Rabbit Recombinant Monoclonal MSK1 phospho S376 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Images
![Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529), expandable thumbnail](https://content.abcam.com/products/images/msk1-phospho-s376-antibody-e375-bsa-and-azide-free-ab210529--western-blot-img2204.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529)")
![Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529), expandable thumbnail](https://content.abcam.com/products/images/msk1-phospho-s376-antibody-e375-bsa-and-azide-free-ab210529--immunocytochemistry-immunofluorescence-img10447.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529)")
![Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529), expandable thumbnail](https://content.abcam.com/products/images/msk1-phospho-s376-antibody-e375-bsa-and-azide-free-ab210529--western-blot-img21219.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529)")
![Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529), expandable thumbnail](https://content.abcam.com/products/images/msk1-phospho-s376-antibody-e375-bsa-and-azide-free-ab210529--western-blot-img57663.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529), expandable thumbnail](https://content.abcam.com/products/images/msk1-phospho-s376-antibody-e375-bsa-and-azide-free-ab210529--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127043.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested |
| Rat | Predicted | Predicted | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
Target data
Function
Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes (PubMed:11909979, PubMed:12569367, PubMed:12763138, PubMed:18511904, PubMed:9687510, PubMed:9873047). Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin (PubMed:11909979, PubMed:9873047). Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression (PubMed:12628924, PubMed:18511904). In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress (PubMed:12628924). In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential (PubMed:12763138). Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation (PubMed:12569367). Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A (PubMed:15010469). Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN (PubMed:12773393). May also phosphorylate 'Ser-28' of histone H3 (PubMed:12773393). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14) (PubMed:12773393). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines (By similarity). Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors (By similarity). Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury (By similarity). Phosphorylates TRIM7 at 'Ser-107' in response to growth factor signaling via the MEK/ERK pathway, thereby stimulating its ubiquitin ligase activity (PubMed:25851810).
Alternative names
MSK1, RPS6KA5, Ribosomal protein S6 kinase alpha-5, S6K-alpha-5, 90 kDa ribosomal protein S6 kinase 5, Nuclear mitogen- and stress-activated protein kinase 1, RSK-like protein kinase, RSKL
Recommended products
Rabbit Recombinant Monoclonal MSK1 phospho S376 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- E375
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab210529 is the carrier-free version of Anti-MSK1 (phospho S376) antibody [E375] ab32190.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The mitogen- and stress-activated protein kinase 1 known as MSK1 or RPS6KA5 acts as a serine/threonine kinase. It has a mass of approximately 92 kDa. MSK1 phosphorylates various substrates including proteins involved in gene expression regulation. Researchers find MSK1 expressed mainly in the brain but it appears also in kidneys and lungs. Phosphorylation by MSK1 influences transcription factors like CREB and ATF1 affecting cellular responses to stimuli.
- Biological function summary
MSK1 influences cell proliferation and survival. It operates as part of the MAPK signaling pathway driven by extracellular signals. MSK1 activation occurs through ERK1/2 and p38 MAPK therefore linking it to essential cellular processes. As a kinase MSK1 phosphorylates downstream targets contributing to inflammatory responses apoptosis and gene expression modulation. MSK1 often partners with other proteins to form complexes enhancing its regulatory capabilities.
- Pathways
MSK1 plays a role in both the MAPK and NF-kB pathways. These pathways contribute to controlling cell growth and immune responses. In the MAPK pathway the involvement of MSK1 influences further regulation of transcription factors. In connection with the NF-kB pathway MSK1 can regulate NF-kB activity promoting gene expression related to inflammation. Related proteins in these pathways include ERK1/2 which activate MSK1 enhancing the kinase's function in these critical cellular circuits.
- Associated diseases and disorders
MSK1 links to various conditions including cancer and neurodegenerative diseases. The dysregulation of MSK1 activity is associated with processes like uncontrolled cell proliferation and abnormal inflammation factors that contribute to tumor development and progression. In neurodegenerative diseases changes in MSK1 activity disrupt neuronal survival pathways affecting cognitive functions. MSK1 connects with proteins like CREB influencing the expression of genes relevant to these diseases' development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529), expandable thumbnail](https://content.abcam.com/products/images/msk1-phospho-s376-antibody-e375-bsa-and-azide-free-ab210529--western-blot-img2204.jpg "Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)")
Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
This data was developed using Anti-MSK1 (phospho S376) antibody [E375] ab32190, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Observed bands: 90, 80 kDa.
The 80 kDa band may represent isoform 2 or isoform 3 of MSK1.
All lanes: Western blot - Anti-MSK1 (phospho S376) antibody [E375] (Anti-MSK1 (phospho S376) antibody [E375] ab32190) at 1/2000 dilution
Lane 1: HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates at 10 µg
Lane 2: HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates at 10 µg with MSK1 (phosphor S376) peptide
Lane 3: HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates at 10 µg with MSK1 non-phospho peptide
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 90 kDa
Exposure time: 10s
![Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529), expandable thumbnail](https://content.abcam.com/products/images/msk1-phospho-s376-antibody-e375-bsa-and-azide-free-ab210529--immunocytochemistry-immunofluorescence-img10447.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)")
Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling MSK1 (phospho S376) with Anti-MSK1 (phospho S376) antibody [E375] ab32190 at 1/500 (4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as Nuclear counterstain.
Confocal image showing decreased nuclear staining in HepG2 cells treated with Alkaline Phosphatase (37℃ 1h).
This data was developed using Anti-MSK1 (phospho S376) antibody [E375] ab32190, the same antibody clone in a different buffer formulation.
![Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529), expandable thumbnail](https://content.abcam.com/products/images/msk1-phospho-s376-antibody-e375-bsa-and-azide-free-ab210529--western-blot-img21219.jpg "Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)")
Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
This data was developed using Anti-MSK1 (phospho S376) antibody [E375] ab32190, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Observed band: 90, 80 kDa.
The 80 kDa band may represent isoform 2 or isoform 3 of MSK1.
All lanes: Western blot - Anti-MSK1 (phospho S376) antibody [E375] (Anti-MSK1 (phospho S376) antibody [E375] ab32190) at 1/2000 dilution
Lane 1: Untreated HEK293 (human embryonic kidney) whole cell lysate at 10 µg
Lane 2: HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysate at 10 µg
Lane 3: HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysate. Then the membrane was incubated with Alkaline phosphatase. at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 90 kDa
Exposure time: 5s
![Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529), expandable thumbnail](https://content.abcam.com/products/images/msk1-phospho-s376-antibody-e375-bsa-and-azide-free-ab210529--western-blot-img57663.jpg "Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)")
Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
This data was developed using Anti-MSK1 (phospho S376) antibody [E375] ab32190, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-MSK1 (phospho S376) antibody [E375] (Anti-MSK1 (phospho S376) antibody [E375] ab32190) at 1/5000 dilution
Lane 1: Untreated K562 cell lysate
Lane 2: K562 cell lysate treated with Alkaline
Predicted band size: 90 kDa
Observed band size: 87 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529), expandable thumbnail](https://content.abcam.com/products/images/msk1-phospho-s376-antibody-e375-bsa-and-azide-free-ab210529--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127043.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (ab210529)
This data was developed using Anti-MSK1 (phospho S376) antibody [E375] ab32190, the same antibody clone in a different buffer formulation.Anti-MSK1 (phospho S376) antibody [E375] ab32190, at a dilution of 1/50, staining MSK1 in paraffin embedded human urinary bladder carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com