2 antibody (AB114107)

Detection of Human MSI2 by Western blot of Immunoprecipitate.
MSI2 was immunoprecipitated from HeLa whole cell lysate, using ab114107 at 6 µg/mg lysate (1 mg for IP, 20% of IP loaded). ab114107 was used for the subsequent WB at 1 µg/ml.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-MSI2 antibody (ab114107)

Predicted band size: 35 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human MSI2 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

Q96DH6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Lizard": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab114107 was affinity purified using an epitope specific to MSI2 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Musashi-2 (MSI2) is a member of the Musashi RNA‐binding protein family also labeled as MSI-2. The protein is approximately 38 kDa in size. It expresses primarily in stem cells and various tumor types including hematopoietic cells and neural tissue. MSI2 plays a role in maintaining stem cell self-renewal and differentiation by regulating mRNA translation through binding to specific target RNAs.

Biological function summary

MSI2 contributes to the regulation of gene expression with a significant impact on cell fate decisions. It acts as part of a post-transcriptional regulatory complex that controls the stability and translation of specific mRNA targets. MSI2 influences pathways involved in stem cell maintenance and differentiation linking it to cellular processes like proliferation and apoptosis. Its activity is important for normal development and the maintenance of undifferentiated cell states.

Pathways

MSI2 has important roles in the Notch and Wnt signaling pathways. These pathways are vital for stem cell maintenance and tissue regeneration. MSI2 regulates components of these pathways interacting with proteins such as beta-catenin in Wnt signaling. This regulation enhances its involvement in controlling cell proliferation and differentiation contributing to signaling processes that determine cellular behavior.

MSI2 is linked to acute myeloid leukemia (AML) and certain types of solid tumors. Overexpression of MSI2 often correlates with poor prognosis in AML where it impacts cancer progression by modulating genes associated with cell growth. Additionally its interaction with oncogenic proteins like BCR-ABL suggests its involvement in cancerogenesis through disease-linked pathways. Research indicates MSI2 as a potential target for therapeutic intervention in such malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).

See full target information MSI2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 294:10969-10986 PubMed31152063

2019

Musashi interaction with poly(A)-binding protein is required for activation of target mRNA translation.

Applications

Unspecified application

Species

Unspecified reactive species

Chad E Cragle,Melanie C MacNicol,Stephanie D Byrum,Linda L Hardy,Samuel G Mackintosh,William A Richardson,Nicola K Gray,Gwen V Childs,Alan J Tackett,Angus M MacNicol

The Journal of biological chemistry 293:15439-15454 PubMed30082317

2018

The SUMO protease SENP1 and the chromatin remodeler CHD3 interact and jointly affect chromatin accessibility and gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando Rodríguez-Castañeda,Roza Berhanu Lemma,Ignacio Cuervo,Mads Bengtsen,Lisa Marie Moen,Marit Ledsaak,Ragnhild Eskeland,Odd Stokke Gabrielsen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MSI2 antibody

Immunoprecipitation - Anti-MSI2 antibody (AB114107)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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