Rabbit Polyclonal MST antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MPST aa 200 to C-terminus.
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Publications
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- Redox biology 51:1022332022Overproduction of hydrogen sulfide, generated by cystathionine β-synthase, disrupts brain wave patterns and contributes to neurobehavioral dysfunction in a rat model of down syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTheodora Panagaki,Laura Lozano-Montes,Lucia Janickova,Karim Zuhra,Marcell P Szabo,Tomas Majtan,Gregor Rainer,Damien Maréchal,Yann Herault,Csaba SzaboPubMed 35042677
- eLife 10:2021Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVirender Kumar Pal,Ragini Agrawal,Srabanti Rakshit,Pooja Shekar,Diwakar Tumkur Narasimha Murthy,Annapurna Vyakarnam,Amit SinghPubMed 34792020
- Biomolecules 10:2020Role of Hydrogen Sulfide and 3-Mercaptopyruvate Sulfurtransferase in the Regulation of the Endoplasmic Reticulum Stress Response in Hepatocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTheodora Panagaki et. al.PubMed 33352938
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human MPST aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P25325
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Alternatively Tris-EDTA buffer (pH8.0) may be used. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Associated Products
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Target data
Function
Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.
Alternative names
3-mercaptopyruvate sulfurtransferase, MST, TST2, MPST
Recommended products
Rabbit Polyclonal MST antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MPST aa 200 to C-terminus.
Alternative names
3-mercaptopyruvate sulfurtransferase, MST, TST2, MPST
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human MPST aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P25325
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MST also known as Mammalian Sterile 20-like kinase or STK3 is a serine/threonine kinase with a molecular mass of about 60 kDa. MST is expressed in various tissues including the brain liver and skeletal muscle. This kinase functions mechanically by phosphorylating downstream substrates exerting control over cell proliferation apoptosis and cytoskeletal reorganization. Alternate names like 3-MST also exist which can sometimes lead to confusion regarding its precise identity among similar kinases.
- Biological function summary
MST plays a significant role in the regulation of the Hippo signaling pathway particularly as a core component of the MST kinase complex. This complex interacts with several other proteins to control organ size and suppress tumor development by regulating the balance between cell division and apoptosis. MST kinases exert influence on cell cycle checkpoints and promote cellular apoptosis aiding in the maintenance of tissue homeostasis.
- Pathways
MST proteins are essential within the Hippo signaling pathway and are integrally involved in modulating the MAPK pathway. MST interacts with proteins like LATS1/2 activating them to help mediate the phosphorylation of downstream effectors including YAP and TAZ in the Hippo pathway. These interactions lead to transcriptional changes that govern cell proliferation and apoptosis highlighting MST's role in ensuring proper cellular responses to external and internal stimuli.
- Associated diseases and disorders
MST dysregulation is closely linked to cancers and neurodegenerative diseases. In cancer MST's inability to regulate cell growth can result in uninhibited cell proliferation as seen in hepatocellular carcinoma where MST's interaction with YAP becomes dysfunctional. In neurodegenerative disorders particularly Alzheimer's disease pathways involving MST and related proteins such as MARK may contribute to pathological tau hyperphosphorylation leading to neuronal damage. Understanding MST's relation to these diseases aids in targeting therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (ab154514)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded CL1-5 xenograft labeling MST with ab154514 at 1/100.
Western blot - Anti-MST antibody (ab154514)
12% SDS PAGE
All lanes: Western blot - Anti-MST antibody (ab154514) at 1/500 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 33 kDa
Western blot - Anti-MST antibody (ab154514)
12% SDS-PAGE
All lanes: Western blot - Anti-MST antibody (ab154514) at 1/500 dilution
All lanes: Mouse tissue extract at 50 µg
Predicted band size: 33 kDa
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