Rabbit Monoclonal mSin3A antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), WB and reacts with Human, Rat, Mouse samples.
Images
![Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (AB307198), expandable thumbnail](https://content.abcam.com/products/images/msin3a-antibody-epr27103-53-bsa-and-azide-free-ab307198--western-blot-img445640.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (AB307198)")
![Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (AB307198), expandable thumbnail](https://content.abcam.com/products/images/msin3a-antibody-epr27103-53-bsa-and-azide-free-ab307198--western-blot-img405148.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (AB307198)")
![Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (AB307198), expandable thumbnail](https://content.abcam.com/products/images/msin3a-antibody-epr27103-53-bsa-and-azide-free-ab307198--western-blot-img405150.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (AB307198)")
![Immunoprecipitation - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (AB307198), expandable thumbnail](https://content.abcam.com/products/images/msin3a-antibody-epr27103-53-bsa-and-azide-free-ab307198--immunoprecipitation-img405147.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (AB307198)")
![Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (AB307198), expandable thumbnail](https://content.abcam.com/products/images/msin3a-antibody-epr27103-53-bsa-and-azide-free-ab307198--western-blot-img405149.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (AB307198)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIP | IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Expected | Expected | Not recommended | Not recommended | Tested |
ChIP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression (By similarity). Required for cortical neuron differentiation and callosal axon elongation (By similarity).
Alternative names
Paired amphipathic helix protein Sin3a, Histone deacetylase complex subunit Sin3a, Transcriptional corepressor Sin3a, SIN3A
Recommended products
Rabbit Monoclonal mSin3A antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), WB and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27103-53
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MSin3A also known as Sin3A is a transcriptional corepressor that plays an important role in regulating gene expression. It weighs approximately 155 kDa. You can find mSin3A expressed in various tissues throughout the body. This protein does not bind DNA directly but associates with DNA-binding proteins to exert its effects. mSin3A functions mechanically by interacting with histone deacetylases (HDACs) leading to the remodeling of chromatin and repression of gene transcription.
- Biological function summary
MSin3A acts to regulate gene silencing and is a component of the mSin3A/HDAC complex. This complex is involved in the control of cell cycle progression differentiation and apoptosis. In cellular processes mSin3A serves as a scaffold binding various proteins to create a platform for gene regulatory functions. Its role in recruiting HDACs to specific genomic locations aids in transcriptional repression affecting many biological processes.
- Pathways
MSin3A plays significant roles in the Notch and p53 signaling pathways. These pathways are essential in controlling cell fate decisions and responses to stress. mSin3A partners with HDAC1 and HDAC2 within these pathways facilitating the deacetylation and repression of target genes. In the Notch signaling pathway mSin3A interacts with repressors like CSL while in the p53 pathway it influences p53-dependent transcriptional outcomes by modifying the chromatin state.
- Associated diseases and disorders
MSin3A shows connection to cancer and neurodegenerative diseases. In cancer mSin3A affects tumor suppression through its interaction with p53 playing a role in cell cycle arrest and apoptosis. In neurodegenerative diseases mSin3A deregulation may disrupt chromatin architecture impairing neuronal function. By targeting mSin3A and associated proteins like HDAC1 researchers explore potential therapeutic avenues for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198), expandable thumbnail](https://content.abcam.com/products/images/msin3a-antibody-epr27103-53-bsa-and-azide-free-ab307198--western-blot-img445640.jpg "Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198)")
Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198)
MSin3a Western blot staining using rabbit Anti-mSin3a antibody
This data was developed using Anti-mSin3a antibody [EPR27103-53] ab307197, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-mSin3a antibody [EPR27103-53] (Anti-mSin3a antibody [EPR27103-53] ab307197) at 1/1000 dilution
Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Lane 2: Human testis tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 170 kDa
Exposure time: 40s
![Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198), expandable thumbnail](https://content.abcam.com/products/images/msin3a-antibody-epr27103-53-bsa-and-azide-free-ab307198--western-blot-img405148.jpg "Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198)")
Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198)
This data was developed using Anti-mSin3a antibody [EPR27103-53] ab307197, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the bands below 75 kDa are unknown.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-mSin3a antibody [EPR27103-53] (Anti-mSin3a antibody [EPR27103-53] ab307197) at 1/1000 dilution
Lane 1: C6 whole cell lysate at 20 µg
Lane 2: RAW264.7 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 170 kDa
Exposure time: 180s
![Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198), expandable thumbnail](https://content.abcam.com/products/images/msin3a-antibody-epr27103-53-bsa-and-azide-free-ab307198--western-blot-img405150.jpg "Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198)")
Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198)
This data was developed using Anti-mSin3a antibody [EPR27103-53] ab307197, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.
Lysates at 20 µg per lane.
The samples were run on a Bis-Tris gel.
