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AB247489

Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal STK25 phospho T174 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

SOK1, YSK1, STK25, Serine/threonine-protein kinase 25, Ste20-like kinase, Sterile 20/oxidant stress-response kinase 1, SOK-1, Ste20/oxidant stress response kinase 1, MST3, STK3, STK24, Serine/threonine-protein kinase 24, Mammalian STE20-like protein kinase 3, STE20-like kinase MST3, MST-3, MASK, MST4, STK26, Serine/threonine-protein kinase 26, MST3 and SOK1-related kinase, Mammalian STE20-like protein kinase 4, Serine/threonine-protein kinase MASK, MST-4, STE20-like kinase MST4

1 Images
Western blot - Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] - BSA and Azide free (AB247489)
  • WB

Unknown

Western blot - Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] - BSA and Azide free (AB247489)

This data was developed using ab76579, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y] (<a href='/en-us/products/primary-antibodies/mst4-mst3-stk25-phospho-t174-t178-t190-antibody-ep2123y-ab76579'>ab76579</a>) at 1/2000 dilution

Lane 1:

HeLa cell lysates, untreated at 10 µg

Lane 2:

HeLa cell lysates, treated with calyculin A at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 47-49 kDa

Observed band size: 52 kDa

false

  • Unconjugated

    Anti-MST4 + MST3 + STK25 (phospho T174 + T178 + T190) antibody [EP2123Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2123Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab247489 is the carrier-free version of ab76579.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein kinases MST4 MST3 and STK25 are part of the Ste20 kinase family. MST4 and MST3 are often referred to as serine/threonine-protein kinases 26 and 24 respectively while STK25 is known as Ste20-like kinase. These proteins share a common modular architecture consisting of a kinase domain and various regulatory regions. The molecular masses are approximately 50 kDa for MST4 and MST3 and around 54 kDa for STK25. You can find these proteins expressed in multiple tissues including brain testis and spleen.
Biological function summary

MST4 MST3 and STK25 regulate cell growth proliferation and apoptosis. These proteins interact with various cellular components to form signaling complexes that influence cellular responses. MST3 and MST4 can activate apoptosis signal-regulating kinase 1 (ASK1) which leads to the apoptosis pathway. STK25 plays a role in the Golgi apparatus' functionality affecting cellular trafficking and signal transduction.

Pathways

MST4 MST3 and STK25 are involved in critical signaling pathways like the Hippo pathway and MAPK signaling. Within these pathways MST4 and MST3 together associate with MOB1 and LATS1/2 enhancing their functions. STK25 interacts with STRAD and LKB1 contributing to cell polarity regulation and cellular response to external stimuli.

Altered expression and mutations in MST4 MST3 and STK25 associate with cancer and neurological disorders. Cancer progression links strongly with these kinases' dysregulation due to their role in apoptosis and cell proliferation where MST4 activates CST complex involved in these diseases. Neurodegenerative disorders involve STK25 connected via its regulatory influence on cellular structures and its interactions with Huntington protein influencing disease pathophysiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed : 18782753).
See full target information STK25 phospho T174

Additional targets

STK26 phospho T178,STK24 phospho T190
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:1142-1154 PubMed40229600

2025

Longitudinal single-cell multiomic atlas of high-risk neuroblastoma reveals chemotherapy-induced tumor microenvironment rewiring.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbao Yu,Rumeysa Biyik-Sit,Yasin Uzun,Chia-Hui Chen,Anusha Thadi,Jonathan H Sussman,Minxing Pang,Chi-Yun Wu,Liron D Grossmann,Peng Gao,David W Wu,Aliza Yousey,Mei Zhang,Christina S Turn,Zhan Zhang,Shovik Bandyopadhyay,Jeffrey Huang,Tasleema Patel,Changya Chen,Daniel Martinez,Lea F Surrey,Michael D Hogarty,Kathrin Bernt,Nancy R Zhang,John M Maris,Kai Tan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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