AB87322

Anti-MST1/MST2 antibody

Name: Anti-MST1/MST2 antibody (ab87322) | Abcam
Brand: Abcam Limited
SKU: AB87322
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal STK3/MST-2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human STK3.

View Alternative Names

KRS1, MST2, STK3, Serine/threonine-protein kinase 3, Mammalian STE20-like protein kinase 2, STE20-like kinase MST2, Serine/threonine-protein kinase Krs-1, MST-2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST1/MST2 antibody (AB87322)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST1/MST2 antibody (AB87322)

Detection of Human MST1/2 by immunohistochemistry on formalin-fixed, paraffin-embedded Human colon carcinoma tissue, using ab87322 at a dilution of 1/250.
Detection : DAB staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST1/MST2 antibody (AB87322)

Detection of Human MST1/2 by immunohistochemistry on formalin-fixed, paraffin-embedded Human placenta tissue using ab87322 at a dilution of 1/250.
Detection : DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human STK3. The exact immunogen used to generate this antibody is proprietary information.

Q13188

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Tilapia": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MST1/MST2 proteins also known as STK4 and STK3 play mechanical roles as serine/threonine kinases. Each protein has a molecular mass of around 56-58 kDa. They are expressed in many tissues with high levels noted in the liver pancreas and kidney. These proteins exist mainly in the cytoplasm and can translocate to the nucleus upon activation. Activation usually takes place through autophosphorylation at certain residues which further facilitates phosphorylation of downstream substrates.

Biological function summary

MST1/MST2 function as core components of the Hippo signaling pathway. They act in a kinase complex together with other proteins like SAV1 LATS1 and MOB1. Their activities involve regulating cell proliferation apoptosis and organ size control. By phosphorylating other signaling molecules they decide whether cells enter apoptosis or continue proliferating impacting cellular homeostasis. MST1/MST2 phosphorylation triggers a cascade that ultimately influences transcriptional control in the nucleus.

Pathways

Both MST1 and MST2 integrate into the Hippo and Ras signaling pathways. Within the Hippo pathway they phosphorylate large tumor suppressors such as LATS1 and LATS2 which then regulate the transcriptional co-activators YAP and TAZ. This interaction helps determine gene expression associated with cell growth and survival. Hippo pathway's role links with Ras signaling where unchecked activity can lead to oncogenesis. MST1/MST2 function connects with MAP kinase proteins that play parts in oncogenic and apoptotic balance.

MST1 and MST2 exhibit connections to cancer and neurodegenerative diseases. Their regulatory dysfunction can contribute to tumor development due to disrupted cellular homeostasis. Low expression or mutation in MST genes relates to multiple types of cancers including hepatocellular carcinoma. MST1 has an association with neurodegenerative conditions like Alzheimer's disease where its role involves apoptosis-related protein interactions including those with caspases. Proper MST1/MST2 function is essential for maintaining controlled cell growth and apoptosis to prevent such diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation (PubMed : 11278283, PubMed : 8566796, PubMed : 8816758). Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed : 15688006, PubMed : 16930133, PubMed : 23972470, PubMed : 28087714, PubMed : 29063833, PubMed : 30622739). Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed : 15688006, PubMed : 16930133, PubMed : 23972470, PubMed : 28087714). STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250 (PubMed : 21076410, PubMed : 21723128). In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation (PubMed : 21104395). Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259' (PubMed : 20212043). Phosphorylates MOBKL1A and RASSF2 (PubMed : 19525978). Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38 (PubMed : 18328708, PubMed : 18362890).

See full target information STK3

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Diagnostics (Basel, Switzerland) 12: PubMed36552980

2022

Expression of the Hippo Pathway Core Components in Endometrial Cancer and Its Association with Clinicopathologic Features.

Applications

Unspecified application

Species

Unspecified reactive species

Juseok Yang,Dae Hyun Song,Cho Hee Kim,Min Hye Kim,Hyen Chul Jo,Hyoeun Kim,Ji Eun Park,Jong Chul Baek

Medicine 100:e27032 PubMed34449481

2021

Expression of mammalian sterile 20-like kinase 1 and 2 and Yes-associated protein 1 proteins in triple-negative breast cancer and the clinicopathological significance.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Feng,Hongfei Ci,Qiong Wu

Journal of oncology 2021:8842554 PubMed33519935

2021

Evaluation of Hippo Pathway and CD133 in Radiation Resistance in Small-Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kui Yang,Yang Zhao,Yonghao Du,Ruixiang Tang

Journal of virology 93: PubMed31434729

2019

Transcriptional Response of Ovine Lung to Infection with Jaagsiekte Sheep Retrovirus.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Eleonora Karagianni,Deepali Vasoya,Jeanie Finlayson,Henny M Martineau,Ann R Wood,Chris Cousens,Mark P Dagleish,Mick Watson,David J Griffiths

Developmental cell 43:48-59.e5 PubMed29017029

2017

Fgf10-Hippo Epithelial-Mesenchymal Crosstalk Maintains and Recruits Lung Basal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Volckaert,Tingting Yuan,Cho-Ming Chao,Harold Bell,Alina Sitaula,Luisa Szimmtenings,Elie El Agha,Diptiman Chanda,Susan Majka,Saverio Bellusci,Victor J Thannickal,Reinhard Fässler,Stijn P De Langhe

Molecular and cellular biology 32:5116-28 PubMed23071096

2012

Hippo signaling regulates pancreas development through inactivation of Yap.

Applications

IHC-P

Species

Mouse

Nicholas M George,Caroline E Day,Brian P Boerner,Randy L Johnson,Nora E Sarvetnick

View all publications

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Product promise

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Anti-MST1/MST2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST1/MST2 antibody (AB87322)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST1/MST2 antibody (AB87322)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise