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AB224060

Anti-MT3 antibody

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(1 Publication)

Rabbit Polyclonal MT3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Metallothionein-3 aa 1 to C-terminus.

View Alternative Names

Metallothionein-3, MT-3, GIFB, Growth inhibitory factor, Metallothionein-III, GIF, MT-III, MT3

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT3 antibody (AB224060)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT3 antibody (AB224060)

Immunohistochemistry analysis of human tonsil tissue labeling MT3 with ab224060 at 1 : 200 dilution. Negative control showing no positivity in cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT3 antibody (AB224060)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT3 antibody (AB224060)

Immunohistochemistry analysis of human pancreas tissue labeling MT3 with ab224060 at 1 : 200 dilution. Negative control showing no positive staining in cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT3 antibody (AB224060)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT3 antibody (AB224060)

Immunohistochemistry analysis of human cerebellum tissue labeling MT3 with ab224060 at 1 : 200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT3 antibody (AB224060)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT3 antibody (AB224060)

Immunohistochemistry analysis of human cerebral cortex tissue labeling MT3 with ab224060 at 1 : 200 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Metallothionein-3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P25713

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MT3 also known as metallothionein-3 is a small protein with a molecular mass of approximately 7 kDa. This protein belongs to the metallothionein family which is characterized by its high cysteine content and ability to bind heavy metals. MT3 is expressed mainly in the brain especially in neurons. Its structure allows it to regulate metal homeostasis and protect cells from damage caused by metal toxicity and oxidative stress.
Biological function summary

MT3 influences metal ion homeostasis and neuronal growth. Its role in regulating zinc and copper ions is significant for normal cellular function. MT3 interacts with other proteins to form complexes essential for neuronal differentiation and survival. It also exhibits neuroprotective properties modulating the effects of heavy metal ions and reducing reactive oxygen species which are particularly important in the central nervous system.

Pathways

The MT3 protein is critical in the regulation of metal ion pathways and oxidative stress response. It interacts with other metallothioneins like MT1 and MT2 which are involved in these pathways. MT3's participation in oxidative stress pathways highlights its role in modulating cellular responses to oxidative damage an important process in maintaining neuronal health and function.

MT3 has shown associations with Alzheimer's disease and Parkinson's disease. Changes in MT3 expression levels and its involvement with metal ion imbalance are linked to neurodegenerative processes. Additionally MT3 interacts with amyloid-beta a protein implicated in Alzheimer's disease suggesting a potential neuroprotective function. Furthermore MT3's regulation of metal ions might influence neurodegenerative pathways contributing to disease progression and providing targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro.
See full target information Metallothionein-3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 40:1295-1307 PubMed39698827

2024

TRIM22 mechanism promoting KAT2A ubiquitination degradation to regulate ferroptosis in hepatocellular carcinoma cell invasion and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Xiaoshan Chen,Wei Wei
View all publications
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