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Rabbit Monoclonal mSin3A antibody. Suitable for IP, Flow Cyt (Intra), WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

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Images

Western blot - Anti-mSin3a antibody [EPR27103-53] (AB307197), expandable thumbnail
  • Western blot - Anti-mSin3a antibody [EPR27103-53] (AB307197), expandable thumbnail
  • Western blot - Anti-mSin3a antibody [EPR27103-53] (AB307197), expandable thumbnail
  • Immunoprecipitation - Anti-mSin3a antibody [EPR27103-53] (AB307197), expandable thumbnail
  • Western blot - Anti-mSin3a antibody [EPR27103-53] (AB307197), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPIPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Not recommended
Tested
Tested
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Tested
Rat
Not recommended
Expected
Expected
Not recommended
Not recommended
Tested

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression (By similarity). Required for cortical neuron differentiation and callosal axon elongation (By similarity).

Alternative names

Paired amphipathic helix protein Sin3a, Histone deacetylase complex subunit Sin3a, Transcriptional corepressor Sin3a, SIN3A

Recommended products

Rabbit Monoclonal mSin3A antibody. Suitable for IP, Flow Cyt (Intra), WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27103-53
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MSin3A also known as Sin3A is a transcriptional corepressor that plays an important role in regulating gene expression. It weighs approximately 155 kDa. You can find mSin3A expressed in various tissues throughout the body. This protein does not bind DNA directly but associates with DNA-binding proteins to exert its effects. mSin3A functions mechanically by interacting with histone deacetylases (HDACs) leading to the remodeling of chromatin and repression of gene transcription.

Biological function summary

MSin3A acts to regulate gene silencing and is a component of the mSin3A/HDAC complex. This complex is involved in the control of cell cycle progression differentiation and apoptosis. In cellular processes mSin3A serves as a scaffold binding various proteins to create a platform for gene regulatory functions. Its role in recruiting HDACs to specific genomic locations aids in transcriptional repression affecting many biological processes.

Pathways

MSin3A plays significant roles in the Notch and p53 signaling pathways. These pathways are essential in controlling cell fate decisions and responses to stress. mSin3A partners with HDAC1 and HDAC2 within these pathways facilitating the deacetylation and repression of target genes. In the Notch signaling pathway mSin3A interacts with repressors like CSL while in the p53 pathway it influences p53-dependent transcriptional outcomes by modifying the chromatin state.

Associated diseases and disorders

MSin3A shows connection to cancer and neurodegenerative diseases. In cancer mSin3A affects tumor suppression through its interaction with p53 playing a role in cell cycle arrest and apoptosis. In neurodegenerative diseases mSin3A deregulation may disrupt chromatin architecture impairing neuronal function. By targeting mSin3A and associated proteins like HDAC1 researchers explore potential therapeutic avenues for these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-mSin3a antibody [EPR27103-53] (ab307197), expandable thumbnail

    Western blot - Anti-mSin3a antibody [EPR27103-53] (ab307197)

    MSin3a Western blot staining using rabbit Anti-mSin3a antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-mSin3a antibody [EPR27103-53] (ab307197) at 1/1000 dilution

    Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 2: Human testis tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 170 kDa

    Exposure time: 40s

  • Western blot - Anti-mSin3a antibody [EPR27103-53] (ab307197), expandable thumbnail

    Western blot - Anti-mSin3a antibody [EPR27103-53] (ab307197)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the bands below 75 kDa are unknown.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-mSin3a antibody [EPR27103-53] (ab307197) at 1/1000 dilution

    Lane 1: C6 whole cell lysate at 20 µg

    Lane 2: RAW264.7 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 170 kDa

    Exposure time: 180s

  • Western blot - Anti-mSin3a antibody [EPR27103-53] (ab307197), expandable thumbnail

    Western blot - Anti-mSin3a antibody [EPR27103-53] (ab307197)

    Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.

    Lysates at 20 µg per lane.

    The samples were run on a Bis-Tris gel.




    Performed under reducing conditions.

    False colour image of Western blot: Anti-mSin3A antibody (ab307197) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody 6C5 (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.

    In Western blot, ab307197 was shown to bind specifically to mSin3A. A band was observed at 170 kDa in wild-type HAP1 cell lysates with no signal observed at this size in mSin3A knockout cell line. To generate this image, wild-type and mSin3A knockout HAP1 cell lysates were analyzed. First, samples were run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in in Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-mSin3a antibody [EPR27103-53] (ab307197) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: mSin3A knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HeLa whole cell lysate at 20 µg

    Secondary

    Lanes 1 - 3: Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution

    Lanes 1 - 3: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 170 kDa

  • Immunoprecipitation - Anti-mSin3a antibody [EPR27103-53] (ab307197), expandable thumbnail

    Immunoprecipitation - Anti-mSin3a antibody [EPR27103-53] (ab307197)

    mSin3A was immunoprecipitated from 0.35 mg HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate 10 µg with ab307197 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307197 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HAP1 whole cell lysate 10 µg

    Lane 2: ab307197 IP in HAP1 whole cell lysate 10 ug

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307197 in HAP1 cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 6 seconds.

    All lanes: Immunoprecipitation - Anti-mSin3a antibody [EPR27103-53] (ab307197) at 1/1000 dilution

    Lanes 1 - 2: HAP1 whole cell lysate at 10 µg

    Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 170 kDa

    Exposure time: 6s

  • Western blot - Anti-mSin3a antibody [EPR27103-53] (ab307197), expandable thumbnail

    Western blot - Anti-mSin3a antibody [EPR27103-53] (ab307197)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the bands below 75 kDa are unknown.

    The blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 180 seconds.

    All lanes: Western blot - Anti-mSin3a antibody [EPR27103-53] (ab307197) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 20 µg

    Lane 2: Mouse testis tissue lysate at 20 µg

    Lane 3: K-562 whole cell lysate at 20 µg

    Lane 4: 293T whole cell lysate at 20 µg

    Lane 5: F9 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 170 kDa

    Exposure time: 180s

  • Flow Cytometry (Intracellular) - Anti-mSin3a antibody [EPR27103-53] (ab307197), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-mSin3a antibody [EPR27103-53] (ab307197)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) (Right panel) compared to mSin3A knockout HAP1 (Left panel) cells labeling mSin3A with ab307197 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-mSin3a antibody [EPR27103-53] (ab307197), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-mSin3a antibody [EPR27103-53] (ab307197)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labeling mSin3A with ab307197 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

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