AB233100

Anti-MT-ND5 antibody

Name: Anti-MT-ND5 antibody (ab233100) | Abcam
Brand: Abcam Limited
SKU: AB233100
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal MT-ND5 antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat NADH-ubiquinone oxidoreductase chain 5 aa 400-600.

View Alternative Names

Mtnd5, Nd5, NADH-ubiquinone oxidoreductase chain 5, NADH dehydrogenase subunit 5

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND5 antibody (AB233100)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND5 antibody (AB233100)

Paraffin-embedded rat brain tissue stained for MT-ND5 using ab233100 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND5 antibody (AB233100)

Paraffin-embedded rat spleen tissue stained for MT-ND5 using ab233100 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND5 antibody (AB233100)

Paraffin-embedded rat testis tissue stained for MT-ND5 using ab233100 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND5 antibody (AB233100)

Paraffin-embedded rat stomach tissue stained for MT-ND5 using ab233100 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND5 antibody (AB233100)

Paraffin-embedded rat kidney tissue stained for MT-ND5 using ab233100 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND5 antibody (AB233100)

Paraffin-embedded rat liver tissue stained for MT-ND5 using ab233100 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-MT-ND5 antibody (AB233100)

All lanes:

Western blot - Anti-MT-ND5 antibody (ab233100) at 3 µg/mL

Lane 1:

Rat serum

Lane 2:

Rat heart lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 67 kDa

false

Supplier Data

Western blot - Anti-MT-ND5 antibody (AB233100)

All lanes:

Western blot - Anti-MT-ND5 antibody (ab233100) at 3 µg/mL

All lanes:

Recombinant rat MT-ND5 protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 67 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Rat NADH-ubiquinone oxidoreductase chain 5 aa 400-600. The exact immunogen used to generate this antibody is proprietary information.

P11661

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab233100 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MT-ND5 also known as NADH-ubiquinone oxidoreductase chain 5 is a subunit of mitochondrial complex I found in the inner mitochondrial membrane. This protein weighing approximately 74 kDa plays a central role in the electron transport chain facilitating the transfer of electrons from NADH to ubiquinone. Expression of MT-ND5 occurs ubiquitously in tissues with high energy demands such as the heart brain and skeletal muscle where efficient ATP production is essential.

Biological function summary

MT-ND5 functions as an integral component of mitochondrial complex I which is the first enzyme complex in the electron transport chain. By participating in this complex MT-ND5 contributes to the generation of a proton gradient across the inner mitochondrial membrane driving ATP synthesis through oxidative phosphorylation. Malfunction of MT-ND5 impairs energy production which can lead to a cascade of cellular energy deficiencies given its role within the complex.

Pathways

MT-ND5 associates with the oxidative phosphorylation pathway. It is important for maintaining aerobic energy production by linking NADH oxidation with ubiquinone reduction. Other proteins like MT-ND1 and MT-ND4L also participate in electron transport chain pathways working alongside MT-ND5 to ensure effective electron transfer and mitochondrial respiration. Its function within these pathways highlights its importance in metabolic regulation.

MT-ND5 mutations have associations with mitochondrial disorders such as Leigh syndrome and mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS). Defective MT-ND5 affects the electron transport chain leading to energy metabolism disruption in affected tissues. These disorders often involve other mitochondrial proteins such as MT-ND1 where combined mutations further exacerbate the dysfunction of mitochondrial capacity and highlight the profound impact on human health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.

See full target information NADH-ubiquinone oxidoreductase chain 5

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:95 PubMed40089803

2025

ZEB1 promotes the immune escape of ovarian cancer through the MCSF-CCL18 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ping Jin,Guo-Wei Li,Qian-Qian Xu,Xiao-Lan Wang

Science advances 9:eadf2695 PubMed37058569

2023

A conditional knockout rat resource of mitochondrial protein-coding genes via a DdCBE-induced premature stop codon.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Tan,Xiaolong Qi,Weining Kong,Jiachuan Jin,Dan Lu,Xu Zhang,Yue Wang,Siting Wang,Wei Dong,Xudong Shi,Wei Chen,Jianying Wang,Keru Li,Yuan Xie,Lijuan Gao,Feifei Guan,Kai Gao,Chaojun Li,Cheng Wang,Zhibin Hu,Lianfeng Zhang,Xuejiang Guo,Bin Shen,Yuanwu Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MT-ND5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND5 antibody (AB233100)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND5 antibody (AB233100)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND5 antibody (AB233100)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND5 antibody (AB233100)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND5 antibody (AB233100)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT-ND5 antibody (AB233100)

Western blot - Anti-MT-ND5 antibody (AB233100)

Western blot - Anti-MT-ND5 antibody (AB233100)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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