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Rabbit Recombinant Monoclonal MST3 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 9 publications.


Images

Western blot - Anti-MST3 antibody [EP1468Y] (AB51137), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST3 antibody [EP1468Y] (AB51137), expandable thumbnail
  • Western blot - Anti-MST3 antibody [EP1468Y] (AB51137), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MST3 antibody [EP1468Y] (AB51137), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MST3 antibody [EP1468Y] (AB51137), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. In association with STK26 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation (PubMed:27807006). Regulates also cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed:18782753).

Alternative names

MST3, STK3, STK24, Serine/threonine-protein kinase 24, Mammalian STE20-like protein kinase 3, STE20-like kinase MST3, MST-3

Recommended products

Rabbit Recombinant Monoclonal MST3 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 9 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1468Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Mammalian Sterile 20-like kinase 3 also known as MST3 or STK24 is a serine/threonine protein kinase with a mass of approximately 49 kDa. MST3 belongs to the STE20 group of kinases which play roles in various cellular processes. It localizes primarily in the cytoplasm and nucleus and sees significant expression in the brain heart liver and skeletal muscles. MST3 also associates with cellular structures such as the Golgi apparatus suggesting a role in intracellular transport or signaling.

Biological function summary

MST3 participates in regulating cell proliferation apoptosis and migration. It is not part of a larger complex but works closely with other signaling proteins to influence these processes. MST3 impacts cytoskeletal organization contributing to the dynamic remodeling of actin filaments. This protein also affects the apoptotic pathways by modulating the interactions with other kinases and apoptosis-related factors thereby aiding in the maintenance of cellular homeostasis.

Pathways

MST3 plays significant roles in the Hippo signaling pathway and the MAPK pathway—both critical for cell growth survival and differentiation. MST3 shares pathways with kinases like MOB1 and NDR1/2 in the Hippo pathway where it assists in regulating cell proliferation and apoptosis. In the context of MAPK signaling MST3 interacts with and is regulated by RAF1 and MEK1/2 coordinating cell stress responses and signaling cascades.

Associated diseases and disorders

MST3 has associations with neurological disorders and cancer. In the context of neurological disorders MST3 activity affects neuronal apoptosis and tau phosphorylation processes that relate to neurodegenerative diseases. In cancer biology MST3 regulates apoptosis and proliferation and its dysregulation might contribute to tumorigenesis. MST3 connects with proteins such as BCL2 and PARP1 that also influence these disease states making it a protein of interest for therapeutic research.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-MST3 antibody [EP1468Y] (ab51137), expandable thumbnail

    Western blot - Anti-MST3 antibody [EP1468Y] (ab51137)

    Lanes 1 - 4: Merged signal (red and green). Green - ab51137 observed at 52 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab51137 was shown to react with MST3 in wild-type A431 cells in Western blot with loss of signal observed in STK24 knockout cell line Human STK24 knockout A-431 cell line ab269479 (knockout cell lysate Human STK24 knockout A-431 cell lysate ab269643). Wild-type A431 and STK24 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab51137 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-MST3 antibody [EP1468Y] (ab51137) at 1/1000 dilution

    Lane 1: Wild-type A431 cell lysate at 20 µg

    Lane 2: STK24 knockout A431 cell lysate at 20 µg

    Lane 2: Western blot - Human STK24 knockout A-431 cell line (Human STK24 knockout A-431 cell line ab269479)

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: Raji cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 49 kDa

    Observed band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST3 antibody [EP1468Y] (ab51137), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST3 antibody [EP1468Y] (ab51137)

    ab51137 (1:100) staining human MST3 in human brain tissue by immunohistochemistry using paraffin embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-MST3 antibody [EP1468Y] (ab51137), expandable thumbnail

    Western blot - Anti-MST3 antibody [EP1468Y] (ab51137)

    All lanes: Western blot - Anti-MST3 antibody [EP1468Y] (ab51137) at 1/5000 dilution

    All lanes: HeLa cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 49 kDa

    Observed band size: 52 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MST3 antibody [EP1468Y] (ab51137), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MST3 antibody [EP1468Y] (ab51137)

    Immunofluorescent staining of HeLa cells using ab51137 (1:100).

  • Flow Cytometry (Intracellular) - Anti-MST3 antibody [EP1468Y] (ab51137), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MST3 antibody [EP1468Y] (ab51137)

    Overlay histogram showing HeLa cells stained with ab51137 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51137, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

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Product protocols

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