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AB230509

Anti-MT-ND5 antibody

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(3 Publications)

Rabbit Polyclonal MT-ND5 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MT-ND5 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

MTND5, NADH5, ND5, MT-ND5, NADH-ubiquinone oxidoreductase chain 5, NADH dehydrogenase subunit 5

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND5 antibody (AB230509)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND5 antibody (AB230509)

Formalin-fixed, paraffin-embedded human skeletal tissue stained for MT-ND5 using ab230509 at 1/50 dilution in immunhistochemical analysis.

Flow Cytometry (Intracellular) - Anti-MT-ND5 antibody (AB230509)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MT-ND5 antibody (AB230509)

Intracellular flow cytometric analysis of CRRF-CEM (human T cell lymphoblast cell line) labelling MT-ND5 using ab230509 at 1/10 dilution (bottom histogram) compared to the negetive control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot - Anti-MT-ND5 antibody (AB230509)
  • WB

Supplier Data

Western blot - Anti-MT-ND5 antibody (AB230509)

All lanes:

Western blot - Anti-MT-ND5 antibody (ab230509) at 1/1000 dilution

All lanes:

CRRF-CEM (Human T cell lymphoblast cell line) whole cell lysate at 1/35 dilution

Predicted band size: 67 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MT-ND5 conjugated to Keyhole Limpet Haemocyanin.

P03915

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MT-ND5 also known as NADH-ubiquinone oxidoreductase chain 5 is a subunit of mitochondrial complex I found in the inner mitochondrial membrane. This protein weighing approximately 74 kDa plays a central role in the electron transport chain facilitating the transfer of electrons from NADH to ubiquinone. Expression of MT-ND5 occurs ubiquitously in tissues with high energy demands such as the heart brain and skeletal muscle where efficient ATP production is essential.
Biological function summary

MT-ND5 functions as an integral component of mitochondrial complex I which is the first enzyme complex in the electron transport chain. By participating in this complex MT-ND5 contributes to the generation of a proton gradient across the inner mitochondrial membrane driving ATP synthesis through oxidative phosphorylation. Malfunction of MT-ND5 impairs energy production which can lead to a cascade of cellular energy deficiencies given its role within the complex.

Pathways

MT-ND5 associates with the oxidative phosphorylation pathway. It is important for maintaining aerobic energy production by linking NADH oxidation with ubiquinone reduction. Other proteins like MT-ND1 and MT-ND4L also participate in electron transport chain pathways working alongside MT-ND5 to ensure effective electron transfer and mitochondrial respiration. Its function within these pathways highlights its importance in metabolic regulation.

MT-ND5 mutations have associations with mitochondrial disorders such as Leigh syndrome and mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS). Defective MT-ND5 affects the electron transport chain leading to energy metabolism disruption in affected tissues. These disorders often involve other mitochondrial proteins such as MT-ND1 where combined mutations further exacerbate the dysfunction of mitochondrial capacity and highlight the profound impact on human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor (PubMed : 15250827). Essential for the catalytic activity and assembly of complex I (PubMed : 15250827).
See full target information MT-ND5
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Stem cells international 2022:5609501 PubMed36277041

2022

MFG-E8 Exerts Neuroprotection in Neural Stem Cells Induced by Anesthetic Sevoflurane via Regulating the PI3K/AKT Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Minmin Cai,Liufang Sheng

Cancer biology & therapy 23:1-8 PubMed36154909

2022

Targeting mitochondrial tyrosyl-tRNA synthetase YARS2 suppresses colorectal cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Qingxia Fang,Jingyang Lin,Liang Gao,Ruolang Pan,Xiaochun Zheng

Oxidative medicine and cellular longevity 2022:8038857 PubMed35087620

2022

Clinical Relevance and Tumor Growth Suppression of Mitochondrial ROS Regulators along NADH:Ubiquinone Oxidoreductase Subunit B3 in Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiao Zhu,Xiaobo Zheng,Dan Lu,Yun Zheng,Jun Liu
View all publications
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