Rabbit Recombinant Monoclonal MSRA antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-MSRA antibody [EPR29159-5] (AB322706), expandable thumbnail](https://content.abcam.com/products/images/msra-antibody-epr29159-5-ab322706--western-blot-img439934.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MSRA antibody [EPR29159-5] (AB322706)")
![Western blot - Anti-MSRA antibody [EPR29159-5] (AB322706), expandable thumbnail](https://content.abcam.com/products/images/msra-antibody-epr29159-5-ab322706--western-blot-img439933.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MSRA antibody [EPR29159-5] (AB322706)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.
Alternative names
Mitochondrial peptide methionine sulfoxide reductase, Peptide-methionine (S)-S-oxide reductase, Protein-methionine-S-oxide reductase, Peptide Met(O) reductase, PMSR, MSRA
Rabbit Recombinant Monoclonal MSRA antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR29159-5
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-MSRA antibody [EPR29159-5] (ab322706), expandable thumbnail](https://content.abcam.com/products/images/msra-antibody-epr29159-5-ab322706--western-blot-img439934.jpg "Western blot - Anti-MSRA antibody [EPR29159-5] (ab322706)")
Western blot - Anti-MSRA antibody [EPR29159-5] (ab322706)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: HEK-293 (PMID: 32482536), B16-F10.
This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-MSRA antibody [EPR29159-5] (ab322706) at 1/1000 dilution
Lane 1: NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg
Lane 4: B16-F10 (mouse skin melanoma cell) whole cell lysate at 20 µg
Lane 5: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 26 kDa, 36 kDa
Exposure time: 180s
![Western blot - Anti-MSRA antibody [EPR29159-5] (ab322706), expandable thumbnail](https://content.abcam.com/products/images/msra-antibody-epr29159-5-ab322706--western-blot-img439933.jpg "Western blot - Anti-MSRA antibody [EPR29159-5] (ab322706)")
Western blot - Anti-MSRA antibody [EPR29159-5] (ab322706)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: Human colon, thyroid, brainstem, and fat; Mouse skeletal muscle (PMID: 19769460) and rat skeletal muscle.
This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1-5: 114 seconds; Lane 6-9: 15 seconds
All lanes: Western blot - Anti-MSRA antibody [EPR29159-5] (ab322706) at 1/1000 dilution
Lane 1: Human liver tissue lysate at 20 µg
Lane 2: Human colon tissue lysate at 20 µg
Lane 3: Human thyroid tissue lysate at 20 µg
Lane 4: Human brainstem tissue lysate at 20 µg
Lane 5: Human fat tissue lysate at 20 µg
Lane 6: Mouse kidney tissue lysate at 20 µg
Lane 7: Mouse skeletal tissue lysate at 20 µg
Lane 8: Rat kidney tissue lysate at 20 µg
Lane 9: Rat skeletal tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 26 kDa, 36 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com