Anti-MT4-MMP antibody [EP1270Y]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MT4-MMP antibody [EP1270Y] (AB51075)

Immunofluorescent staining of HeLa cells using ab51075 (1 : 50).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT4-MMP antibody [EP1270Y] (AB51075)

ab51075 (1 : 50) staining human MT4-MMP in human lymphoma tissue by immunohistochemistry using paraffin embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Unknown

Western blot - Anti-MT4-MMP antibody [EP1270Y] (AB51075)

All lanes:

Western blot - Anti-MT4-MMP antibody [EP1270Y] (ab51075) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP labeled at 1/2000 dilution

Predicted band size: 67 kDa

Observed band size: 58 kDa

false

• IHC

CiteAb

Immunohistochemistry - Anti-MT4-MMP antibody [EP1270Y] (AB51075)

Immunohistochemistry-immunofluorescence using Anti-MT4-MMP antibody [EP1270Y], ab51075. Publication image from Andrés, V. et al., 2018, Nat Commun, 29500407. Legend direct from paper.

EnhancedαM integrin-dependent crawling of MT4-MMP-null patrolling monocytes on CCL2-inflamed endothelium. a Representative intravital microscopy images of CD115+/Ly6C– patrolling monocytes (CD115 in red and Ly6C in green) crawling on the CCL2-inflamed endothelium in the cremaster muscle of wild-type (MT4+/+) and MT4-MMP-null (MT4–/–) mice; the recording was performed in the presence of anti-Itgam blocking antibody (M1/70) or IgG isotype control. Arrowheads, arrows, and dots respectively indicate individual patrolling monocytes, blood flow, and monocyte trajectory. Time of recording is indicated. b The graph shows the numbers of crawling patrolling monocytes recorded in a in every venule from five independent mice per genotype and condition in two independent experiments. c Quantification of CD115+Ly6G- rolling (left) and adherent (right) monocytes in the CCL2-inflamed endothelium in the cremaster muscle of wild-type (MT4+/+) and MT4-MMP-null (MT4–/–) mice. n = 8 mice per genotype in two independent experiments. Data were tested by one-way ANOVA followed by Bonferroni’s post test in b and by two-tailed Student’s t-test in c. Results are expressed as mean ± SEM.*p < 0.05, **p < 0.01, and ***p < 0.001

• IHC

CiteAb

Immunohistochemistry - Anti-MT4-MMP antibody [EP1270Y] (AB51075)

Immunohistochemistry-immunofluorescence using Anti-MT4-MMP antibody [EP1270Y], ab51075. Publication image from Andrés, V. et al., 2018, Nat Commun, 29500407. Legend direct from paper.

Lack of MT4-MMP in BM-derived cells results in increased macrophage burden in atherosclerotic plaques and accelerated AT. a Representative images of Mac3 immunostaining (red; nuclei in blue) in transverse sections of aortas from Ldlr–/– mice transplanted with MT4-MMP+/+ (MT4+/+) or MT4-MMP–/– (MT4–/–) BM cells and fed a HFD for 8 weeks; scale bar, 20 µm. The right panel shows Mac3-positive cells quantified by Image J. n = 6 mice per genotype in two independent experiments. b Representative images of en face Oil Red-stained aortas from BM-transplanted Ldlr–/– and fed a HFD for 8 or 12 weeks (left) and graph shows the area (%) of Oil Red-positive lesions in the aortic arch (right); n = 6 and n = 16 mice per genotype for 8 and 12 weeks in two and three independent experiments, respectively. c Representative images of transverse sections of aortic sinus stained with H&E of BM-transplanted Ldlr–/– mice; scale bar, 200 µm. d Stary scoring (I–VI) of aortic lesions of BM-transplanted Ldlr–/– mice, shown as a percentage of all mice for each condition after feeding a HFD for 8 or 12 weeks; n = 6 and n = 16 mice per genotype and time point in two and three independent experiments. e Bar graphs show the percentage of BM-transplanted Ldlr–/– mice for each range of % of necrotic area (left) and fibrotic cap thickness (right) after 12 weeks of HFD; n = 16 mice per genotype in three independent experiments. Data were tested by two-tailed Student’s t-test in a, by two-way ANOVA followed by Bonferroni’s post test in b, and by χ2-test for a trend in d, e. Results are expressed as mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001