AB185471

Anti-MSMP antibody

Name: Anti-MSMP antibody (ab185471) | Abcam
Brand: Abcam Limited
SKU: AB185471
Price: 630 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal MSMP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MSMP aa 50 to C-terminus.

View Alternative Names

PSMP, MSMP, Prostate-associated microseminoprotein, PC3-secreted microprotein

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-MSMP antibody (AB185471)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MSMP antibody (AB185471)

MCF7 cells stained for MSMP (green) using ab185471 at 2 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSMP antibody (AB185471)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSMP antibody (AB185471)

Paraffin embedded human prostate tissue stained for MSMP using ab185471 at 1/200 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSMP antibody (AB185471)

Paraffin embedded human placenta tissue stained for MSMP using ab185471 at 1/200 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSMP antibody (AB185471)

Paraffin embedded human skin tissue stained for MSMP using ab185471 at 1/200 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSMP antibody (AB185471)

Paraffin embedded human cerebral cortex tissue stained for MSMP using ab185471 at 1/200 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Supplier Data

Western blot - Anti-MSMP antibody (AB185471)

All lanes:

Western blot - Anti-MSMP antibody (ab185471) at 0.4 µg/mL

Lane 1:

PC-3 cell lysate

Lane 2:

A431 cell lysate

Predicted band size: 15 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MSMP aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q1L6U9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MSMP also known as macrophage scavenger receptor-like protein serves several mechanical roles within the body. It has a molecular mass of approximately 37 kDa. MSMP is prominently expressed in prostate tissue and has been observed in other tissues such as the lung and liver. The protein influences various cellular processes through these expressions contributing to its diverse functional repertoire.

Biological function summary

MSMP interacts with the extracellular matrix and plays a role in tissue homeostasis. This protein is not known to be part of a larger complex. Its activity aids in cellular adhesion and migration impacting processes critical to development and response to environmental changes. Furthermore MSMP may influence the regulation of immune responses in its expressed regions suggesting a role in inflammatory processes.

Pathways

MSMP participates in signaling pathways that regulate cell growth and migration. It has connections to the MAPK pathway indicating a role in controlling cellular responses to different stimuli. MSMP also interacts with other proteins like fibronectin and laminin which further modulate its effects within these pathways. These interactions illustrate the protein’s involvement in complex regulatory networks.

MSMP has been implicated in prostate cancer due to its high expression in prostate cells. Abnormal expression levels can disrupt normal cell regulation contributing to tumorigenesis. Additionally MSMP may have a role in pulmonary conditions due to its presence in lung tissue. Its interaction with proteins such as interleukin-8 suggests MSMP might influence inflammatory pathways associated with respiratory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a ligand for C-C chemokine receptor CCR2 (PubMed : 24442440). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed : 24442440). Exhibits a chemotactic activity for monocytes and lymphocytes but not neutrophils (PubMed : 24442440).

See full target information MSMP

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Hormones & cancer 11:117-127 PubMed32077034

2020

The Role of mPRδ and mPRε in Human Glioblastoma Cells: Expression, Hormonal Regulation, and Possible Clinical Outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Aylin Del Moral-Morales,Juan Carlos González-Orozco,José Moisés Capetillo-Velázquez,Ana Gabriela Piña-Medina,Ignacio Camacho-Arroyo

Oncogene 37:722-731 PubMed29059175

2017

Induction of anti-VEGF therapy resistance by upregulated expression of microseminoprotein (MSMP).

Applications

Unspecified application

Species

Unspecified reactive species

T Mitamura,S Pradeep,M McGuire,S Y Wu,S Ma,H Hatakeyama,Y A Lyons,T Hisamatsu,K Noh,A Villar-Prados,X Chen,C Ivan,C Rodriguez-Aguayo,W Hu,G Lopez-Berestein,R L Coleman,A K Sood

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MSMP antibody

Immunocytochemistry/ Immunofluorescence - Anti-MSMP antibody (AB185471)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSMP antibody (AB185471)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSMP antibody (AB185471)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSMP antibody (AB185471)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSMP antibody (AB185471)

Western blot - Anti-MSMP antibody (AB185471)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise