Rabbit Recombinant Monoclonal MT4-MMP antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse samples.
Images
![Western blot - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (AB247310), expandable thumbnail](https://content.abcam.com/products/images/mt4-mmp-antibody-ep1270y-bsa-and-azide-free-ab247310--western-blot-img56464.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (AB247310)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (AB247310), expandable thumbnail](https://content.abcam.com/products/images/mt4-mmp-antibody-ep1270y-bsa-and-azide-free-ab247310--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106348.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (AB247310)")
![Immunocytochemistry/ Immunofluorescence - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (AB247310), expandable thumbnail](https://content.abcam.com/products/images/mt4-mmp-antibody-ep1270y-bsa-and-azide-free-ab247310--immunocytochemistry-immunofluorescence-img58260.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (AB247310)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | WB | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Expected |
| Mouse | Expected | Expected | Not recommended | Not recommended | Tested |
| Rat | Predicted | Predicted | Not recommended | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Cleaves pro-TNF-alpha at the '74-Ala-|-Gln-75' site. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin.
Alternative names
MT4MMP, MMP17, Matrix metalloproteinase-17, MMP-17, Membrane-type matrix metalloproteinase 4, Membrane-type-4 matrix metalloproteinase, MT-MMP 4, MTMMP4, MT4-MMP
Recommended products
Rabbit Recombinant Monoclonal MT4-MMP antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1270Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247310 is the carrier-free version of Anti-MT4-MMP antibody [EP1270Y] ab51075.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MT4-MMP also known as matrix metalloproteinase 17 is a membrane-type metalloprotease. This protein has an approximate mass of 62 kDa and is anchored to the cell membrane by a GPI-anchor. MT4-MMP is expressed mainly in the brain and reproductive tissues suggesting its specialized roles across different regions. This protease belongs to the MMP family which consists of enzymes that can degrade extracellular matrix components and shows unique substrate specificities compared to other MMPs.
- Biological function summary
MT4-MMP functions in tissue remodeling and extracellular matrix degradation. It does not typically form a part of larger protein complexes but works independently or in association with other cell surface molecules. MT4-MMP breaks down specific substrates contributing to processes like development reproduction and repair mechanisms. Its activity regulates the availability of bioactive molecules and extracellular matrix components impacting cellular behavior and tissue structure.
- Pathways
MT4-MMP is involved in pathways critical for cell migration and angiogenesis. It participates in the activation of growth factors and modulates signaling pathways such as those related to integrins and vascular endothelial growth factor (VEGF). MT4-MMP associates with proteins like TIMP-2 which modulates its activity providing a regulatory mechanism within these pathways. This balance between activation and inhibition affects physiological and pathological tissue remodeling.
- Associated diseases and disorders
MT4-MMP has been implicated in cancer progression and arthritis. Its overexpression or dysregulation can lead to increased degradation of the extracellular matrix facilitating tumor invasion and metastasis. In arthritis its role in degrading connective tissue components contributes to joint destruction. MT4-MMP interacts with other MMPs and inhibitors like TIMP-2 influencing disease outcomes. Understanding these interactions can help in designing therapeutic strategies targeting MT4-MMP in such diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310), expandable thumbnail](https://content.abcam.com/products/images/mt4-mmp-antibody-ep1270y-bsa-and-azide-free-ab247310--western-blot-img56464.jpg "Western blot - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)")
Western blot - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)
This data was developed using Anti-MT4-MMP antibody [EP1270Y] ab51075, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-MT4-MMP antibody [EP1270Y] (Anti-MT4-MMP antibody [EP1270Y] ab51075) at 1/1000 dilution
All lanes: Mouse brain tissue lysate at 10 µg
SecondaryAll lanes: Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 67 kDa
Observed band size: 58 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310), expandable thumbnail](https://content.abcam.com/products/images/mt4-mmp-antibody-ep1270y-bsa-and-azide-free-ab247310--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106348.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)
This data was developed using Anti-MT4-MMP antibody [EP1270Y] ab51075, the same antibody clone in a different buffer formulation.Anti-MT4-MMP antibody [EP1270Y] ab51075 (1:50) staining human MT4-MMP in human lymphoma tissue by immunohistochemistry using paraffin embedded tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310), expandable thumbnail](https://content.abcam.com/products/images/mt4-mmp-antibody-ep1270y-bsa-and-azide-free-ab247310--immunocytochemistry-immunofluorescence-img58260.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)")
Immunocytochemistry/ Immunofluorescence - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (ab247310)
This data was developed using Anti-MT4-MMP antibody [EP1270Y] ab51075, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HeLa cells using Anti-MT4-MMP antibody [EP1270Y] ab51075 (1:50).
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