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AB138136

Anti-MT-ND5 antibody

0

(1 Review)

|

(3 Publications)

Rabbit Polyclonal MT-ND5 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MT-ND5.

View Alternative Names

MTND5, NADH5, ND5, MT-ND5, NADH-ubiquinone oxidoreductase chain 5, NADH dehydrogenase subunit 5

1 Images
Western blot - Anti-MT-ND5 antibody (AB138136)
  • WB

Unknown

Western blot - Anti-MT-ND5 antibody (AB138136)

All lanes:

Western blot - Anti-MT-ND5 antibody (ab138136) at 1/500 dilution

All lanes:

Jurkat cell lysate at 30 µg

Predicted band size: 67 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human MT-ND5. The exact immunogen used to generate this antibody is proprietary information.

P03915

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab138136 was affinity purified from rabbit antiserum by immunogenic peptide affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MT-ND5 also known as NADH-ubiquinone oxidoreductase chain 5 is a subunit of mitochondrial complex I found in the inner mitochondrial membrane. This protein weighing approximately 74 kDa plays a central role in the electron transport chain facilitating the transfer of electrons from NADH to ubiquinone. Expression of MT-ND5 occurs ubiquitously in tissues with high energy demands such as the heart brain and skeletal muscle where efficient ATP production is essential.
Biological function summary

MT-ND5 functions as an integral component of mitochondrial complex I which is the first enzyme complex in the electron transport chain. By participating in this complex MT-ND5 contributes to the generation of a proton gradient across the inner mitochondrial membrane driving ATP synthesis through oxidative phosphorylation. Malfunction of MT-ND5 impairs energy production which can lead to a cascade of cellular energy deficiencies given its role within the complex.

Pathways

MT-ND5 associates with the oxidative phosphorylation pathway. It is important for maintaining aerobic energy production by linking NADH oxidation with ubiquinone reduction. Other proteins like MT-ND1 and MT-ND4L also participate in electron transport chain pathways working alongside MT-ND5 to ensure effective electron transfer and mitochondrial respiration. Its function within these pathways highlights its importance in metabolic regulation.

MT-ND5 mutations have associations with mitochondrial disorders such as Leigh syndrome and mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS). Defective MT-ND5 affects the electron transport chain leading to energy metabolism disruption in affected tissues. These disorders often involve other mitochondrial proteins such as MT-ND1 where combined mutations further exacerbate the dysfunction of mitochondrial capacity and highlight the profound impact on human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 15250827). Essential for the catalytic activity and assembly of complex I (PubMed : 15250827).
See full target information MT-ND5
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 27:1240-1255 PubMed40760247

2025

A programmed decline in ribosome levels governs human early neurodevelopment.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyang Ni,Yudong Wei,Barbara Vona,Dayea Park,Yulei Wei,Daniel A Schmitz,Yi Ding,Masahiro Sakurai,Emily Ballard,Leijie Li,Yan Liu,Ashwani Kumar,Chao Xing,Shenlu Qin,Sangin Kim,Martina Foglizzo,Jianchao Zhao,Hyung-Goo Kim,Cumhur Ekmekci,Ehsan Ghayoor Karimiani,Shima Imannezhad,Fatemeh Eghbal,Reza Shervin Badv,Eva Maria Christina Schwaibold,Mohammadreza Dehghani,Mohammad Yahya Vahidi Mehrjardi,Zahra Metanat,Hosein Eslamiyeh,Ebtissal Khouj,Saleh Mohammed Nasser Alhajj,Aziza Chedrawi,Khushnooda Ramzan,Jamil A Hashmi,Majed M Alluqmani,Sulman Basit,Danai Veltra,Nikolaos M Marinakis,Georgios Niotakis,Pelagia Vorgia,Christalena Sofocleous,Hane Lee,Won Chan Jeong,Muhammad Umair,Muhammad Bilal,César Augusto Pinheiro Ferreira Alves,Matthew Sieber,Michael Kruer,Henry Houlden,Fowzan S Alkuraya,Elton Zeqiraj,Roger A Greenberg,Can Cenik,Leqian Yu,Reza Maroofian,Jun Wu,Michael Buszczak

Scientific reports 15:10925 PubMed40157968

2025

Characterization of a new mutation of mitochondrial ND6 gene in hepatocellular carcinoma and its effects on respiratory complex I.

Applications

Unspecified application

Species

Unspecified reactive species

Veronica Bazzani,Deepali L Kundnani,Mara Equisoain Redin,Francesca Agostini,Kirti Chhatlani,Angelo Corso Faini,Jakub Poziemski,Umberto Baccarani,Pawel Siedlecki,Silvia Deaglio,Francesca Storici,Carlo Vascotto

The Journal of biological chemistry 295:13224-13238 PubMed32723871

2020

Complex I mutations synergize to worsen the phenotypic expression of Leber's hereditary optic neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yanchun Ji,Juanjuan Zhang,Yuanyuan Lu,Qiuzi Yi,Mengquan Chen,Shipeng Xie,Xiaoting Mao,Yun Xiao,Feilong Meng,Minglian Zhang,Rulai Yang,Min-Xin Guan
View all publications
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Product promise

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