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Rabbit Recombinant Monoclonal STK25 phospho T174 antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] - BSA and Azide free (AB247489), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed:18782753).

Additional Targets

STK26 phospho T178, STK24 phospho T190

Alternative names

SOK1, YSK1, STK25, Serine/threonine-protein kinase 25, Ste20-like kinase, Sterile 20/oxidant stress-response kinase 1, SOK-1, Ste20/oxidant stress response kinase 1

Recommended products

Rabbit Recombinant Monoclonal STK25 phospho T174 antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP2123Y
Purification technique
Affinity purification Protein A
Concentration
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Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247489 is the carrier-free version of Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] ab76579.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein kinases MST4 MST3 and STK25 are part of the Ste20 kinase family. MST4 and MST3 are often referred to as serine/threonine-protein kinases 26 and 24 respectively while STK25 is known as Ste20-like kinase. These proteins share a common modular architecture consisting of a kinase domain and various regulatory regions. The molecular masses are approximately 50 kDa for MST4 and MST3 and around 54 kDa for STK25. You can find these proteins expressed in multiple tissues including brain testis and spleen.

Biological function summary

MST4 MST3 and STK25 regulate cell growth proliferation and apoptosis. These proteins interact with various cellular components to form signaling complexes that influence cellular responses. MST3 and MST4 can activate apoptosis signal-regulating kinase 1 (ASK1) which leads to the apoptosis pathway. STK25 plays a role in the Golgi apparatus' functionality affecting cellular trafficking and signal transduction.

Pathways

MST4 MST3 and STK25 are involved in critical signaling pathways like the Hippo pathway and MAPK signaling. Within these pathways MST4 and MST3 together associate with MOB1 and LATS1/2 enhancing their functions. STK25 interacts with STRAD and LKB1 contributing to cell polarity regulation and cellular response to external stimuli.

Associated diseases and disorders

Altered expression and mutations in MST4 MST3 and STK25 associate with cancer and neurological disorders. Cancer progression links strongly with these kinases' dysregulation due to their role in apoptosis and cell proliferation where MST4 activates CST complex involved in these diseases. Neurodegenerative disorders involve STK25 connected via its regulatory influence on cellular structures and its interactions with Huntington protein influencing disease pathophysiology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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