AB76579

Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y]

RabMAb
Recombinant
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(10 Publications)

Rabbit Recombinant Monoclonal STK25 phospho T174 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications.

View Alternative Names

SOK1, YSK1, STK25, Serine/threonine-protein kinase 25, Ste20-like kinase, Sterile 20/oxidant stress-response kinase 1, SOK-1, Ste20/oxidant stress response kinase 1, MST3, STK3, STK24, Serine/threonine-protein kinase 24, Mammalian STE20-like protein kinase 3, STE20-like kinase MST3, MST-3, MASK, MST4, STK26, Serine/threonine-protein kinase 26, MST3 and SOK1-related kinase, Mammalian STE20-like protein kinase 4, Serine/threonine-protein kinase MASK, MST-4, STE20-like kinase MST4

1 Images

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Western blot - Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (AB76579)

All lanes:

Western blot - Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (ab76579) at 1/2000 dilution

Lane 1:

HeLa cell lysates, untreated at 10 µg

Lane 2:

HeLa cell lysates, treated with calyculin A at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 47-49 kDa

Observed band size: 52 kDa

false

Carrier free

Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2123Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein kinases MST4 MST3 and STK25 are part of the Ste20 kinase family. MST4 and MST3 are often referred to as serine/threonine-protein kinases 26 and 24 respectively while STK25 is known as Ste20-like kinase. These proteins share a common modular architecture consisting of a kinase domain and various regulatory regions. The molecular masses are approximately 50 kDa for MST4 and MST3 and around 54 kDa for STK25. You can find these proteins expressed in multiple tissues including brain testis and spleen.

Biological function summary

MST4 MST3 and STK25 regulate cell growth proliferation and apoptosis. These proteins interact with various cellular components to form signaling complexes that influence cellular responses. MST3 and MST4 can activate apoptosis signal-regulating kinase 1 (ASK1) which leads to the apoptosis pathway. STK25 plays a role in the Golgi apparatus' functionality affecting cellular trafficking and signal transduction.

Pathways

MST4 MST3 and STK25 are involved in critical signaling pathways like the Hippo pathway and MAPK signaling. Within these pathways MST4 and MST3 together associate with MOB1 and LATS1/2 enhancing their functions. STK25 interacts with STRAD and LKB1 contributing to cell polarity regulation and cellular response to external stimuli.

Altered expression and mutations in MST4 MST3 and STK25 associate with cancer and neurological disorders. Cancer progression links strongly with these kinases' dysregulation due to their role in apoptosis and cell proliferation where MST4 activates CST complex involved in these diseases. Neurodegenerative disorders involve STK25 connected via its regulatory influence on cellular structures and its interactions with Huntington protein influencing disease pathophysiology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed : 18782753).

See full target information STK25 phospho T174

Additional targets

STK26 phospho T178,STK24 phospho T190

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 8: PubMed40639948

2025

TLR-induced STK25 activation promotes IRF5-mediated inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew R Rice,Bharati Matta,Loretta Wang,Qi Luo,Jeremy De Guzman,Dinesh Srinivasan,Katelyn R Ludwig,Surya Indukuri,Leianna Brune,Seng-Lai Tan,Betsy J Barnes

Nature communications 16:1406 PubMed39915446

2025

ROS-induced cytosolic release of mitochondrial PGAM5 promotes colorectal cancer progression by interacting with MST3.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyang Wang,Xi Wu,Wenxin Bi,Jiuzhi Xu,Liyuan Hou,Guilin Li,Yuwei Pan,Hanfu Zhang,Mengzhen Li,Sujuan Du,Mingxin Zhang,Di Liu,Shuiling Jin,Xiaojing Shi,Yuhua Tian,Jianwei Shuai,Maksim V Plikus,Moshi Song,Zhaocai Zhou,Lu Yu,Cong Lv,Zhengquan Yu

The Journal of biological chemistry 300:107255 PubMed38579991

2024

Mst4, a novel cardiac STRIPAK complex-associated kinase, regulates cardiomyocyte growth and survival and is upregulated in human cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias Eden,Marius Leye,Justus Hahn,Emanuel Heilein,Marcin Luzarowski,Bill Völschow,Christin Tannert,Samuel Sossalla,Carlota Lucena-Porcel,Derk Frank,Norbert Frey

International journal of molecular sciences 22: PubMed34062838

2021

Cellular Mechanisms of the Anti-Arrhythmic Effect of Cardiac PDE2 Overexpression.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Wagner,Mirna S Sadek,Nataliya Dybkova,Fleur E Mason,Johann Klehr,Rebecca Firneburg,Eleder Cachorro,Kurt Richter,Erik Klapproth,Stephan R Kuenzel,Kristina Lorenz,Jordi Heijman,Dobromir Dobrev,Ali El-Armouche,Samuel Sossalla,Susanne Kämmerer

The Journal of experimental medicine 217: PubMed32271880

2020

MST4 kinase suppresses gastric tumorigenesis by limiting YAP activation via a non-canonical pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liwei An,Pingping Nie,Min Chen,Yang Tang,Hui Zhang,Jingmin Guan,Zhifa Cao,Chun Hou,Wenjia Wang,Yun Zhao,Huixiong Xu,Shi Jiao,Zhaocai Zhou

Frontiers in cell and developmental biology 8:146 PubMed32258031

2020

The STRIPAK Complex Regulates Response to Chemotherapy Through p21 and p27.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Rodriguez-Cupello,Monica Dam,Laura Serini,Shan Wang,David Lindgren,Emelie Englund,Pontus Kjellman,Håkan Axelson,Alberto García-Mariscal,Chris D Madsen

Journal of cell science 129:4466-4479 PubMed27807006

2016

RHO binding to FAM65A regulates Golgi reorientation during cell migration.

Applications

Species

Human

Faraz K Mardakheh,Annette Self,Christopher J Marshall

The Journal of biological chemistry 287:29285-9 PubMed22782892

2012

STK25 protein mediates TrkA and CCM2 protein-dependent death in pediatric tumor cells of neural origin.

Applications

Species

Human

Barbara Costa,Michelle J Kean,Volker Ast,James D R Knight,Alice Mett,Zehava Levy,Derek F Ceccarelli,Beatriz Gonzalez Badillo,Roland Eils,Rainer König,Anne-Claude Gingras,Mike Fainzilber

BMC biochemistry 12:54 PubMed21985334

2011

Protein phosphatase 2a (PP2A) binds within the oligomerization domain of striatin and regulates the phosphorylation and activation of the mammalian Ste20-Like kinase Mst3.

Applications

Species

Unspecified reactive species

Johnthan Gordon,Juyeon Hwang,Karma J Carrier,Candace A Jones,Quiana L Kern,Carlos S Moreno,Richard H Karas,David C Pallas

Molecular and cellular biology 31:1382-95 PubMed21262772

2011

Human NDR kinases control G(1)/S cell cycle transition by directly regulating p21 stability.

Applications

Unspecified application

Species

Unspecified reactive species

Hauke Cornils,Reto S Kohler,Alexander Hergovich,Brian A Hemmings

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y]

Western blot - Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (AB76579)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (10)

Product promise