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Rabbit Recombinant Monoclonal MT-ND1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 30 publications.


Images

Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (AB181848), expandable thumbnail
  • Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (AB181848), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/20000

Notes

This antibody is unsuitable for detecting cell line samples.

Species

Rat

Dilution info

1/1000 - 1/20000

Notes

This antibody is unsuitable for detecting cell line samples.

Species

Human

Dilution info

1/1000 - 1/20000

Notes

This antibody is unsuitable for detecting cell line samples.

Associated Products

Select an associated product type

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Target data

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:1959619). Essential for the catalytic activity and assembly of complex I (PubMed:1959619, PubMed:26929434).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal MT-ND1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 30 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR13466(2)

Purification technique

Affinity purification Protein A

Specificity

This antibody is unsuitable for detecting cell line samples.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MT-ND1 also known as mitochondrial ND1 or NADH dehydrogenase 1 is a subunit of the enzyme NADH: ubiquinone oxidoreductase commonly called Complex I. This protein has a mass of approximately 35 kDa. It locates in the inner mitochondrial membrane where it serves a role in electron transport. MT-ND1 helps catalyze the transfer of electrons from NADH to ubiquinone an important step in the mitochondrial respiratory chain.

Biological function summary

The function of MT-ND1 is vital for ATP production. It forms part of the Complex I assembly within the mitochondrial membrane. Complex I is the first enzyme in the electron transport chain and initiates the transfer of electrons which ultimately produces the proton gradient needed by ATP synthase to generate adenosine triphosphate (ATP). This energy conversion process is essential for cellular energy homeostasis.

Pathways

MT-ND1 is integral to the oxidative phosphorylation pathway which is an important process for energy production in cells. Through the electron transport chain MT-ND1 is linked to other mitochondrial proteins such as MT-ND2 and MT-ND3 as they form subunits of the same complex. These subunits collectively contribute to maintaining the efficiency and regulation of cellular respiration.

Associated diseases and disorders

Defects in MT-ND1 are associated with mitochondrial diseases such as Leber's hereditary optic neuropathy (LHON) and mitochondrial encephalomyopathy. MT-ND1 mutations may disrupt the electron transport chain leading to decreased ATP production and increased reactive oxygen species. These conditions are also related to other components of Complex I including MT-ND4 and MT-ND6 further affecting the functioning of the mitochondrial respiratory machinery.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (ab181848), expandable thumbnail

    Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (ab181848)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (ab181848) at 1/5000 dilution

    Lane 1: Human heart lysates at 15 µg

    Lane 2: Mouse heart lysates at 15 µg

    Lane 3: Rat heart lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa

    Blocking and diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181848).

  • Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (ab181848), expandable thumbnail

    Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (ab181848)

    All lanes: Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (ab181848) at 1/20000 dilution

    Lane 1: Human fetal heart lysate at 20 µg

    Lane 2: Human fetal muscle lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution

    Predicted band size: 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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