Rabbit Recombinant Monoclonal MT-ND1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 30 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA
Liquid
Monoclonal
WB | |
---|---|
Human | Tested |
Mouse | Tested |
Rat | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/20000 | Notes This antibody is unsuitable for detecting cell line samples. |
Species Rat | Dilution info 1/1000 - 1/20000 | Notes This antibody is unsuitable for detecting cell line samples. |
Species Human | Dilution info 1/1000 - 1/20000 | Notes This antibody is unsuitable for detecting cell line samples. |
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Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:1959619). Essential for the catalytic activity and assembly of complex I (PubMed:1959619, PubMed:26929434).
MTND1, NADH1, ND1, NADH1, ND1, MTND1, MT-ND1, NADH-ubiquinone oxidoreductase chain 1, NADH dehydrogenase subunit 1
Rabbit Recombinant Monoclonal MT-ND1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 30 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA
Liquid
Monoclonal
EPR13466(2)
Affinity purification Protein A
This antibody is unsuitable for detecting cell line samples.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
MT-ND1 also known as mitochondrial ND1 or NADH dehydrogenase 1 is a subunit of the enzyme NADH: ubiquinone oxidoreductase commonly called Complex I. This protein has a mass of approximately 35 kDa. It locates in the inner mitochondrial membrane where it serves a role in electron transport. MT-ND1 helps catalyze the transfer of electrons from NADH to ubiquinone an important step in the mitochondrial respiratory chain.
The function of MT-ND1 is vital for ATP production. It forms part of the Complex I assembly within the mitochondrial membrane. Complex I is the first enzyme in the electron transport chain and initiates the transfer of electrons which ultimately produces the proton gradient needed by ATP synthase to generate adenosine triphosphate (ATP). This energy conversion process is essential for cellular energy homeostasis.
MT-ND1 is integral to the oxidative phosphorylation pathway which is an important process for energy production in cells. Through the electron transport chain MT-ND1 is linked to other mitochondrial proteins such as MT-ND2 and MT-ND3 as they form subunits of the same complex. These subunits collectively contribute to maintaining the efficiency and regulation of cellular respiration.
Defects in MT-ND1 are associated with mitochondrial diseases such as Leber's hereditary optic neuropathy (LHON) and mitochondrial encephalomyopathy. MT-ND1 mutations may disrupt the electron transport chain leading to decreased ATP production and increased reactive oxygen species. These conditions are also related to other components of Complex I including MT-ND4 and MT-ND6 further affecting the functioning of the mitochondrial respiratory machinery.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (ab181848) at 1/5000 dilution
Lane 1: Human heart lysates at 15 µg
Lane 2: Mouse heart lysates at 15 µg
Lane 3: Rat heart lysates at 15 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Blocking and diluting buffer: 5% NFDM/TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181848).
All lanes: Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (ab181848) at 1/20000 dilution
Lane 1: Human fetal heart lysate at 20 µg
Lane 2: Human fetal muscle lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution
Predicted band size: 36 kDa
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