AB180584

Anti-MSRB3 antibody [EPR13664]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal MSRB3 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

UNQ1965/PRO4487, MSRB3, Methionine-R-sulfoxide reductase B3, MsrB3

1 Images

Supplier Data

Western blot - Anti-MSRB3 antibody [EPR13664] (AB180584)

All lanes:

Western blot - Anti-MSRB3 antibody [EPR13664] (ab180584) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human colon tissue lysate at 10 µg

Lane 3:

A549 cell line lysate at 10 µg

Lane 4:

HeLa cell line lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 19 kDa

false

Carrier free

Anti-MSRB3 antibody [EPR13664] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13664

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MSRB3 also known as Methionine Sulfoxide Reductase B3 is an enzyme that plays an important role in the reduction of methionine sulfoxide to methionine. This protein is about 25 kilodaltons in mass. MSRB3 is expressed in various tissues with notable presence in the liver kidneys and skin. Its cellular localization often highlights its activity and function within these tissues suggesting its role in maintaining cellular homeostasis.

Biological function summary

Methionine Sulfoxide Reductase B3 protects cells against oxidative stress by repairing oxidized methionine residues in proteins. It serves as an important player in protein repair mechanisms. MSRB3 acts independently and is not known to form parts of larger complexes. This function makes it essential for cellular resilience against oxidative damage and supports the integrity of cellular proteins under stress conditions.

Pathways

MSRB3 functions mainly within the oxidative stress response pathway where it collaborates with methionine sulfoxide reductase A (MSRA) another enzyme with a similar function. Collectively these proteins help restore oxidized methionine residues an important step in maintaining protein structure and function. MSRB3 also influences the methionine cycle which connects to broader processes such as cellular antioxidant defense.

Methionine Sulfoxide Reductase B3 has associations with hearing loss and age-related cataracts. Mutations in the MSRB3 gene can lead to a form of nonsyndromic hearing loss pointing to its significance in auditory function. The connection of MSRB3 with oxidative stress also implicates it in cataract formation where proteins like alpha-crystallins interact within the lens to maintain transparency and function. Understanding these connections provides insight into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reduction of free and protein-bound methionine sulfoxide to methionine. Isoform 2 is essential for hearing.

See full target information MSRB3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 57:387-397.e4 PubMed35134345

2022

The Lipid Droplet Knowledge Portal: A resource for systematic analyses of lipid droplet biology.

Applications

Unspecified application

Species

Unspecified reactive species

Niklas Mejhert,Katlyn R Gabriel,Scott Frendo-Cumbo,Natalie Krahmer,Jiunn Song,Leena Kuruvilla,Chandramohan Chitraju,Sebastian Boland,Dong-Keun Jang,Marcin von Grotthuss,Maria C Costanzo,Mikael Rydén,James A Olzmann,Jason Flannick,Noël P Burtt,Robert V Farese,Tobias C Walther

Genetics and molecular research : GMR 16: PubMed28407177

2017

mRNA and protein expression levels of four candidate genes for ear size in Erhualian and Large White pigs.

Applications

Unspecified application

Species

Unspecified reactive species

L C Zhang,J Liang,L Pu,Y B Zhang,L G Wang,X Liu,H Yan,L X Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MSRB3 antibody [EPR13664]

Western blot - Anti-MSRB3 antibody [EPR13664] (AB180584)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise