AB181848

Anti-MT-ND1 antibody [EPR13466(2)]

Name: Anti-MT-ND1 antibody [EPR13466(2)] Rabbit monoclonal (ab181848) | Abcam
Brand: Abcam Limited
SKU: AB181848
Price: 590 USD
Availability: InStock
Rating: 4 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(44 Publications)

Rabbit Recombinant Monoclonal MT-ND1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 44 publications.

View Alternative Names

MTND1, NADH1, ND1, MT-ND1, NADH-ubiquinone oxidoreductase chain 1, NADH dehydrogenase subunit 1

2 Images

Lab

Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (AB181848)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (ab181848) at 1/5000 dilution

Lane 1:

Human heart lysates at 15 µg

Lane 2:

Mouse heart lysates at 15 µg

Lane 3:

Rat heart lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 36 kDa

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Supplier Data

Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (AB181848)

All lanes:

Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (ab181848) at 1/20000 dilution

Lane 1:

Human fetal heart lysate at 20 µg

Lane 2:

Human fetal muscle lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 36 kDa

false

HRP

HRP Anti-MT-ND1 antibody [EPR13466(2)]
Carrier free

Anti-MT-ND1 antibody [EPR13466(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13466(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is unsuitable for detecting cell line samples.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "This antibody is unsuitable for detecting cell line samples." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "This antibody is unsuitable for detecting cell line samples." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "This antibody is unsuitable for detecting cell line samples." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MT-ND1 also known as mitochondrial ND1 or NADH dehydrogenase 1 is a subunit of the enzyme NADH: ubiquinone oxidoreductase commonly called Complex I. This protein has a mass of approximately 35 kDa. It locates in the inner mitochondrial membrane where it serves a role in electron transport. MT-ND1 helps catalyze the transfer of electrons from NADH to ubiquinone an important step in the mitochondrial respiratory chain.

Biological function summary

The function of MT-ND1 is vital for ATP production. It forms part of the Complex I assembly within the mitochondrial membrane. Complex I is the first enzyme in the electron transport chain and initiates the transfer of electrons which ultimately produces the proton gradient needed by ATP synthase to generate adenosine triphosphate (ATP). This energy conversion process is essential for cellular energy homeostasis.

Pathways

MT-ND1 is integral to the oxidative phosphorylation pathway which is an important process for energy production in cells. Through the electron transport chain MT-ND1 is linked to other mitochondrial proteins such as MT-ND2 and MT-ND3 as they form subunits of the same complex. These subunits collectively contribute to maintaining the efficiency and regulation of cellular respiration.

Defects in MT-ND1 are associated with mitochondrial diseases such as Leber's hereditary optic neuropathy (LHON) and mitochondrial encephalomyopathy. MT-ND1 mutations may disrupt the electron transport chain leading to decreased ATP production and increased reactive oxygen species. These conditions are also related to other components of Complex I including MT-ND4 and MT-ND6 further affecting the functioning of the mitochondrial respiratory machinery.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 1959619). Essential for the catalytic activity and assembly of complex I (PubMed : 1959619, PubMed : 26929434).

See full target information MT-ND1

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Publications (44)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 31: PubMed40183404

2025

α‑ketoglutarate protects against septic cardiomyopathy by improving mitochondrial mitophagy and fission.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wu,Qiong Ma,Bo-Tao Li,Shuang Shi,Gong-Chang Guan,Jun-Kui Wang,Bao-Yao Xue,Zhong-Wei Liu

Molecular medicine (Cambridge, Mass.) 31:93 PubMed40075333

2025

WBP1 regulates mitochondrial function and ferroptosis to modulate chemoresistance in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Dachuan Qi,Guijie Ge,Ning Cao,Xiangdong Liu,Na Zhu,Feng Li,Xiang Huang,Kui Yu,Jinzhou Zheng,Daoheng Wang,Wenyan Yao,Lili Chen,Ziyang Dong

American journal of physiology. Cell physiology 328:C168-C178 PubMed39611618

2024

C stable isotope tracing reveals distinct fatty acid oxidation pathways in proliferative versus oxidative cells.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Ritterhoff,Timothy McMillen,Hanna Foundas,Roland Palkovacs,Gernot Poschet,Arianne Caudal,Yaxin Liu,Patrick Most,Matthew Walker,Rong Tian

Cell reports. Medicine 5:101753 PubMed39357525

2024

Leucine restriction ameliorates Fusobacterium nucleatum-driven malignant progression and radioresistance in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Songhe Guo,Shan Xing,ZhenYu Wu,Fangfang Chen,Xiaoyun Pan,Qifan Li,Wanli Liu,Ge Zhang

PLoS pathogens 20:e1012079 PubMed38466743

2024

Hepatitis B virus e antigen induces atypical metabolism and differentially regulates programmed cell deaths of macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Yumei Li,Christine Wu,Jiyoung Lee,Qiqi Ning,Juhyeon Lim,Hyungjin Eoh,Sean Wang,Benjamin P Hurrell,Omid Akbari,Jing-Hsiung James Ou

Circulation 148:2038-2057 PubMed37965787

2023

Raising NAD Level Stimulates Short-Chain Dehydrogenase/Reductase Proteins to Alleviate Heart Failure Independent of Mitochondrial Protein Deacetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew A Walker,Hongye Chen,Aprajita Yadav,Julia Ritterhoff,Outi Villet,Tim McMillen,Yuliang Wang,Hayley Purcell,Danijel Djukovic,Daniel Raftery,Nina Isoherranen,Rong Tian

Nature cell biology 25:1575-1589 PubMed37770567

2023

Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas J Kramer,Gyan Prakash,R Stefan Isaac,Karine Choquet,Iliana Soto,Boryana Petrova,Hope E Merens,Naama Kanarek,L Stirling Churchman

Cardiovascular research 119:2213-2229 PubMed37395010

2023

Tissue-specific differences in the assembly of mitochondrial Complex I are revealed by a novel ENU mutation in ECSIT.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Nicol,Sara Falcone,Andrew Blease,Pratik Vikhe,Gabriele Civiletto,Saleh Salman Omairi,Carlo Viscomi,Ketan Patel,Paul K Potter

Life science alliance 6: PubMed37321846

2023

Time-resolved proteomic analyses of senescence highlight metabolic rewiring of mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Yong Kim,Ilian Atanassov,Frederik Dethloff,Lara Kroczek,Thomas Langer

Journal of cardiovascular pharmacology 81:259-269 PubMed36668724

2023

Evaluation of Coenzyme Q10 (CoQ10) Deficiency and Therapy in Mouse Models of Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Tian Pu,Wei Wu,Pei-Da Liang,Jin-Chan Du,Sheng-Li Han,Xiu-Ling Deng,Xiao-Jun Du

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MT-ND1 antibody [EPR13466(2)]

Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (AB181848)

Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (AB181848)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (44)

Product promise