AB71153

Anti-MTA1 antibody

Name: Anti-MTA1 antibody (ab71153) | Abcam
Brand: Abcam Limited
SKU: AB71153
Price: 535 USD
Availability: InStock
Rating: 4 (10 reviews)

(10 Reviews)

(43 Publications)

Rabbit Polyclonal MTA1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 43 publications. Immunogen corresponding to Synthetic Peptide within Human MTA1.

View Alternative Names

Metastasis-associated protein MTA1, MTA1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA1 antibody (AB71153)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA1 antibody (AB71153)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human non-small cell lung cancer tissue labelling MTA1 with ab71153 at 1/1000 (0.2 µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-MTA1 antibody (AB71153)

Immunoprecipitation/ Western Blot of MTA1
Lane 1 : ab71153 at 3μg/mg whole cell lysate.
Lane 2 : Control IgG.

Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Subsequent WB detection was performed using 1 μg/ml ab71153.
Chemiluminescence with an exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-MTA1 antibody (ab71153)

Predicted band size: 81 kDa

false

Lab

Western blot - Anti-MTA1 antibody (AB71153)

False colour image of Western blot : Anti-MTA1 antibody staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab71153 was shown to bind specifically to MTA1. A band was observed at 80/85 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in Mta1 CRISPR-Cas9 edited cell line ab277164 (CRISPR-Cas9 edited cell lysate ab277206). The band observed in the CRISPR-Cas9 edited lysate lane below 80/85 kDa is likely to represent a truncated form of MTA1. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and Mta1 CRISPR-Cas9 edited HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-MTA1 antibody (ab71153) at 1/2000 dilution

Lane 1:

Wild-type HEK-293 cell lysate at 20 µg

Lane 2:

Mta1 CRISPR-Cas9 edited HEK-293 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

false

Unknown

Western blot - Anti-MTA1 antibody (AB71153)

All lanes:

Western blot - Anti-MTA1 antibody (ab71153) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HEK293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK whole cell lysate at 50 µg

Predicted band size: 81 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human MTA1. The exact immunogen used to generate this antibody is proprietary information.

Q13330

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MTA1 protein short for Metastasis-Associated 1 is a significant player in cellular mechanisms. It has an approximate molecular weight of 80 kDa. MTA1 is known for its function as a chromatin remodeling factor involved in altering chromatin structure to regulate gene expression. Alternate names include metastasis tumor antigen 1. MTA1 expresses widely but has higher levels in metastatic tumor cells playing a role in cancer progression by influencing gene transcription and chromatin dynamics.

Biological function summary

MTA1 contributes to regulating diverse cellular processes. It forms part of the NuRD complex which plays a critical role in mediating chromatin remodeling and histone deacetylation. This association enables MTA1 to influence key aspects of cellular behavior including cell proliferation differentiation and response to stress. The protein's involvement extends to modulating transcription factors and interacting with several other proteins impacting cellular growth and metastasis.

Pathways

MTA1 participates actively in the regulation of several key signaling networks. One significant pathway is the Wnt/β-catenin signaling pathway where MTA1 modulates gene expression by interacting with β-catenin. In another pathway it affects the estrogen receptor signaling pathway impacting gene transcription in hormone-dependent cancers. MTA1’s interaction with HDAC (Histone deacetylase) proteins emphasizes its role in epigenetic regulation across different biological pathways.

