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AB204977

Anti-MT-ND3 antibody

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(1 Publication)

Rabbit Polyclonal MT-ND3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MT-ND3 aa 1-100.

View Alternative Names

MTND3, NADH3, ND3, MT-ND3, NADH-ubiquinone oxidoreductase chain 3, NADH dehydrogenase subunit 3

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MT-ND3 antibody (AB204977)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MT-ND3 antibody (AB204977)

Immunofluorescence analysis of MCF7 cells (Fixation/Permeabilization : PFA/Triton X-100) labeling MT-ND3 using ab204977 at 4 μg/mL.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND3 antibody (AB204977)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND3 antibody (AB204977)

Immunohistochemical analysis of paraffin-embedded human rectum tissue labeling MT-ND3 using ab204977 at 1/20 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MT-ND3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P03897

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MT-ND3 also known as NADH-ubiquinone oxidoreductase chain 3 is a subunit of Complex I of the mitochondrial respiratory chain. It operates as part of the first enzyme in the electron transport chain which is important for ATP production. With an approximate molecular mass of 12 kDa MT-ND3 is expressed in the inner mitochondrial membrane. This subunit plays a critical role in the transfer of electrons from NADH to ubiquinone facilitating energy conversion within the cell.
Biological function summary

MT-ND3 contributes to essential energy metabolism processes by being integrated into Complex I also known as NADH: ubiquinone oxidoreductase. This complex is integral to maintaining cellular energy homeostasis as it catalyzes the initial step in oxidative phosphorylation. As part of the large multi-subunit Complex I MT-ND3 along with other subunits assists in proton translocation across the mitochondrial membrane which is indispensable for generating an electrochemical gradient used in ATP synthesis.

Pathways

The MT-ND3 subunit plays a role in the electron transport chain and oxidative phosphorylation pathways. It collaborates with other complex subunits like MT-ND1 and MT-ND2 to facilitate electron flow and proton pumping. These pathways are central to cellular respiration influencing how cells extract energy from nutrients. MT-ND3 is indirectly involved with other proteins like cytochrome c which further participates in the cascade of electron transfer leading to ATP production.

Mutations in the MT-ND3 gene have been linked to mitochondrial disorders such as Leber's hereditary optic neuropathy (LHON) and mitochondrial encephalomyopathy. These conditions often manifest due to disrupted oxidative phosphorylation leading to reduced ATP availability and increased reactive oxygen species production. MT-ND3 dysfunction might also impact other mitochondrial components like MT-CO1 as these proteins collectively impact energy production and cellular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 25118196). Essential for the catalytic activity of complex I (PubMed : 25118196).
See full target information MT-ND3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical epigenetics 12:173 PubMed33203470

2020

The tumor suppressor Zinc finger protein 471 suppresses breast cancer growth and metastasis through inhibiting AKT and Wnt/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chunfang Tao,Juan Luo,Jun Tang,Danfeng Zhou,Shujun Feng,Zhu Qiu,Thomas C Putti,Tingxiu Xiang,Qiao Tao,Lili Li,Guosheng Ren
View all publications
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Product promise

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