AB239899

Anti-MSK1 (phospho S360) antibody [EP1888Y] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal MSK1 phospho S360 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MSK1, RPS6KA5, Ribosomal protein S6 kinase alpha-5, S6K-alpha-5, 90 kDa ribosomal protein S6 kinase 5, Nuclear mitogen- and stress-activated protein kinase 1, RSK-like protein kinase, RSKL

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S360) antibody [EP1888Y] - BSA and Azide free (AB239899)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S360) antibody [EP1888Y] - BSA and Azide free (AB239899)

ICC/IF image of ab81294 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81294, neat) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81294).

Unconjugated

Anti-MSK1 (phospho S360) antibody [EP1888Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1888Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab239899 is the carrier-free version of ab81294.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The mitogen- and stress-activated protein kinase 1 known as MSK1 or RPS6KA5 acts as a serine/threonine kinase. It has a mass of approximately 92 kDa. MSK1 phosphorylates various substrates including proteins involved in gene expression regulation. Researchers find MSK1 expressed mainly in the brain but it appears also in kidneys and lungs. Phosphorylation by MSK1 influences transcription factors like CREB and ATF1 affecting cellular responses to stimuli.

Biological function summary

MSK1 influences cell proliferation and survival. It operates as part of the MAPK signaling pathway driven by extracellular signals. MSK1 activation occurs through ERK1/2 and p38 MAPK therefore linking it to essential cellular processes. As a kinase MSK1 phosphorylates downstream targets contributing to inflammatory responses apoptosis and gene expression modulation. MSK1 often partners with other proteins to form complexes enhancing its regulatory capabilities.

Pathways

MSK1 plays a role in both the MAPK and NF-kB pathways. These pathways contribute to controlling cell growth and immune responses. In the MAPK pathway the involvement of MSK1 influences further regulation of transcription factors. In connection with the NF-kB pathway MSK1 can regulate NF-kB activity promoting gene expression related to inflammation. Related proteins in these pathways include ERK1/2 which activate MSK1 enhancing the kinase's function in these critical cellular circuits.

MSK1 links to various conditions including cancer and neurodegenerative diseases. The dysregulation of MSK1 activity is associated with processes like uncontrolled cell proliferation and abnormal inflammation factors that contribute to tumor development and progression. In neurodegenerative diseases changes in MSK1 activity disrupt neuronal survival pathways affecting cognitive functions. MSK1 connects with proteins like CREB influencing the expression of genes relevant to these diseases' development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes (PubMed : 11909979, PubMed : 12569367, PubMed : 12763138, PubMed : 18511904, PubMed : 9687510, PubMed : 9873047). Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin (PubMed : 11909979, PubMed : 9873047). Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression (PubMed : 12628924, PubMed : 18511904). In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress (PubMed : 12628924). In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential (PubMed : 12763138). Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation (PubMed : 12569367). Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A (PubMed : 15010469). Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN (PubMed : 12773393). May also phosphorylate 'Ser-28' of histone H3 (PubMed : 12773393). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14) (PubMed : 12773393). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines (By similarity). Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors (By similarity). Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury (By similarity). Phosphorylates TRIM7 at 'Ser-107' in response to growth factor signaling via the MEK/ERK pathway, thereby stimulating its ubiquitin ligase activity (PubMed : 25851810).

See full target information RPS6KA5 pS360

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biochemical and biophysical research communications 631:138-145 PubMed36183555

2022

SARS-CoV-2-induced hypomethylation of the ferritin heavy chain (FTH1) gene underlies serum hyperferritinemia in severe COVID-19 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Jibran Sualeh Muhammad,Gehad ElGhazali,Jasmin Shafarin,Mohammad G Mohammad,Ameera Abu-Qiyas,Mawieh Hamad

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MSK1 (phospho S360) antibody [EP1888Y] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S360) antibody [EP1888Y] - BSA and Azide free (AB239899)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise