JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB232594

Anti-MT-ND1 antibody [EPR13466(2)] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal MT-ND1 antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

MTND1, NADH1, ND1, MT-ND1, NADH-ubiquinone oxidoreductase chain 1, NADH dehydrogenase subunit 1

1 Images
Western blot - Anti-MT-ND1 antibody [EPR13466(2)] - BSA and Azide free (AB232594)
  • WB

Lab

Western blot - Anti-MT-ND1 antibody [EPR13466(2)] - BSA and Azide free (AB232594)

Blocking and diluting buffer : 5% NFDM/TBST

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181848).

All lanes:

Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (<a href='/en-us/products/primary-antibodies/mt-nd1-antibody-epr134662-ab181848'>ab181848</a>) at 1/5000 dilution

Lane 1:

Human heart lysates at 15 µg

Lane 2:

Mouse heart lysates at 15 µg

Lane 3:

Rat heart lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 36 kDa

false

  • Unconjugated

    Anti-MT-ND1 antibody [EPR13466(2)]

  • HRP

    HRP Anti-MT-ND1 antibody [EPR13466(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13466(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is unsuitable for detecting cell line samples.

style
left-column

Complementary Products

Primary Antibodies

AB14705

Anti-MTCO1 antibody [1D6E1A8]

BOND RX™ Validated|Recombinant

Western blot - Anti-MTCO1 antibody [1D6E1A8] (AB14705)

  • 5
  • 44
Primary Antibodies

AB110258

Anti-MTCO2 antibody [12C4F12]

Western blot - Anti-MTCO2 antibody [12C4F12] (AB110258)

  • 5
  • 18
Primary Antibodies

AB192306

Anti-MT-ND3 antibody

Western blot - Anti-MT-ND3 antibody (AB192306)

  • 4
  • 1
Primary Antibodies

AB109025

Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (AB109025)

  • 4
  • 3
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>This antibody is unsuitable for detecting cell line samples.</p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>This antibody is unsuitable for detecting cell line samples.</p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>This antibody is unsuitable for detecting cell line samples.</p>" } } }
style
left-column

Product details

ab232594 is the carrier-free version of ab181848.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MT-ND1 also known as mitochondrial ND1 or NADH dehydrogenase 1 is a subunit of the enzyme NADH: ubiquinone oxidoreductase commonly called Complex I. This protein has a mass of approximately 35 kDa. It locates in the inner mitochondrial membrane where it serves a role in electron transport. MT-ND1 helps catalyze the transfer of electrons from NADH to ubiquinone an important step in the mitochondrial respiratory chain.
Biological function summary

The function of MT-ND1 is vital for ATP production. It forms part of the Complex I assembly within the mitochondrial membrane. Complex I is the first enzyme in the electron transport chain and initiates the transfer of electrons which ultimately produces the proton gradient needed by ATP synthase to generate adenosine triphosphate (ATP). This energy conversion process is essential for cellular energy homeostasis.

Pathways

MT-ND1 is integral to the oxidative phosphorylation pathway which is an important process for energy production in cells. Through the electron transport chain MT-ND1 is linked to other mitochondrial proteins such as MT-ND2 and MT-ND3 as they form subunits of the same complex. These subunits collectively contribute to maintaining the efficiency and regulation of cellular respiration.

Defects in MT-ND1 are associated with mitochondrial diseases such as Leber's hereditary optic neuropathy (LHON) and mitochondrial encephalomyopathy. MT-ND1 mutations may disrupt the electron transport chain leading to decreased ATP production and increased reactive oxygen species. These conditions are also related to other components of Complex I including MT-ND4 and MT-ND6 further affecting the functioning of the mitochondrial respiratory machinery.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 1959619). Essential for the catalytic activity and assembly of complex I (PubMed : 1959619, PubMed : 26929434).
See full target information MT-ND1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 7:493-507 PubMed39972217

2025

Pro-inflammatory macrophages produce mitochondria-derived superoxide by reverse electron transport at complex I that regulates IL-1β release during NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Alva M Casey,Dylan G Ryan,Hiran A Prag,Suvagata Roy Chowdhury,Eloïse Marques,Keira Turner,Anja V Gruszczyk,Ming Yang,Dane M Wolf,Jan Lj Miljkovic,Joyce Valadares,Patrick F Chinnery,Richard C Hartley,Christian Frezza,Julien Prudent,Michael P Murphy
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com