AB154514

Anti-MST antibody

Name: Anti-MST antibody (ab154514) | Abcam
Brand: Abcam Limited
SKU: AB154514

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(7 Publications)

Rabbit Polyclonal MST antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MPST aa 200 to C-terminus.

View Alternative Names

TST2, MPST, 3-mercaptopyruvate sulfurtransferase, MST

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (AB154514)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (AB154514)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded CL1-5 xenograft labeling MST with ab154514 at 1/100.

Unknown

Western blot - Anti-MST antibody (AB154514)

12% SDS PAGE

All lanes:

Western blot - Anti-MST antibody (ab154514) at 1/500 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 33 kDa

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Supplier Data

Western blot - Anti-MST antibody (AB154514)

12% SDS-PAGE

All lanes:

Western blot - Anti-MST antibody (ab154514) at 1/500 dilution

All lanes:

Mouse tissue extract at 50 µg

Predicted band size: 33 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human MPST aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P25325

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Alternatively Tris-EDTA buffer (pH8.0) may be used. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MST also known as Mammalian Sterile 20-like kinase or STK3 is a serine/threonine kinase with a molecular mass of about 60 kDa. MST is expressed in various tissues including the brain liver and skeletal muscle. This kinase functions mechanically by phosphorylating downstream substrates exerting control over cell proliferation apoptosis and cytoskeletal reorganization. Alternate names like 3-MST also exist which can sometimes lead to confusion regarding its precise identity among similar kinases.

Biological function summary

MST plays a significant role in the regulation of the Hippo signaling pathway particularly as a core component of the MST kinase complex. This complex interacts with several other proteins to control organ size and suppress tumor development by regulating the balance between cell division and apoptosis. MST kinases exert influence on cell cycle checkpoints and promote cellular apoptosis aiding in the maintenance of tissue homeostasis.

Pathways

MST proteins are essential within the Hippo signaling pathway and are integrally involved in modulating the MAPK pathway. MST interacts with proteins like LATS1/2 activating them to help mediate the phosphorylation of downstream effectors including YAP and TAZ in the Hippo pathway. These interactions lead to transcriptional changes that govern cell proliferation and apoptosis highlighting MST's role in ensuring proper cellular responses to external and internal stimuli.

MST dysregulation is closely linked to cancers and neurodegenerative diseases. In cancer MST's inability to regulate cell growth can result in uninhibited cell proliferation as seen in hepatocellular carcinoma where MST's interaction with YAP becomes dysfunctional. In neurodegenerative disorders particularly Alzheimer's disease pathways involving MST and related proteins such as MARK may contribute to pathological tau hyperphosphorylation leading to neuronal damage. Understanding MST's relation to these diseases aids in targeting therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.

See full target information MPST

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1013164 PubMed40388397

2025

Hydrogen sulfide (H2S) coordinates redox balance, carbon metabolism, and mitochondrial bioenergetics to suppress SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ragini Agrawal,Virender Kumar Pal,Suhas K S,Gopika Jayan Menon,Inder Raj Singh,Nitish Malhotra,Naren C S,Kailash Ganesh,Raju S Rajmani,Aswin Sai Narain Seshasayee,Nagasuma Chandra,Manjunath B Joshi,Amit Singh

Antioxidants (Basel, Switzerland) 13: PubMed39594503

2024

SOD1 Is an Integral Yet Insufficient Oxidizer of Hydrogen Sulfide in Trisomy 21 B Lymphocytes and Can Be Augmented by a Pleiotropic Carbon Nanozyme.

Applications

Unspecified application

Species

Unspecified reactive species

Karthik Mouli,Anton V Liopo,Larry J Suva,Kenneth R Olson,Emily A McHugh,James M Tour,Paul J Derry,Thomas A Kent

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400140 PubMed38973255

2024

KAT6A Condensates Impair PARP1 Trapping of PARP Inhibitors in Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyan Zhan,Jiarong Zhang,Huisheng Liang,Chong Wang,Li Hong,Wenxue Liu

Frontiers in oncology 13:1178021 PubMed37483514

2023

Cystathionine β-synthase affects organization of cytoskeleton and modulates carcinogenesis in colorectal carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Veronika Liskova,Barbora Chovancova,Petr Babula,Ingeborg Rezuchova,Kristina Ploth Pavlov,Miroslava Matuskova,Olga Krizanova

Redox biology 51:102233 PubMed35042677

2022

Overproduction of hydrogen sulfide, generated by cystathionine β-synthase, disrupts brain wave patterns and contributes to neurobehavioral dysfunction in a rat model of down syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Theodora Panagaki,Laura Lozano-Montes,Lucia Janickova,Karim Zuhra,Marcell P Szabo,Tomas Majtan,Gregor Rainer,Damien Maréchal,Yann Herault,Csaba Szabo

eLife 10: PubMed34792020

2021

Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Virender Kumar Pal,Ragini Agrawal,Srabanti Rakshit,Pooja Shekar,Diwakar Tumkur Narasimha Murthy,Annapurna Vyakarnam,Amit Singh

Biomolecules 10: PubMed33352938

2020

Role of Hydrogen Sulfide and 3-Mercaptopyruvate Sulfurtransferase in the Regulation of the Endoplasmic Reticulum Stress Response in Hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Theodora Panagaki,Elisa B Randi,Csaba Szabo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MST antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (AB154514)

Western blot - Anti-MST antibody (AB154514)

Western blot - Anti-MST antibody (AB154514)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise