JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB247310

Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal MT4-MMP antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse samples.

View Alternative Names

MT4MMP, MMP17, Matrix metalloproteinase-17, MMP-17, Membrane-type matrix metalloproteinase 4, Membrane-type-4 matrix metalloproteinase, MT-MMP 4, MTMMP4, MT4-MMP

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (AB247310)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (AB247310)

This data was developed using ab51075, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HeLa cells using ab51075 (1 : 50).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (AB247310)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (AB247310)

This data was developed using ab51075, the same antibody clone in a different buffer formulation.

ab51075 (1 : 50) staining human MT4-MMP in human lymphoma tissue by immunohistochemistry using paraffin embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (AB247310)
  • WB

Unknown

Western blot - Anti-MT4-MMP antibody [EP1270Y] - BSA and Azide free (AB247310)

This data was developed using ab51075, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MT4-MMP antibody [EP1270Y] (<a href='/en-us/products/primary-antibodies/mt4-mmp-antibody-ep1270y-ab51075'>ab51075</a>) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP labeled at 1/2000 dilution

Predicted band size: 67 kDa

Observed band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1270Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB211926

Anti-TIMP1 antibody [EPR18352]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (AB211926)

  • 5
  • 10
Primary Antibodies

AB92536

Anti-MMP2 antibody [EPR1184]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-MMP2 antibody [EPR1184] (AB92536)

  • 4
  • 6
Primary Antibodies

AB51074

Anti-MMP14 antibody [EP1264Y]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-MMP14 antibody [EP1264Y] (AB51074)

  • 4
  • 20
Primary Antibodies

AB52631

Anti-MMP1 antibody [EP1247Y]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] (AB52631)

  • 5
  • 5
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column

Product details

ab247310 is the carrier-free version of ab51075.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MT4-MMP also known as matrix metalloproteinase 17 is a membrane-type metalloprotease. This protein has an approximate mass of 62 kDa and is anchored to the cell membrane by a GPI-anchor. MT4-MMP is expressed mainly in the brain and reproductive tissues suggesting its specialized roles across different regions. This protease belongs to the MMP family which consists of enzymes that can degrade extracellular matrix components and shows unique substrate specificities compared to other MMPs.
Biological function summary

MT4-MMP functions in tissue remodeling and extracellular matrix degradation. It does not typically form a part of larger protein complexes but works independently or in association with other cell surface molecules. MT4-MMP breaks down specific substrates contributing to processes like development reproduction and repair mechanisms. Its activity regulates the availability of bioactive molecules and extracellular matrix components impacting cellular behavior and tissue structure.

Pathways

MT4-MMP is involved in pathways critical for cell migration and angiogenesis. It participates in the activation of growth factors and modulates signaling pathways such as those related to integrins and vascular endothelial growth factor (VEGF). MT4-MMP associates with proteins like TIMP-2 which modulates its activity providing a regulatory mechanism within these pathways. This balance between activation and inhibition affects physiological and pathological tissue remodeling.

MT4-MMP has been implicated in cancer progression and arthritis. Its overexpression or dysregulation can lead to increased degradation of the extracellular matrix facilitating tumor invasion and metastasis. In arthritis its role in degrading connective tissue components contributes to joint destruction. MT4-MMP interacts with other MMPs and inhibitors like TIMP-2 influencing disease outcomes. Understanding these interactions can help in designing therapeutic strategies targeting MT4-MMP in such diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Cleaves pro-TNF at the '74-Ala-|-Gln-75' site. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin.
See full target information MMP17
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com