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AB248813

Anti-mSin3A antibody [EPR6779] - BSA and Azide free

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Rabbit Recombinant Monoclonal mSin3A antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Paired amphipathic helix protein Sin3a, Histone deacetylase complex subunit Sin3a, Transcriptional corepressor Sin3a, SIN3A

1 Images
Western blot - Anti-mSin3A antibody [EPR6779] - BSA and Azide free (AB248813)
  • WB

Unknown

Western blot - Anti-mSin3A antibody [EPR6779] - BSA and Azide free (AB248813)

This data was developed using ab137090, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-mSin3A antibody [EPR6779] - BSA and Azide free (ab248813) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 145 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6779

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248813 is the carrier-free version of ab137090.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MSin3A also known as Sin3A is a transcriptional corepressor that plays an important role in regulating gene expression. It weighs approximately 155 kDa. You can find mSin3A expressed in various tissues throughout the body. This protein does not bind DNA directly but associates with DNA-binding proteins to exert its effects. mSin3A functions mechanically by interacting with histone deacetylases (HDACs) leading to the remodeling of chromatin and repression of gene transcription.
Biological function summary

MSin3A acts to regulate gene silencing and is a component of the mSin3A/HDAC complex. This complex is involved in the control of cell cycle progression differentiation and apoptosis. In cellular processes mSin3A serves as a scaffold binding various proteins to create a platform for gene regulatory functions. Its role in recruiting HDACs to specific genomic locations aids in transcriptional repression affecting many biological processes.

Pathways

MSin3A plays significant roles in the Notch and p53 signaling pathways. These pathways are essential in controlling cell fate decisions and responses to stress. mSin3A partners with HDAC1 and HDAC2 within these pathways facilitating the deacetylation and repression of target genes. In the Notch signaling pathway mSin3A interacts with repressors like CSL while in the p53 pathway it influences p53-dependent transcriptional outcomes by modifying the chromatin state.

MSin3A shows connection to cancer and neurodegenerative diseases. In cancer mSin3A affects tumor suppression through its interaction with p53 playing a role in cell cycle arrest and apoptosis. In neurodegenerative diseases mSin3A deregulation may disrupt chromatin architecture impairing neuronal function. By targeting mSin3A and associated proteins like HDAC1 researchers explore potential therapeutic avenues for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression (By similarity). Required for cortical neuron differentiation and callosal axon elongation (By similarity).
See full target information SIN3A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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