AB245190

Anti-MSP/MST1 antibody [EPR22184-180]

RabMAb
Recombinant
KO Validated
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(4 Publications)

Rabbit Recombinant Monoclonal MSP/MST1 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications.

View Alternative Names

D3F15S2, DNF15S2, HGFL, MST1, Hepatocyte growth factor-like protein, Macrophage stimulatory protein, Macrophage-stimulating protein, MSP

3 Images

Lab

Western blot - Anti-MSP/MST1 antibody [EPR22184-180] (AB245190)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Vinculin antibody [EPR8185] - Loading Control (ab129002) staining at 1/5000 dilution.

A non-specific band has been observed by WB at approximately 110 kDa. The identify of this band is unknown.

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Mst1-KO homozygous mice (Strain ID : T037203).

All lanes:

Western blot - Anti-MSP/MST1 antibody [EPR22184-180] (ab245190) at 1/1000 dilution

Lane 1:

Wild-type mouse liver tissue lysate (male) at 40 µg

Lane 2:

Wild-type mouse liver tissue lysate (female) at 40 µg

Lane 3:

Mst1 knockout mouse liver tissue lysate (female case1) at 40 µg

Lane 4:

Mst1 knockout mouse liver tissue lysate (female case2) at 40 µg

Lane 5:

Wild-type mouse spleen tissue lysate (male) at 40 µg

Lane 6:

Wild-type mouse spleen tissue lysate (female) at 40 µg

Lane 7:

Mst1 knockout mouse spleen tissue lysate (female case1) at 40 µg

Lane 8:

Mst1 knockout mouse spleen tissue lysate (female case2) at 40 µg

Lane 9:

Mouse serum tissue lysate at 4 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 80 kDa,60 kDa,124 kDa

false

Exposure time: 180s

Unknown

Western blot - Anti-MSP/MST1 antibody [EPR22184-180] (AB245190)

Blocking/Dilution buffer : 5% NFDM/TBST.

Pro-MSP is proteolytically cleaved into alpha and beta chain. The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID : 8393443;8609228;7732008;11856768).

All lanes:

Western blot - Anti-MSP/MST1 antibody [EPR22184-180] (ab245190) at 1/1000 dilution

Lane 1:

Mouse serum at 20 µg

Lane 2:

Mouse liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 80 kDa

Observed band size: 60 kDa,80 kDa

false

Exposure time: 3min

Unknown

Western blot - Anti-MSP/MST1 antibody [EPR22184-180] (AB245190)

Blocking/Dilution buffer : 5% NFDM/TBST.

The positive band (~60-kDa) is the MSP alpha chain.

All lanes:

Western blot - Anti-MSP/MST1 antibody [EPR22184-180] (ab245190) at 1/1000 dilution

All lanes:

Mouse MSP/MST1 active protein 20 ng (aa1-716)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 80 kDa

false

Exposure time: 6s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22184-180

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

A non-specific band has been observed by WB at approximately 110 kDa. The identify of this band is unknown.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MSP/MST1 also known as macrophage stimulating protein is a serine protease family protein with a molecular mass of approximately 80 kDa. It expresses in various tissues with significant presence in liver tissue. MST1 is a precursor protein that undergoes proteolytic cleavage to become its active form. MSP protein plays an important role in signaling processes acting as a ligand for the RON receptor tyrosine kinase triggering downstream effects in various cellular systems.

Biological function summary

MSP protein contributes to numerous cellular events including migration differentiation and survival. It forms a complex with the RON receptor facilitating cell signaling cascades that influence these processes. MSP is key in the regulation of immune responses and tissue remodeling which makes it significant in inflammation and wound healing contexts. It influences macrophage activity promoting their ability to clear pathogens and repair tissues.

Pathways

MSP protein is integral to the HGF/MET signaling pathway and the related MSP/RON pathway. These pathways are involved in cellular growth motility and morphogenesis with a notable impact on epithelial cellular dynamics. MST1 through its interaction with RON influences c-Met and related pathways that intertwine with cell cycle regulation. The crosstalk with hepatocyte growth factor (HGF) can further elucidate the sophisticated regulation of cellular functions where MSP plays a role.

MSP protein has been implicated in cancer progression and inflammatory diseases. It associates closely with the progression of certain carcinomas through its interaction with the RON receptor influencing tumor proliferation and metastasis. Additionally the dysregulation of MSP is associated with chronic inflammatory conditions linking it to key pathway proteins such as HGF. The therapeutic targeting of MSP might offer insights into anticancer and anti-inflammatory treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information MST1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8160 PubMed40890143

2025

Capillary constrictions prime cancer cell tumorigenicity through PIEZO1.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Silvani,Chantal Kopecky,Sara Romanazzo,Vanina Rodríguez,Ayan Das,Elvis Pandzic,John G Lock,Christine L Chaffer,Kate Poole,Kristopher A Kilian

ESC heart failure 11:1205-1217 PubMed38288506

2024

CircRbms1 fosters MST1 mRNA and protein levels to motivate myocardial ischaemia-reperfusion injury via autophagic status.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Liu,Guorong Lai,Yanhui Hu,Fan Yang,Chao Zhang,Dongsheng Le,Fumou Deng,Xianliang Xing,Binquan Tang,Huanhuan Jie,Yingping Liang,Enjun Lei

Bioengineered 13:12726-12737 PubMed35611768

2022

Knockout of circRNA single stranded interacting protein 1 (circRBMS1) played a protective role in myocardial ischemia-reperfusion injury though inhibition of miR-2355-3p/Mammalian Sterile20-like kinase 1 (MST1) axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yingping Liang,Huanhuan Jie,Qin Liu,Chang Li,Renjie Xiao,Xianliang Xing,Jing Sun,Shuchun Yu,Yanhui Hu,Guo-Hai Xu

Frontiers in molecular biosciences 8:787008 PubMed35242811

2022

A Global Phosphorylation Atlas of Proteins Within Pathological Site of Rotator Cuff Tendinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yezhou Wang,Jiawei Zhang,Yuan Lin,Shi Cheng,Duanyang Wang,Man Rao,Yuheng Jiang,Xiang Huang,Ruijing Chen,Yong Xie,Pengbin Yin,Biao Cheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MSP/MST1 antibody [EPR22184-180]

Western blot - Anti-MSP/MST1 antibody [EPR22184-180] (AB245190)

Western blot - Anti-MSP/MST1 antibody [EPR22184-180] (AB245190)

Western blot - Anti-MSP/MST1 antibody [EPR22184-180] (AB245190)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise