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AB156770

Anti-MSI2 antibody [OTI2F10]

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(2 Publications)

Mouse Monoclonal MSI2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Dog, Rat, African green monkey, Transfected cell lysate - Human, Transfected cell line - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MSI2.

View Alternative Names

RNA-binding protein Musashi homolog 2, Musashi-2, MSI2

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody [OTI2F10] (AB156770)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody [OTI2F10] (AB156770)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody [OTI2F10] (AB156770)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody [OTI2F10] (AB156770)

Paraffin-embedded human prostate tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody [OTI2F10] (AB156770)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody [OTI2F10] (AB156770)

Paraffin-embedded human pancreas tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody [OTI2F10] (AB156770)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody [OTI2F10] (AB156770)

Paraffin-embedded human pancreas adenocarcinoma tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.

Immunocytochemistry/ Immunofluorescence - Anti-MSI2 antibody [OTI2F10] (AB156770)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MSI2 antibody [OTI2F10] (AB156770)

Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling MSI2 with ab156770 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody [OTI2F10] (AB156770)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSI2 antibody [OTI2F10] (AB156770)

Paraffin-embedded human ovary adenocarcinoma tissue stained for MSI2 with ab156770 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 minutes.

Western blot - Anti-MSI2 antibody [OTI2F10] (AB156770)
  • WB

Supplier Data

Western blot - Anti-MSI2 antibody [OTI2F10] (AB156770)

All lanes:

Western blot - Anti-MSI2 antibody [OTI2F10] (ab156770) at 1/200 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 35 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 35 µg

Lane 3:

SVT2 (Mouse cell line) cell lysate at 35 µg

Lane 4:

A549 (Human lung carcinoma cell line) cell lysate at 35 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate at 35 µg

Lane 6:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 35 µg

Lane 7:

MDCK (Canine kidney cell line) cell lysate at 35 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate at 35 µg

Lane 9:

MCF7 (Human breast adenocarcinoma cell line) cell lysate at 35 µg

Predicted band size: 35 kDa

false

Western blot - Anti-MSI2 antibody [OTI2F10] (AB156770)
  • WB

Supplier Data

Western blot - Anti-MSI2 antibody [OTI2F10] (AB156770)

All lanes:

Western blot - Anti-MSI2 antibody [OTI2F10] (ab156770) at 1/4000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY MSI2 cDNA at 5 µg

Predicted band size: 35 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-MSI2 antibody [OTI2F10] (AB156770)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MSI2 antibody [OTI2F10] (AB156770)

Immunofluorescent analysis of COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY MSI2, labeling MSI2 with ab156770 at 1/100 dilution.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2F10

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Dog, Human, African green monkey

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MSI2.

Q96DH6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

The clone number has been updated from 2F10 to OTI2F10, both clone numbers name the same clone.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant by affinity chromatography
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Musashi-2 (MSI2) is a member of the Musashi RNA‐binding protein family also labeled as MSI-2. The protein is approximately 38 kDa in size. It expresses primarily in stem cells and various tumor types including hematopoietic cells and neural tissue. MSI2 plays a role in maintaining stem cell self-renewal and differentiation by regulating mRNA translation through binding to specific target RNAs.
Biological function summary

MSI2 contributes to the regulation of gene expression with a significant impact on cell fate decisions. It acts as part of a post-transcriptional regulatory complex that controls the stability and translation of specific mRNA targets. MSI2 influences pathways involved in stem cell maintenance and differentiation linking it to cellular processes like proliferation and apoptosis. Its activity is important for normal development and the maintenance of undifferentiated cell states.

Pathways

MSI2 has important roles in the Notch and Wnt signaling pathways. These pathways are vital for stem cell maintenance and tissue regeneration. MSI2 regulates components of these pathways interacting with proteins such as beta-catenin in Wnt signaling. This regulation enhances its involvement in controlling cell proliferation and differentiation contributing to signaling processes that determine cellular behavior.

MSI2 is linked to acute myeloid leukemia (AML) and certain types of solid tumors. Overexpression of MSI2 often correlates with poor prognosis in AML where it impacts cancer progression by modulating genes associated with cell growth. Additionally its interaction with oncogenic proteins like BCR-ABL suggests its involvement in cancerogenesis through disease-linked pathways. Research indicates MSI2 as a potential target for therapeutic intervention in such malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
See full target information MSI2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 21: PubMed31878037

2019

Immunohistochemical Analysis Revealed a Correlation between Musashi-2 and Cyclin-D1 Expression in Patients with Oral Squamous Cells Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppe Troiano,Vito Carlo Alberto Caponio,Gerardo Botti,Gabriella Aquino,Nunzia Simona Losito,Maria Carmela Pedicillo,Khrystyna Zhurakivska,Claudia Arena,Domenico Ciavarella,Filiberto Mastrangelo,Lucio Lo Russo,Lorenzo Lo Muzio,Giuseppe Pannone

Oncogene 36:2802-2812 PubMed27941885

2016

DBC2/RhoBTB2 functions as a tumor suppressor protein via Musashi-2 ubiquitination in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Y M Choi,K B Kim,J H Lee,Y K Chun,I S An,S An,S Bae
View all publications
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