Rabbit Recombinant Monoclonal MTA1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
sELISA | |
---|---|
Human | Expected |
Mouse | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Transcriptional coregulator which can act as both a transcriptional corepressor and coactivator (PubMed:16617102, PubMed:17671180, PubMed:17922032, PubMed:21965678, PubMed:24413532). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:28977666). In the NuRD complex, regulates transcription of its targets by modifying the acetylation status of the target chromatin and cofactor accessibility to the target DNA (PubMed:17671180). In conjunction with other components of NuRD, acts as a transcriptional corepressor of BRCA1, ESR1, TFF1 and CDKN1A (PubMed:17922032, PubMed:24413532). Acts as a transcriptional coactivator of BCAS3, and SUMO2, independent of the NuRD complex (PubMed:16617102, PubMed:17671180, PubMed:21965678). Stimulates the expression of WNT1 by inhibiting the expression of its transcriptional corepressor SIX3 (By similarity). Regulates p53-dependent and -independent DNA repair processes following genotoxic stress (PubMed:19837670). Regulates the stability and function of p53/TP53 by inhibiting its ubiquitination by COP1 and MDM2 thereby regulating the p53-dependent DNA repair (PubMed:19837670). Plays a role in the regulation of the circadian clock and is essential for the generation and maintenance of circadian rhythms under constant light and for normal entrainment of behavior to light-dark (LD) cycles (By similarity). Positively regulates the CLOCK-BMAL1 heterodimer mediated transcriptional activation of its own transcription and the transcription of CRY1 (By similarity). Regulates deacetylation of BMAL1 by regulating SIRT1 expression, resulting in derepressing CRY1-mediated transcription repression (By similarity). With TFCP2L1, promotes establishment and maintenance of pluripotency in embryonic stem cells (ESCs) and inhibits endoderm differentiation (By similarity).Isoform ShortBinds to ESR1 and sequesters it in the cytoplasm and enhances its non-genomic responses.
Metastasis-associated protein MTA1, MTA1
Rabbit Recombinant Monoclonal MTA1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR24507-62
Affinity purification Protein A
Blue Ice
+4°C
+4°C
ab290684 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human and Mouse MTA1. The recommended pair for sandwich ELISA is:
Capture: ab290684, Human and Mouse MTA1 Capture Antibody (unconjugated)
Detector: Anti-MTA1 antibody [EPR24507-64] - BSA and Azide free (Detector) ab290685, Human and Mouse MTA1 Detector Antibody (unconjugated)
The reference range value is 0.156-10 ng/mL.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
The MTA1 protein short for Metastasis-Associated 1 is a significant player in cellular mechanisms. It has an approximate molecular weight of 80 kDa. MTA1 is known for its function as a chromatin remodeling factor involved in altering chromatin structure to regulate gene expression. Alternate names include metastasis tumor antigen 1. MTA1 expresses widely but has higher levels in metastatic tumor cells playing a role in cancer progression by influencing gene transcription and chromatin dynamics.
MTA1 contributes to regulating diverse cellular processes. It forms part of the NuRD complex which plays a critical role in mediating chromatin remodeling and histone deacetylation. This association enables MTA1 to influence key aspects of cellular behavior including cell proliferation differentiation and response to stress. The protein's involvement extends to modulating transcription factors and interacting with several other proteins impacting cellular growth and metastasis.
MTA1 participates actively in the regulation of several key signaling networks. One significant pathway is the Wnt/β-catenin signaling pathway where MTA1 modulates gene expression by interacting with β-catenin. In another pathway it affects the estrogen receptor signaling pathway impacting gene transcription in hormone-dependent cancers. MTA1’s interaction with HDAC (Histone deacetylase) proteins emphasizes its role in epigenetic regulation across different biological pathways.
MTA1 is frequently connected with cancer progression and metastasis. Its overexpression correlates with poor prognosis in breast cancer highlighting its impact on tumor aggressiveness. Additionally MTA1 has implications in prostate cancer where it interacts with the androgen receptor influencing cancer cell proliferation and resistance to therapy. Understanding the role of MTA1 in these diseases could potentially lead to new therapeutic targets for cancer treatment.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Sandwich ELISA of Human/Mouse MTA1 Antibody Pair - BSA and Azide free ab290707 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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