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Rabbit Recombinant Monoclonal MT-ND1 antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples.

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Images

Western blot - Anti-MT-ND1 antibody [EPR13466(2)] - BSA and Azide free (AB232594), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Expected
Rat
Expected

Tested
Tested

Species

Human

Dilution info

-

Notes

This antibody is unsuitable for detecting cell line samples.

Expected
Expected

Species

Rat

Dilution info

-

Notes

This antibody is unsuitable for detecting cell line samples.

Species

Mouse

Dilution info

-

Notes

This antibody is unsuitable for detecting cell line samples.

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed:1959619). Essential for the catalytic activity and assembly of complex I (PubMed:1959619, PubMed:26929434).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal MT-ND1 antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR13466(2)

Purification technique

Affinity purification Protein A

Specificity

This antibody is unsuitable for detecting cell line samples.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab232594 is the carrier-free version of Anti-MT-ND1 antibody [EPR13466(2)] ab181848.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MT-ND1 also known as mitochondrial ND1 or NADH dehydrogenase 1 is a subunit of the enzyme NADH: ubiquinone oxidoreductase commonly called Complex I. This protein has a mass of approximately 35 kDa. It locates in the inner mitochondrial membrane where it serves a role in electron transport. MT-ND1 helps catalyze the transfer of electrons from NADH to ubiquinone an important step in the mitochondrial respiratory chain.

Biological function summary

The function of MT-ND1 is vital for ATP production. It forms part of the Complex I assembly within the mitochondrial membrane. Complex I is the first enzyme in the electron transport chain and initiates the transfer of electrons which ultimately produces the proton gradient needed by ATP synthase to generate adenosine triphosphate (ATP). This energy conversion process is essential for cellular energy homeostasis.

Pathways

MT-ND1 is integral to the oxidative phosphorylation pathway which is an important process for energy production in cells. Through the electron transport chain MT-ND1 is linked to other mitochondrial proteins such as MT-ND2 and MT-ND3 as they form subunits of the same complex. These subunits collectively contribute to maintaining the efficiency and regulation of cellular respiration.

Associated diseases and disorders

Defects in MT-ND1 are associated with mitochondrial diseases such as Leber's hereditary optic neuropathy (LHON) and mitochondrial encephalomyopathy. MT-ND1 mutations may disrupt the electron transport chain leading to decreased ATP production and increased reactive oxygen species. These conditions are also related to other components of Complex I including MT-ND4 and MT-ND6 further affecting the functioning of the mitochondrial respiratory machinery.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-MT-ND1 antibody [EPR13466(2)] - BSA and Azide free (ab232594), expandable thumbnail

    Western blot - Anti-MT-ND1 antibody [EPR13466(2)] - BSA and Azide free (ab232594)

    Blocking and diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MT-ND1 antibody [EPR13466(2)] ab181848).

    All lanes: Western blot - Anti-MT-ND1 antibody [EPR13466(2)] (Anti-MT-ND1 antibody [EPR13466(2)] ab181848) at 1/5000 dilution

    Lane 1: Human heart lysates at 15 µg

    Lane 2: Mouse heart lysates at 15 µg

    Lane 3: Rat heart lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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