Rabbit Polyclonal MT3 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Metallothionein-3 aa 1 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Metallothionein-3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P25713
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Cynomolgus monkey | Predicted |
| Horse | Predicted |
| Pig | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Pig, Cynomolgus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro.
Alternative names
Metallothionein-3, MT-3, GIFB, Growth inhibitory factor, Metallothionein-III, GIF, MT-III, MT3
Recommended products
Rabbit Polyclonal MT3 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Metallothionein-3 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Metallothionein-3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P25713
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MT3 also known as metallothionein-3 is a small protein with a molecular mass of approximately 7 kDa. This protein belongs to the metallothionein family which is characterized by its high cysteine content and ability to bind heavy metals. MT3 is expressed mainly in the brain especially in neurons. Its structure allows it to regulate metal homeostasis and protect cells from damage caused by metal toxicity and oxidative stress.
- Biological function summary
MT3 influences metal ion homeostasis and neuronal growth. Its role in regulating zinc and copper ions is significant for normal cellular function. MT3 interacts with other proteins to form complexes essential for neuronal differentiation and survival. It also exhibits neuroprotective properties modulating the effects of heavy metal ions and reducing reactive oxygen species which are particularly important in the central nervous system.
- Pathways
The MT3 protein is critical in the regulation of metal ion pathways and oxidative stress response. It interacts with other metallothioneins like MT1 and MT2 which are involved in these pathways. MT3's participation in oxidative stress pathways highlights its role in modulating cellular responses to oxidative damage an important process in maintaining neuronal health and function.
- Associated diseases and disorders
MT3 has shown associations with Alzheimer's disease and Parkinson's disease. Changes in MT3 expression levels and its involvement with metal ion imbalance are linked to neurodegenerative processes. Additionally MT3 interacts with amyloid-beta a protein implicated in Alzheimer's disease suggesting a potential neuroprotective function. Furthermore MT3's regulation of metal ions might influence neurodegenerative pathways contributing to disease progression and providing targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT3 antibody (ab224060)
Immunohistochemistry analysis of human cerebellum tissue labeling MT3 with ab224060 at 1:200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT3 antibody (ab224060)
Immunohistochemistry analysis of human tonsil tissue labeling MT3 with ab224060 at 1:200 dilution. Negative control showing no positivity in cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT3 antibody (ab224060)
Immunohistochemistry analysis of human cerebral cortex tissue labeling MT3 with ab224060 at 1:200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT3 antibody (ab224060)
Immunohistochemistry analysis of human pancreas tissue labeling MT3 with ab224060 at 1:200 dilution. Negative control showing no positive staining in cells.
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