AB224043

Anti-MST antibody

Name: Anti-MST antibody (ab224043) | Abcam
Brand: Abcam Limited
SKU: AB224043
Price: 545 USD
Availability: InStock

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(9 Publications)

Rabbit Polyclonal MST antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MPST aa 100 to C-terminus.

View Alternative Names

TST2, MPST, 3-mercaptopyruvate sulfurtransferase, MST

4 Images

Supplier Data

Immunocytochemistry - Anti-MST antibody (AB224043)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for MST (green) using ab224043 at 4 μg/ml in ICC.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (AB224043)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (AB224043)

Paraffin-embedded human kidney tissue stained for MST using ab224043 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (AB224043)

Paraffin-embedded human liver tissue stained for MST using ab224043 at 1/1000 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-MST antibody (AB224043)

Western blot analysis labeling MST with ab224043 at 0.04 ug/ml.

All lanes:

Western blot - Anti-MST antibody (ab224043) at 0.04 µg/mL

Lane 1:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

Lane 2:

U-251 MG (Human brain glioma cell line) whole cell lysate

Predicted band size: 33 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC

applications

Immunogen

Recombinant Fragment Protein within Human MPST aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P25325

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "Fixation/Permeabilization: PFA/Triton X-100.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MST also known as Mammalian Sterile 20-like kinase or STK3 is a serine/threonine kinase with a molecular mass of about 60 kDa. MST is expressed in various tissues including the brain liver and skeletal muscle. This kinase functions mechanically by phosphorylating downstream substrates exerting control over cell proliferation apoptosis and cytoskeletal reorganization. Alternate names like 3-MST also exist which can sometimes lead to confusion regarding its precise identity among similar kinases.

Biological function summary

MST plays a significant role in the regulation of the Hippo signaling pathway particularly as a core component of the MST kinase complex. This complex interacts with several other proteins to control organ size and suppress tumor development by regulating the balance between cell division and apoptosis. MST kinases exert influence on cell cycle checkpoints and promote cellular apoptosis aiding in the maintenance of tissue homeostasis.

Pathways

MST proteins are essential within the Hippo signaling pathway and are integrally involved in modulating the MAPK pathway. MST interacts with proteins like LATS1/2 activating them to help mediate the phosphorylation of downstream effectors including YAP and TAZ in the Hippo pathway. These interactions lead to transcriptional changes that govern cell proliferation and apoptosis highlighting MST's role in ensuring proper cellular responses to external and internal stimuli.

MST dysregulation is closely linked to cancers and neurodegenerative diseases. In cancer MST's inability to regulate cell growth can result in uninhibited cell proliferation as seen in hepatocellular carcinoma where MST's interaction with YAP becomes dysfunctional. In neurodegenerative disorders particularly Alzheimer's disease pathways involving MST and related proteins such as MARK may contribute to pathological tau hyperphosphorylation leading to neuronal damage. Understanding MST's relation to these diseases aids in targeting therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.

See full target information MPST

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Pathophysiology : the official journal of the International Society for Pathophysiology 32: PubMed40137467

2025

Altered Production and Cellular Levels of Hydrogen Sulfide (HS) in Placental Trophoblasts from Pregnancies Affected by Pre-Eclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodan Chu,Jie Xu,Xinggui Shen,Wenji Sheng,Jingxia Sun,Yang Gu,David F Lewis,Danielle Cooper,Dani Zoorob,Yuping Wang

Redox biology 68:102965 PubMed38000344

2023

Thiosulfate sulfurtransferase deficiency promotes oxidative distress and aberrant NRF2 function in the brain.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Luo,Laurent Chatre,Shaden Melhem,Zayana M Al-Dahmani,Natalie Z M Homer,Anneke Miedema,Leo E Deelman,Matthew R Groves,Martin Feelisch,Nicholas M Morton,Amalia Dolga,Harry van Goor

Journal of medical virology 95:e28176 PubMed36163615

2022

Hydrogen sulfide inhibits human T-cell leukemia virus type-1 (HTLV-1) protein expression via regulation of ATG4B.

Applications

Unspecified application

Species

Unspecified reactive species

Huandi Liu,Jiaxiang Sun,Xuhong Cheng,Liangwei Duan,Shuaifeng Guo,Zhongxin Zhang,Jia Wan,Chunduo Wang,Xiaoying Zhi,Linghui Yuan,Hui Wang

Antioxidants (Basel, Switzerland) 11: PubMed36139896

2022

Sequential Accumulation of 'Driver' Pathway Mutations Induces the Upregulation of Hydrogen-Sulfide-Producing Enzymes in Human Colonic Epithelial Cell Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly Ascenção,Nahzli Dilek,Karim Zuhra,Katalin Módis,Toshiro Sato,Csaba Szabo

Redox biology 56:102466 PubMed36113340

2022

Regulation of CyR61 expression and release by 3-mercaptopyruvate sulfurtransferase in colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly Ascenção,Bassma Lheimeur,Csaba Szabo

Frontiers in pharmacology 12:690371 PubMed34950023

2021

Hydrogen Sulfide Ameliorates Angiotensin II-Induced Atrial Fibrosis Progression to Atrial Fibrillation Through Inhibition of the Warburg Effect and Endoplasmic Reticulum Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Heng-Jing Hu,Xiu-Heng Wang,Yao Liu,Tian-Qing Zhang,Zheng-Rong Chen,Chi Zhang,Zhi-Han Tang,Shun-Lin Qu,Hui-Fang Tang,Zhi-Sheng Jiang

Cell reports 37:109958 PubMed34758301

2021

The hepatic compensatory response to elevated systemic sulfide promotes diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Roderick N Carter,Matthew T G Gibbins,Martin E Barrios-Llerena,Stephen E Wilkie,Peter L Freddolino,Marouane Libiad,Victor Vitvitsky,Barry Emerson,Thierry Le Bihan,Madara Brice,Huizhong Su,Scott G Denham,Natalie Z M Homer,Clare Mc Fadden,Anne Tailleux,Nourdine Faresse,Thierry Sulpice,Francois Briand,Tom Gillingwater,Kyo Han Ahn,Subhankar Singha,Claire McMaster,Richard C Hartley,Bart Staels,Gillian A Gray,Andrew J Finch,Colin Selman,Ruma Banerjee,Nicholas M Morton

Journal of neuroscience methods 361:109285 PubMed34242703

2021

Protein-retention expansion microscopy for visualizing subcellular organelles in fixed brain tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Logan A Campbell,Katy E Pannoni,Niesha A Savory,Dinesh Lal,Shannon Farris

Medical science monitor : international medical journal of experimental and clinical research 27:e929389 PubMed33714972

2021

Association of Hydrogen Sulfide with Femoral Bone Mineral Density in Osteoporosis Patients: A Preliminary Study.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ming Hao,Da-Wei He,Yan Gao,Ling-Na Fang,Pan-Pan Zhang,Ke Lu,Rong-Zhu Lu,Chong Li

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MST antibody

Immunocytochemistry - Anti-MST antibody (AB224043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (AB224043)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (AB224043)

Western blot - Anti-MST antibody (AB224043)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise