AB223692

Anti-Msx2/Hox8 antibody

Name: Anti-Msx2/Hox8 antibody (ab223692) | Abcam
Brand: Abcam Limited
SKU: AB223692
Price: 535 USD
Availability: InStock

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(11 Publications)

Rabbit Polyclonal Msx2/Hox8 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MSX2 aa 1-150.

View Alternative Names

HOX8, MSX2, Homeobox protein MSX-2, Homeobox protein Hox-8

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Msx2/Hox8 antibody (AB223692)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Msx2/Hox8 antibody (AB223692)

Formalin-fixed, paraffin-embedded human endometrium tissue stained for Msx2/Hox8 using ab223692 at 1/50 dilution in immunohistochemical analysis. Low positivity, as expected.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Msx2/Hox8 antibody (AB223692)

Formalin-fixed, paraffin-embedded human placenta tissue stained for Msx2/Hox8 using ab223692 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Msx2/Hox8 antibody (AB223692)

Formalin-fixed, paraffin-embedded human urinary bladder tissue stained for Msx2/Hox8 using ab223692 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MSX2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P35548

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Msx2 also known as Hox8 or Msh homeobox 2 is a transcription factor that binds to specific DNA sequences influencing gene expression. It belongs to the homeobox gene family and has a molecular weight of approximately 35 kDa. This protein is expressed in several tissues including the craniofacial mesenchyme limbs and skin. Msx2 plays a role in embryonic development and tissue remodeling by regulating gene transcription required for cell proliferation differentiation and apoptosis.

Biological function summary

Msx2 influences craniofacial development and limb patterning. It acts as part of a regulatory complex that coordinates processes like cranial suture closure and appendage morphogenesis. Msx2 interacts with other transcription factors and signaling molecules to modulate cellular responses during important developmental phases. Disruptions in Msx2 activity can impact normal cell function leading to developmental defects especially in the skeletal system.

Pathways

Msx2 is an important player in the Wnt and BMP signaling pathways. It interacts with proteins such as β-catenin and Smad1/Smad5 to mediate these pathways' output affecting various cell fate decisions. In the Wnt pathway Msx2 modulation helps regulate bone formation while in the BMP pathway it influences osteoblast differentiation. This integration into multiple pathways highlights Msx2’s importance in skeletal development and homeostasis.

Msx2 is associated with craniosynostosis and osteopetrosis. In craniosynostosis the premature fusion of cranial sutures is linked to dysregulated expression of Msx2. Altered interactions with proteins like Runx2 and Twist1 contribute to this pathology. In osteopetrosis Msx2's involvement in bone density regulation through its interaction with the BMP pathway is critical. Insights into Msx2’s role in these conditions can advance therapeutic development targeting these molecular interactions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Acts as a transcriptional regulator in bone development. Represses the ALPL promoter activity and antagonizes the stimulatory effect of DLX5 on ALPL expression during osteoblast differentiation. Probable morphogenetic role. May play a role in limb-pattern formation. In osteoblasts, suppresses transcription driven by the osteocalcin FGF response element (OCFRE). Binds to the homeodomain-response element of the ALPL promoter.

See full target information MSX2

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10206 PubMed39604360

2024

Single-cell transcriptome analysis reveals CD34 as a marker of human sinoatrial node pacemaker cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Amos A Lim,Delaram Pouyabahar,Mishal Ashraf,Kate Huang,Michelle Lohbihler,Brandon M Murareanu,Matthew L Chang,Maggie Kwan,Faisal J Alibhai,Thinh Tran,Amine Mazine,Michael A Laflamme,Gary D Bader,Zachary Laksman,Stephanie Protze

Nature communications 15:4985 PubMed38862515

2024

Smooth muscle NF90 deficiency ameliorates diabetic atherosclerotic calcification in male mice via FBXW7-AGER1-AGEs axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Xie,Bin Liu,Wen Qiao,Jing-Zhen He,Jie Cheng,Zhao-Yang Wang,Ya-Min Hou,Xu Zhang,Bo-Han Xu,Yun Zhang,Yu-Guo Chen,Ming-Xiang Zhang

Frontiers in cardiovascular medicine 9:1050310 PubMed36684598

2023

Role of follistatin-like 1 levels and functions in calcific aortic stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qianru Zhang,Jiawen Ye,Gan Yang,Ling Yang,Zhongli Chen,Ke Yang,Jia Teng Sun,Yan Liu

Journal of personalized medicine 11: PubMed34834487

2021

Evaluating the Expression of Candidate Homeobox Genes and Their Role in Local-Site Inflammation in Mucosal Tissue Obtained from Children with Non-Syndromic Cleft Lip and Palate.

Applications

Unspecified application

Species

Unspecified reactive species

Nityanand Jain,Mara Pilmane

American journal of translational research 13:5851-5865 PubMed34306330

2021

MSX2 inhibits the growth and migration of osteosarcoma cells by repressing SOX2.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Wu,Yi Jin,Norio Yamamoto,Akihiko Takeuchi,Shinji Miwa,Hiroyuki Tsuchiya,Zhijun Yang

JBMR plus 4:e10382 PubMed32803111

2020

Different Responsiveness of Alveolar Bone and Long Bone to Epithelial-Mesenchymal Interaction-Related Factor.

Applications

Unspecified application

Species

Unspecified reactive species

Chul Son,Moon Sil Choi,Joo-Cheol Park

Regenerative therapy 14:245-251 PubMed32455154

2020

Msx2 plays an important role in BMP6-induced osteogenic differentiation of two mesenchymal cell lines: C3H10T1/2 and C2C12.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan Cai,Jing Wang,Na Huo,Li Wen,Peng Xue,Ye Huang

FASEB journal : official publication of the Federa 33:9627-9637 PubMed31145871

2019

CTRP13 attenuates vascular calcification by regulating Runx2.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxia Li,Wenzhe Wang,Yuelin Chao,Fengxao Zhang,Cheng Wang

Nature communications 10:1203 PubMed30867423

2019

Poly(ADP-ribose) polymerase 1 accelerates vascular calcification by upregulating Runx2.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Wang,Wenjing Xu,Jie An,Minglu Liang,Yiqing Li,Fengxiao Zhang,Qiangsong Tong,Kai Huang

Journal of cellular physiology 234:17639-17648 PubMed30854680

2019

microRNA-203 promotes proliferation, differentiation, and migration of osteoblasts by upregulation of Msh homeobox 2.

Applications

Unspecified application

Species

Unspecified reactive species

Haochuan Liu,Bing Chen,Yi Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Msx2/Hox8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Msx2/Hox8 antibody (AB223692)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Msx2/Hox8 antibody (AB223692)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Msx2/Hox8 antibody (AB223692)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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