Performed under reducing conditions.
False colour image of Western blot: Anti-mSin3A antibody (Anti-mSin3a antibody [EPR27103-53] ab307197) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody 6C5 (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.
In Western blot, Anti-mSin3a antibody [EPR27103-53] ab307197 was shown to bind specifically to mSin3A. A band was observed at 170 kDa in wild-type HAP1 cell lysates with no signal observed at this size in mSin3A knockout cell line. To generate this image, wild-type and mSin3A knockout HAP1 cell lysates were analyzed. First, samples were run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in in Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.All lanes: Western blot - Anti-mSin3a antibody [EPR27103-53] (Anti-mSin3a antibody [EPR27103-53] ab307197) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: mSin3A knockout HAP1 whole cell lysate at 20 µg
Lane 3: HeLa whole cell lysate at 20 µg
SecondaryLanes 1 - 3: Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution
Lanes 1 - 3: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 170 kDa
![Immunoprecipitation - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198), expandable thumbnail](https://content.abcam.com/products/images/msin3a-antibody-epr27103-53-bsa-and-azide-free-ab307198--immunoprecipitation-img405147.jpg "Immunoprecipitation - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198)")
Immunoprecipitation - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198)
This data was developed using Anti-mSin3a antibody [EPR27103-53] ab307197, the same antibody clone in a different buffer formulation.
mSin3A was immunoprecipitated from 0.35 mg HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate 10 µg with Anti-mSin3a antibody [EPR27103-53] ab307197 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-mSin3a antibody [EPR27103-53] ab307197 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HAP1 whole cell lysate 10 µg
Lane 2: Anti-mSin3a antibody [EPR27103-53] ab307197 IP in HAP1 whole cell lysate 10 ug
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-mSin3a antibody [EPR27103-53] ab307197 in HAP1 cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 6 seconds.All lanes: Immunoprecipitation - Anti-mSin3a antibody [EPR27103-53] (Anti-mSin3a antibody [EPR27103-53] ab307197) at 1/1000 dilution
Lanes 1 - 2: HAP1 whole cell lysate at 10 µg
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 170 kDa
Exposure time: 6s
![Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198), expandable thumbnail](https://content.abcam.com/products/images/msin3a-antibody-epr27103-53-bsa-and-azide-free-ab307198--western-blot-img405149.jpg "Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198)")
Western blot - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198)
This data was developed using Anti-mSin3a antibody [EPR27103-53] ab307197, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the bands below 75 kDa are unknown.
The blot was developed using a higher sensitivity ECL substrate.
Exposure time: 180 seconds.All lanes: Western blot - Anti-mSin3a antibody [EPR27103-53] (Anti-mSin3a antibody [EPR27103-53] ab307197) at 1/1000 dilution
Lane 1: Human testis tissue lysate at 20 µg
Lane 2: Mouse testis tissue lysate at 20 µg
Lane 3: K-562 whole cell lysate at 20 µg
Lane 4: 293T whole cell lysate at 20 µg
Lane 5: F9 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 170 kDa
Exposure time: 180s
![Flow Cytometry (Intracellular) - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198), expandable thumbnail](https://content.abcam.com/products/images/msin3a-antibody-epr27103-53-bsa-and-azide-free-ab307198--flow-cytometry-intracellular-img405146.jpg "Flow Cytometry (Intracellular) - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198)")
Flow Cytometry (Intracellular) - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198)
This data was developed using Anti-mSin3a antibody [EPR27103-53] ab307197, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) (Right panel) compared to mSin3A knockout HAP1 (Left panel) cells labeling mSin3A with Anti-mSin3a antibody [EPR27103-53] ab307197 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.![Flow Cytometry (Intracellular) - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198), expandable thumbnail](https://content.abcam.com/products/images/msin3a-antibody-epr27103-53-bsa-and-azide-free-ab307198--flow-cytometry-intracellular-img405145.jpg "Flow Cytometry (Intracellular) - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198)")
Flow Cytometry (Intracellular) - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198)
This data was developed using Anti-mSin3a antibody [EPR27103-53] ab307197, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labeling mSin3A with Anti-mSin3a antibody [EPR27103-53] ab307197 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
![Flow Cytometry (Intracellular) - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198), expandable thumbnail](https://content.abcam.com/products/images/msin3a-antibody-epr27103-53-bsa-and-azide-free-ab307198--flow-cytometry-intracellular-img405146.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198)")
![Flow Cytometry (Intracellular) - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198), expandable thumbnail](https://content.abcam.com/products/images/msin3a-antibody-epr27103-53-bsa-and-azide-free-ab307198--flow-cytometry-intracellular-img405145.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-mSin3a antibody [EPR27103-53] - BSA and Azide free (ab307198)")
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