MTA1 is frequently connected with cancer progression and metastasis. Its overexpression correlates with poor prognosis in breast cancer highlighting its impact on tumor aggressiveness. Additionally MTA1 has implications in prostate cancer where it interacts with the androgen receptor influencing cancer cell proliferation and resistance to therapy. Understanding the role of MTA1 in these diseases could potentially lead to new therapeutic targets for cancer treatment.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcriptional coregulator which can act as both a transcriptional corepressor and coactivator (PubMed : 16617102, PubMed : 17671180, PubMed : 17922032, PubMed : 21965678, PubMed : 24413532). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 28977666). In the NuRD complex, regulates transcription of its targets by modifying the acetylation status of the target chromatin and cofactor accessibility to the target DNA (PubMed : 17671180). In conjunction with other components of NuRD, acts as a transcriptional corepressor of BRCA1, ESR1, TFF1 and CDKN1A (PubMed : 17922032, PubMed : 24413532). Acts as a transcriptional coactivator of BCAS3, and SUMO2, independent of the NuRD complex (PubMed : 16617102, PubMed : 17671180, PubMed : 21965678). Stimulates the expression of WNT1 by inhibiting the expression of its transcriptional corepressor SIX3 (By similarity). Regulates p53-dependent and -independent DNA repair processes following genotoxic stress (PubMed : 19837670). Regulates the stability and function of p53/TP53 by inhibiting its ubiquitination by COP1 and MDM2 thereby regulating the p53-dependent DNA repair (PubMed : 19837670). Plays a role in the regulation of the circadian clock and is essential for the generation and maintenance of circadian rhythms under constant light and for normal entrainment of behavior to light-dark (LD) cycles (By similarity). Positively regulates the CLOCK-BMAL1 heterodimer mediated transcriptional activation of its own transcription and the transcription of CRY1 (By similarity). Regulates deacetylation of BMAL1 by regulating SIRT1 expression, resulting in derepressing CRY1-mediated transcription repression (By similarity). With TFCP2L1, promotes establishment and maintenance of pluripotency in embryonic stem cells (ESCs) and inhibits endoderm differentiation (By similarity).. Isoform Short. Binds to ESR1 and sequesters it in the cytoplasm and enhances its non-genomic responses.

See full target information MTA1

style

left-column

Publications (43)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 12:5646-5656 PubMed36628289

2022

Interleukin-17 promotes the development of ovarian cancer through upregulation of MTA1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Na Guo,Jiawen Zhang

Neoplasma 69:1092-1100 PubMed35951452

2022

Correlation between metastasis-associated gene 1 expression and tumor-associated macrophages in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Ma,Yan-Xin Li,Qian-Qian Guo

Cell reports 38:110534 PubMed35320709

2022

The mTOR chromatin-bound interactome in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine R Dufour,Charlotte Scholtes,Ming Yan,Yonghong Chen,Lingwei Han,Ting Li,Hui Xia,Qiyun Deng,Mathieu Vernier,Vincent Giguère

Frontiers in oncology 12:825783 PubMed35350571

2022

Upregulation of MTA1 in Colon Cancer Drives A CD8 T Cell-Rich But Classical Macrophage-Lacking Immunosuppressive Tumor Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Yantong Zhou,Peng Nan,Chunxiao Li,Hongnan Mo,Ying Zhang,Haijuan Wang,Dongkui Xu,Fei Ma,Haili Qian

Mediators of inflammation 2021:6359652 PubMed34924813

2021

Ellagic Acid Alleviates Rheumatoid Arthritis in Rats through Inhibiting MTA1/HDAC1-Mediated Nur77 Deacetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Huanjin Song,Hao Wu,Jun Dong,Sihua Huang,Jintao Ye,Ruoxi Liu

Molecular medicine reports 25: PubMed34779499

2021

MTA1, a metastasis‑associated protein, in endothelial cells is an essential molecule for angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Mizuho Ishikawa,Mitsuhiko Osaki,Narumi Uno,Takahito Ohira,Hiroyuki Kugoh,Futoshi Okada

Cancer management and research 13:7087-7099 PubMed34531686

2021

TGF-β-MTA1-SMAD7-SMAD3-SOX4-EZH2 Signaling Axis Promotes Viability, Migration, Invasion and EMT of Hepatocellular Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kangjun Zhang,Taishi Fang,Yajie Shao,Yanhui Wu

Cell death and differentiation 28:2818-2836 PubMed33953349

2021

Snail/PRMT5/NuRD complex contributes to DNA hypermethylation in cervical cancer by TET1 inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Gao,Ruiqiong Liu,Dandan Feng,Wei Huang,Miaomiao Huo,Jingyao Zhang,Shuai Leng,Yang Yang,Tianshu Yang,Xin Yin,Xu Teng,Hefen Yu,Baowen Yuan,Yan Wang

Oncology reports 45: PubMed33786627

2021

Matrine inhibits ovarian cancer cell viability and promotes apoptosis by regulating the ERK/JNK signaling pathway via p38MAPK.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Liang,Jianxin Ju

Oncology letters 20:172 PubMed32934739

2020

Associations of MTA1 expression with CT features, pathology and prognosis of elderly patients with non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Yang,Chuanming Li,Xiao Han,Zhihua Feng,Feng Qiu,Junqing Han

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MTA1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA1 antibody (AB71153)

Immunoprecipitation - Anti-MTA1 antibody (AB71153)

Western blot - Anti-MTA1 antibody (AB71153)

Western blot - Anti-MTA1 antibody (AB71153)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (43)

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise