Rabbit Polyclonal MST antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MPST aa 100 to C-terminus.
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Publications
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- Journal of medical virology 95:e281762022Hydrogen sulfide inhibits human T-cell leukemia virus type-1 (HTLV-1) protein expression via regulation of ATG4B.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuandi Liu,Jiaxiang Sun,Xuhong Cheng,Liangwei Duan,Shuaifeng Guo,Zhongxin Zhang,Jia Wan,Chunduo Wang,Xiaoying Zhi,Linghui Yuan,Hui WangPubMed 36163615
- Redox biology 56:1024662022Regulation of CyR61 expression and release by 3-mercaptopyruvate sulfurtransferase in colon cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKelly Ascenção,Bassma Lheimeur,Csaba SzaboPubMed 36113340
- Frontiers in pharmacology 12:6903712021Hydrogen Sulfide Ameliorates Angiotensin II-Induced Atrial Fibrosis Progression to Atrial Fibrillation Through Inhibition of the Warburg Effect and Endoplasmic Reticulum Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeng-Jing Hu,Xiu-Heng Wang,Yao Liu,Tian-Qing Zhang,Zheng-Rong Chen,Chi Zhang,Zhi-Han Tang,Shun-Lin Qu,Hui-Fang Tang,Zhi-Sheng JiangPubMed 34950023
- Cell reports 37:1099582021The hepatic compensatory response to elevated systemic sulfide promotes diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoderick N Carter,Matthew T G Gibbins,Martin E Barrios-Llerena,Stephen E Wilkie,Peter L Freddolino,Marouane Libiad,Victor Vitvitsky,Barry Emerson,Thierry Le Bihan,Madara Brice,Huizhong Su,Scott G Denham,Natalie Z M Homer,Clare Mc Fadden,Anne Tailleux,Nourdine Faresse,Thierry Sulpice,Francois Briand,Tom Gillingwater,Kyo Han Ahn,Subhankar Singha,Claire McMaster,Richard C Hartley,Bart Staels,Gillian A Gray,Andrew J Finch,Colin Selman,Ruma Banerjee,Nicholas M MortonPubMed 34758301
- Medical science monitor : international medical journal of experimental and clinical research 27:e9293892021Association of Hydrogen Sulfide with Femoral Bone Mineral Density in Osteoporosis Patients: A Preliminary Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan-Ming Hao,Da-Wei He,Yan Gao,Ling-Na Fang,Pan-Pan Zhang,Ke Lu,Rong-Zhu Lu,Chong LiPubMed 33714972
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MPST aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P25325
Reactivity data
Select an application| IHC-P | ICC | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Fixation/Permeabilization: PFA/Triton X-100. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.
Alternative names
TST2, MPST, 3-mercaptopyruvate sulfurtransferase, MST
Recommended products
Rabbit Polyclonal MST antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MPST aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MPST aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P25325
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MST also known as Mammalian Sterile 20-like kinase or STK3 is a serine/threonine kinase with a molecular mass of about 60 kDa. MST is expressed in various tissues including the brain liver and skeletal muscle. This kinase functions mechanically by phosphorylating downstream substrates exerting control over cell proliferation apoptosis and cytoskeletal reorganization. Alternate names like 3-MST also exist which can sometimes lead to confusion regarding its precise identity among similar kinases.
- Biological function summary
MST plays a significant role in the regulation of the Hippo signaling pathway particularly as a core component of the MST kinase complex. This complex interacts with several other proteins to control organ size and suppress tumor development by regulating the balance between cell division and apoptosis. MST kinases exert influence on cell cycle checkpoints and promote cellular apoptosis aiding in the maintenance of tissue homeostasis.
- Pathways
MST proteins are essential within the Hippo signaling pathway and are integrally involved in modulating the MAPK pathway. MST interacts with proteins like LATS1/2 activating them to help mediate the phosphorylation of downstream effectors including YAP and TAZ in the Hippo pathway. These interactions lead to transcriptional changes that govern cell proliferation and apoptosis highlighting MST's role in ensuring proper cellular responses to external and internal stimuli.
- Associated diseases and disorders
MST dysregulation is closely linked to cancers and neurodegenerative diseases. In cancer MST's inability to regulate cell growth can result in uninhibited cell proliferation as seen in hepatocellular carcinoma where MST's interaction with YAP becomes dysfunctional. In neurodegenerative disorders particularly Alzheimer's disease pathways involving MST and related proteins such as MARK may contribute to pathological tau hyperphosphorylation leading to neuronal damage. Understanding MST's relation to these diseases aids in targeting therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-MST antibody (ab224043)
Western blot analysis labeling MST with ab224043 at 0.04 ug/ml.
All lanes: Western blot - Anti-MST antibody (ab224043) at 0.04 µg/mL
Lane 1: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2: U-251 MG (Human brain glioma cell line) whole cell lysate
Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (ab224043)
Paraffin-embedded human liver tissue stained for MST using ab224043 at 1/1000 dilution in immunohistochemical analysis.
Immunocytochemistry - Anti-MST antibody (ab224043)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for MST (green) using ab224043 at 4 μg/ml in ICC.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (ab224043)
Paraffin-embedded human kidney tissue stained for MST using ab224043 at 1/1000 dilution in immunohistochemical analysis.
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