AB227720

Anti-Msx2/Hox8 antibody

Name: Anti-Msx2/Hox8 antibody (ab227720) | Abcam
Brand: Abcam Limited
SKU: AB227720
Price: 525 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal Msx2/Hox8 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MSX2.

View Alternative Names

HOX8, MSX2, Homeobox protein MSX-2, Homeobox protein Hox-8

3 Images

Supplier Data

Western blot - Anti-Msx2/Hox8 antibody (AB227720)

12% SDS-PAGE

All lanes:

Western blot - Anti-Msx2/Hox8 antibody (ab227720) at 1/500 dilution

All lanes:

HL-60 (human promyelocytic leukemia cell line) whole cell lysate at 30 µg

Predicted band size: 29 kDa

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Supplier Data

Western blot - Anti-Msx2/Hox8 antibody (AB227720)

12% SDS-PAGE

All lanes:

Western blot - Anti-Msx2/Hox8 antibody (ab227720) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 29 kDa

true

Supplier Data

Western blot - Anti-Msx2/Hox8 antibody (AB227720)

12% SDS-PAGE

All lanes:

Western blot - Anti-Msx2/Hox8 antibody (ab227720) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg

Lane 2:

C8D30 whole cell lysate at 30 µg

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg

Lane 4:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 30 µg

Lane 5:

C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg

Predicted band size: 29 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

applications

Immunogen

Recombinant Fragment Protein within Human MSX2. The exact immunogen used to generate this antibody is proprietary information.

P35548

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Msx2 also known as Hox8 or Msh homeobox 2 is a transcription factor that binds to specific DNA sequences influencing gene expression. It belongs to the homeobox gene family and has a molecular weight of approximately 35 kDa. This protein is expressed in several tissues including the craniofacial mesenchyme limbs and skin. Msx2 plays a role in embryonic development and tissue remodeling by regulating gene transcription required for cell proliferation differentiation and apoptosis.

Biological function summary

Msx2 influences craniofacial development and limb patterning. It acts as part of a regulatory complex that coordinates processes like cranial suture closure and appendage morphogenesis. Msx2 interacts with other transcription factors and signaling molecules to modulate cellular responses during important developmental phases. Disruptions in Msx2 activity can impact normal cell function leading to developmental defects especially in the skeletal system.

Pathways

Msx2 is an important player in the Wnt and BMP signaling pathways. It interacts with proteins such as β-catenin and Smad1/Smad5 to mediate these pathways' output affecting various cell fate decisions. In the Wnt pathway Msx2 modulation helps regulate bone formation while in the BMP pathway it influences osteoblast differentiation. This integration into multiple pathways highlights Msx2’s importance in skeletal development and homeostasis.

Msx2 is associated with craniosynostosis and osteopetrosis. In craniosynostosis the premature fusion of cranial sutures is linked to dysregulated expression of Msx2. Altered interactions with proteins like Runx2 and Twist1 contribute to this pathology. In osteopetrosis Msx2's involvement in bone density regulation through its interaction with the BMP pathway is critical. Insights into Msx2’s role in these conditions can advance therapeutic development targeting these molecular interactions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional regulator in bone development. Represses the ALPL promoter activity and antagonizes the stimulatory effect of DLX5 on ALPL expression during osteoblast differentiation. Probable morphogenetic role. May play a role in limb-pattern formation. In osteoblasts, suppresses transcription driven by the osteocalcin FGF response element (OCFRE). Binds to the homeodomain-response element of the ALPL promoter.

See full target information MSX2

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7228 PubMed40764291

2025

Targeting Msx2 as a brake in the fusion fate of osteoclasts and an anabolic therapy in pre-clinical models of osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qingliang Ma,Shiyu Wang,Hong Xue,Linhui Ni,Putao Yuan,Yang Shen,Bingjie Zheng,Qingqing Wang,Jiateng Zhang,Haoming Wang,Hongwei Xie,Chao Jiang,An Qin,Shunwu Fan,Ziang Xie,Zhiwei Jie

PloS one 20:e0323058 PubMed40354352

2025

The lncRNA SENCR knockdown alleviates vascular calcification via miR-4731-5p by suppressing endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yongpan Huang,Wei Zhan,Chong Song,Meihua Tan,Li Wu,Sina Wu

Nature communications 15:4985 PubMed38862515

2024

Smooth muscle NF90 deficiency ameliorates diabetic atherosclerotic calcification in male mice via FBXW7-AGER1-AGEs axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Xie,Bin Liu,Wen Qiao,Jing-Zhen He,Jie Cheng,Zhao-Yang Wang,Ya-Min Hou,Xu Zhang,Bo-Han Xu,Yun Zhang,Yu-Guo Chen,Ming-Xiang Zhang

Molecular carcinogenesis 63:1449-1466 PubMed38801356

2024

Curcuminoid PBPD induces cuproptosis and endoplasmic reticulum stress in cervical cancer via the Notch1/RBP-J/NRF2/FDX1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Jie Zhang,Mengna Shi,Yang Yu,Rongying Ou,Ren-Shan Ge,Ping Duan

Cell reports 43:114134 PubMed38662542

2024

The MYCN 5' UTR as a therapeutic target in neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Marina P Volegova,Lauren E Brown,Ushashi Banerjee,Ruben Dries,Bandana Sharma,Alyssa Kennedy,John A Porco,Rani E George

Journal of cardiovascular pharmacology 78:411-421 PubMed34132687

2021

Ginkgo Biloba Extract EGB761 Alleviates Warfarin-induced Aortic Valve Calcification Through the BMP2/Smad1/5/Runx2 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Liu,Cuiying Liu,Chunqi Qian,George Abela,Wei Sun,Xiangqing Kong

Stem cell research & therapy 12:235 PubMed33849640

2021

Bone marrow mesenchymal stem cell exosomes suppress phosphate-induced aortic calcification via SIRT6-HMGB1 deacetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqian Wei,Xiaodong Guo,Lijie Gu,Jieshuang Jia,Man Yang,Weijie Yuan,Shu Rong

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Msx2/Hox8 antibody

Western blot - Anti-Msx2/Hox8 antibody (AB227720)

Western blot - Anti-Msx2/Hox8 antibody (AB227720)

Western blot - Anti-Msx2/Hox8 antibody (AB227720)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise