AB249135

Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal MSY2/YBOX2/YBX2 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

CSDA3, MSY2, YBX2, Y-box-binding protein 2, Contrin, DNA-binding protein C, Germ cell-specific Y-box-binding protein, MSY2 homolog, Dbpc

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] - BSA and Azide free (AB249135)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] - BSA and Azide free (AB249135)

This data was developed using ab154829, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HepG2 cells labeling MSY2/YBOX2/YBX2 with ab154829 at 1/50 dilution.

Unknown

Western blot - Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] - BSA and Azide free (AB249135)

This data was developed using ab154829, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] (<a href='/en-us/products/primary-antibodies/msy2-ybox2-ybx2-antibody-epr28142-ab154829'>ab154829</a>) at 1/1000 dilution

Lane 1:

Human seminoma lysate at 10 µg

Lane 2:

Mouse testis lysate at 10 µg

Lane 3:

Rat testis lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2814(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab249135 is the carrier-free version of ab154829.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MSY2 also known as YBOX2 or YBX2 is a protein involved in gene regulation specifically affecting mRNA stability and translation. The protein has a molecular mass of approximately 36 kDa. MSY2 is a germ cell-specific protein primarily expressed in testes and ovaries. It plays an important role during gametogenesis and early embryogenesis by binding with mRNA and forming messenger ribonucleoprotein (mRNP) complexes and therefore controlling mRNA metabolism.

Biological function summary

MSY2 functions as a regulator of mRNA translation and storage. It is part of a ribonucleoprotein complex that safeguards mRNAs during gametogenesis. This protein displays specificity in binding to Y-box motifs on mRNA which allows it to regulate gene expression and protein synthesis at a posttranscriptional level. Its function ensures correct development of germ cells contributing to reproductive success.

Pathways

MSY2 integrates into posttranscriptional regulatory mechanisms and is important for mRNA stability within these pathways. One such pathway involves cytoplasmic deadenylation and translational control during oocyte maturation. MSY2’s role also extends to working closely with other proteins such as YBX1 which shares similar functions in mRNA regulation further highlighting the interconnected nature of its activity.

MSY2’s deregulation can lead to fertility issues particularly affecting spermatogenesis. Aberrant expression of MSY2 affects mRNA stability and may result in testicular dysfunction. It also connects with proteins like DAZL which is another germ cell-specific regulator suggesting that perturbations in this network can affect germ cell viability and lead to reproductive disorders. Proper expression and regulation of MSY2 are therefore essential for maintaining reproductive health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Major constituent of messenger ribonucleoprotein particles (mRNPs). Involved in the regulation of the stability and/or translation of germ cell mRNAs. Binds to Y-box consensus promoter element. Binds to full-length mRNA with high affinity in a sequence-independent manner. Binds to short RNA sequences containing the consensus site 5'-UCCAUCA-3' with low affinity and limited sequence specificity. Its binding with maternal mRNAs is necessary for its cytoplasmic retention. May mark specific mRNAs (those transcribed from Y-box promoters) in the nucleus for cytoplasmic storage, thereby linking transcription and mRNA storage/translational delay (By similarity).

See full target information YBX2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 60:2744-2760.e9 PubMed40460835

2025

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano De Tito,Eugenia Almacellas,Daniel Dai Yu,Emily Millard,Wenxin Zhang,Cecilia de Heus,Christophe Queval,Javier H Hervás,Enrica Pellegrino,Ioanna Panagi,Ditte Fogde,Teresa L M Thurston,Judith Klumperman,Maximiliano Gutierrez,Sharon A Tooze

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] - BSA and Azide free (AB249135)

Western blot - Anti-MSY2/YBOX2/YBX2 antibody [EPR2814(2)] - BSA and Azide free (AB249135)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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