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Rabbit Polyclonal MST antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MPST aa 100 to C-terminus.

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Images

Western blot - Anti-MST antibody (AB224043), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (AB224043), expandable thumbnail
  • Immunocytochemistry - Anti-MST antibody (AB224043), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (AB224043), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human MPST aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P25325

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICCWB
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000.00000 - 1/2500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Tested
Tested

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Product

Target data

Function

Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.

Alternative names

Recommended products

Rabbit Polyclonal MST antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MPST aa 100 to C-terminus.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human MPST aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P25325
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MST also known as Mammalian Sterile 20-like kinase or STK3 is a serine/threonine kinase with a molecular mass of about 60 kDa. MST is expressed in various tissues including the brain liver and skeletal muscle. This kinase functions mechanically by phosphorylating downstream substrates exerting control over cell proliferation apoptosis and cytoskeletal reorganization. Alternate names like 3-MST also exist which can sometimes lead to confusion regarding its precise identity among similar kinases.

Biological function summary

MST plays a significant role in the regulation of the Hippo signaling pathway particularly as a core component of the MST kinase complex. This complex interacts with several other proteins to control organ size and suppress tumor development by regulating the balance between cell division and apoptosis. MST kinases exert influence on cell cycle checkpoints and promote cellular apoptosis aiding in the maintenance of tissue homeostasis.

Pathways

MST proteins are essential within the Hippo signaling pathway and are integrally involved in modulating the MAPK pathway. MST interacts with proteins like LATS1/2 activating them to help mediate the phosphorylation of downstream effectors including YAP and TAZ in the Hippo pathway. These interactions lead to transcriptional changes that govern cell proliferation and apoptosis highlighting MST's role in ensuring proper cellular responses to external and internal stimuli.

Associated diseases and disorders

MST dysregulation is closely linked to cancers and neurodegenerative diseases. In cancer MST's inability to regulate cell growth can result in uninhibited cell proliferation as seen in hepatocellular carcinoma where MST's interaction with YAP becomes dysfunctional. In neurodegenerative disorders particularly Alzheimer's disease pathways involving MST and related proteins such as MARK may contribute to pathological tau hyperphosphorylation leading to neuronal damage. Understanding MST's relation to these diseases aids in targeting therapeutic interventions.

Product promise

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4 product images

  • Western blot - Anti-MST antibody (ab224043), expandable thumbnail

    Western blot - Anti-MST antibody (ab224043)

    Western blot analysis labeling MST with ab224043 at 0.04 ug/ml.

    All lanes: Western blot - Anti-MST antibody (ab224043) at 0.04 µg/mL

    Lane 1: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

    Lane 2: U-251 MG (Human brain glioma cell line) whole cell lysate

    Predicted band size: 33 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (ab224043), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (ab224043)

    Paraffin-embedded human liver tissue stained for MST using ab224043 at 1/1000 dilution in immunohistochemical analysis.

  • Immunocytochemistry - Anti-MST antibody (ab224043), expandable thumbnail

    Immunocytochemistry - Anti-MST antibody (ab224043)

    PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for MST (green) using ab224043 at 4 μg/ml in ICC.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (ab224043), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST antibody (ab224043)

    Paraffin-embedded human kidney tissue stained for MST using ab224043 at 1/1000 dilution in immunohistochemical analysis.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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