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AB249938

Anti-MST4 antibody [EPR12766] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MST4 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

MASK, MST4, STK26, Serine/threonine-protein kinase 26, MST3 and SOK1-related kinase, Mammalian STE20-like protein kinase 4, Serine/threonine-protein kinase MASK, MST-4, STE20-like kinase MST4

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST4 antibody [EPR12766] - BSA and Azide free (AB249938)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST4 antibody [EPR12766] - BSA and Azide free (AB249938)

This data was developed using ab177153, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling MST4 with ab177153 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST4 antibody [EPR12766] - BSA and Azide free (AB249938)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MST4 antibody [EPR12766] - BSA and Azide free (AB249938)

This data was developed using ab177153, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling MST4 with ab177153 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-MST4 antibody [EPR12766] - BSA and Azide free (AB249938)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MST4 antibody [EPR12766] - BSA and Azide free (AB249938)

This data was developed using ab177153, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling MST4 with ab177153 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

Western blot - Anti-MST4 antibody [EPR12766] - BSA and Azide free (AB249938)
  • WB

Supplier Data

Western blot - Anti-MST4 antibody [EPR12766] - BSA and Azide free (AB249938)

This data was developed using ab177153, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MST4 antibody [EPR12766] (<a href='/en-us/products/primary-antibodies/mst4-antibody-epr12766-ab177153'>ab177153</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

Human fetal brain tissue lysate at 10 µg

Lane 5:

PC-12 cell lysate at 10 µg

Lane 6:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12766

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249938 is the carrier-free version of ab177153.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MST4 also known as mammalian sterile 20-like kinase 4 is a serine/threonine kinase with a molecular mass of approximately 53 kDa. It belongs to the germinal center kinase (GCK) family and is expressed in various tissues showing higher levels in the brain and testis. MST4 localizes to the Golgi apparatus where it plays a role in regulating cellular processes.
Biological function summary

MST4 contributes to cell growth apoptosis and stress response signaling. It does not operate alone; MST4 is a part of the STRIPAK complex. The protein involves itself in the regulation of the actin cytoskeleton thereby influencing cell motility and polarity. By modulating these processes MST4 can impact developmental and stress-induced cellular pathways.

Pathways

MST4 participates in the Hippo signaling pathway which regulates organ size by controlling cell proliferation and apoptosis. In this pathway MST4 interacts with other proteins such as MST1 and MST2 to execute its functions. MST4 also participates in the MAPK signaling pathway where it connects to various cellular stress responses contributing to the transcriptional activity of downstream effectors.

MST4 has implications in cancer and neurodegenerative diseases. Alterations in MST4 expression or activity have been associated with tumor progression in several cancers. Additionally it's linked to neurodegeneration through its interactions with proteins such as Tau where dysregulated pathways may lead to pathological conditions. These connections highlight the importance of MST4 in maintaining cellular homeostasis and the implications of its dysfunction in disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase that acts as a mediator of cell growth (PubMed : 11641781, PubMed : 17360971). Modulates apoptosis (PubMed : 11641781, PubMed : 17360971). In association with STK24 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation (PubMed : 27807006). Phosphorylates ATG4B at 'Ser-383', thereby increasing autophagic flux (PubMed : 29232556). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed : 18782753).
See full target information STK26
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 7:357 PubMed34785642

2021

UBE2S promotes cell chemoresistance through PTEN-AKT signaling in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Gui,Sicai Zhang,Yongzi Xu,Hongwei Zhang,Ying Zhu,Lianbao Kong
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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