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AB210529

Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MSK1 phospho S376 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MSK1, RPS6KA5, Ribosomal protein S6 kinase alpha-5, S6K-alpha-5, 90 kDa ribosomal protein S6 kinase 5, Nuclear mitogen- and stress-activated protein kinase 1, RSK-like protein kinase, RSKL

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529)

This data was developed using ab32190, the same antibody clone in a different buffer formulation.

ab32190, at a dilution of 1/50, staining MSK1 in paraffin embedded human urinary bladder carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529)

Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling MSK1 (phospho S376) with ab32190 at 1/500 (4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab150077 Alexa Fluor® 488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as Nuclear counterstain.

Confocal image showing decreased nuclear staining in HepG2 cells treated with Alkaline Phosphatase (37°C 1h).

This data was developed using ab32190, the same antibody clone in a different buffer formulation.

Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529)
  • WB

Lab

Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529)

This data was developed using ab32190, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Observed bands : 90, 80 kDa.

The 80 kDa band may represent isoform 2 or isoform 3 of MSK1.

All lanes:

Western blot - Anti-MSK1 (phospho S376) antibody [E375] (<a href='/en-us/products/primary-antibodies/msk1-phospho-s376-antibody-e375-ab32190'>ab32190</a>) at 1/2000 dilution

Lane 1:

HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates at 10 µg

Lane 2:

HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates at 10 µg with MSK1 (phosphor S376) peptide

Lane 3:

HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates at 10 µg with MSK1 non-phospho peptide

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 90 kDa

false

Exposure time: 10s

Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529)
  • WB

Unknown

Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529)

This data was developed using ab32190, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MSK1 (phospho S376) antibody [E375] (<a href='/en-us/products/primary-antibodies/msk1-phospho-s376-antibody-e375-ab32190'>ab32190</a>) at 1/5000 dilution

Lane 1:

Untreated K562 cell lysate

Lane 2:

K562 cell lysate treated with Alkaline

Predicted band size: 90 kDa

Observed band size: 87 kDa

false

Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529)
  • WB

Lab

Western blot - Anti-MSK1 (phospho S376) antibody [E375] - BSA and Azide free (AB210529)

This data was developed using ab32190, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Observed band : 90, 80 kDa.

The 80 kDa band may represent isoform 2 or isoform 3 of MSK1.

All lanes:

Western blot - Anti-MSK1 (phospho S376) antibody [E375] (<a href='/en-us/products/primary-antibodies/msk1-phospho-s376-antibody-e375-ab32190'>ab32190</a>) at 1/2000 dilution

Lane 1:

Untreated HEK293 (human embryonic kidney) whole cell lysate at 10 µg

Lane 2:

HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysate at 10 µg

Lane 3:

HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysate. Then the membrane was incubated with Alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 90 kDa

false

Exposure time: 5s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

E375

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Primary Antibodies

AB32515

Anti-CREB antibody [E306]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB antibody [E306] (AB32515)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB32360

Anti-NFkB p105 / p50 antibody [E381]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody [E381] (AB32360)

  • 4
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Primary Antibodies

AB97726

Anti-NF-kB p65 (phospho S529) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S529) antibody (AB97726)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab210529 is the carrier-free version of ab32190.

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The mitogen- and stress-activated protein kinase 1 known as MSK1 or RPS6KA5 acts as a serine/threonine kinase. It has a mass of approximately 92 kDa. MSK1 phosphorylates various substrates including proteins involved in gene expression regulation. Researchers find MSK1 expressed mainly in the brain but it appears also in kidneys and lungs. Phosphorylation by MSK1 influences transcription factors like CREB and ATF1 affecting cellular responses to stimuli.
Biological function summary

MSK1 influences cell proliferation and survival. It operates as part of the MAPK signaling pathway driven by extracellular signals. MSK1 activation occurs through ERK1/2 and p38 MAPK therefore linking it to essential cellular processes. As a kinase MSK1 phosphorylates downstream targets contributing to inflammatory responses apoptosis and gene expression modulation. MSK1 often partners with other proteins to form complexes enhancing its regulatory capabilities.

Pathways

MSK1 plays a role in both the MAPK and NF-kB pathways. These pathways contribute to controlling cell growth and immune responses. In the MAPK pathway the involvement of MSK1 influences further regulation of transcription factors. In connection with the NF-kB pathway MSK1 can regulate NF-kB activity promoting gene expression related to inflammation. Related proteins in these pathways include ERK1/2 which activate MSK1 enhancing the kinase's function in these critical cellular circuits.

MSK1 links to various conditions including cancer and neurodegenerative diseases. The dysregulation of MSK1 activity is associated with processes like uncontrolled cell proliferation and abnormal inflammation factors that contribute to tumor development and progression. In neurodegenerative diseases changes in MSK1 activity disrupt neuronal survival pathways affecting cognitive functions. MSK1 connects with proteins like CREB influencing the expression of genes relevant to these diseases' development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes (PubMed : 11909979, PubMed : 12569367, PubMed : 12763138, PubMed : 18511904, PubMed : 9687510, PubMed : 9873047). Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin (PubMed : 11909979, PubMed : 9873047). Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression (PubMed : 12628924, PubMed : 18511904). In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress (PubMed : 12628924). In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential (PubMed : 12763138). Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation (PubMed : 12569367). Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A (PubMed : 15010469). Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN (PubMed : 12773393). May also phosphorylate 'Ser-28' of histone H3 (PubMed : 12773393). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14) (PubMed : 12773393). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines (By similarity). Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors (By similarity). Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury (By similarity). Phosphorylates TRIM7 at 'Ser-107' in response to growth factor signaling via the MEK/ERK pathway, thereby stimulating its ubiquitin ligase activity (PubMed : 25851810).
See full target information RPS6KA5 phospho S376
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0325986 PubMed40526593

2025

Epac1 increases myosin regulatory light-chain phosphorylation, energetic cost of contraction, and susceptibility to heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiki Ohnuki,Kenji Suita,Misao Ishikawa,Yasumasa Mototani,Megumi Nariyama,Aiko Ito,Ichiro Matsuo,Yoshio Hayakawa,Akinaka Morii,Takao Mitsubayashi,Yasutake Saeki,Yoshihiro Ishikawa,Satoshi Okumura
View all publications
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Product promise

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