AB290684

Anti-MTA1 antibody [EPR24507-62] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal MTA1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Human samples.

View Alternative Names

Metastasis-associated protein MTA1, MTA1

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Sandwich ELISA - Anti-MTA1 antibody [EPR24507-62] - BSA and Azide free (Capture) (AB290684)

sELISA

Supplier Data

Sandwich ELISA - Anti-MTA1 antibody [EPR24507-62] - BSA and Azide free (Capture) (AB290684)

Sandwich ELISA of ab290707 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24507-62

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab290684 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human and Mouse MTA1. The recommended pair for sandwich ELISA is:
Capture: ab290684, Human and Mouse MTA1 Capture Antibody (unconjugated)
Detector: ab290685, Human and Mouse MTA1 Detector Antibody (unconjugated)
The reference range value is 0.156-10 ng/mL.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MTA1 protein short for Metastasis-Associated 1 is a significant player in cellular mechanisms. It has an approximate molecular weight of 80 kDa. MTA1 is known for its function as a chromatin remodeling factor involved in altering chromatin structure to regulate gene expression. Alternate names include metastasis tumor antigen 1. MTA1 expresses widely but has higher levels in metastatic tumor cells playing a role in cancer progression by influencing gene transcription and chromatin dynamics.

Biological function summary

MTA1 contributes to regulating diverse cellular processes. It forms part of the NuRD complex which plays a critical role in mediating chromatin remodeling and histone deacetylation. This association enables MTA1 to influence key aspects of cellular behavior including cell proliferation differentiation and response to stress. The protein's involvement extends to modulating transcription factors and interacting with several other proteins impacting cellular growth and metastasis.

Pathways

MTA1 participates actively in the regulation of several key signaling networks. One significant pathway is the Wnt/β-catenin signaling pathway where MTA1 modulates gene expression by interacting with β-catenin. In another pathway it affects the estrogen receptor signaling pathway impacting gene transcription in hormone-dependent cancers. MTA1’s interaction with HDAC (Histone deacetylase) proteins emphasizes its role in epigenetic regulation across different biological pathways.

MTA1 is frequently connected with cancer progression and metastasis. Its overexpression correlates with poor prognosis in breast cancer highlighting its impact on tumor aggressiveness. Additionally MTA1 has implications in prostate cancer where it interacts with the androgen receptor influencing cancer cell proliferation and resistance to therapy. Understanding the role of MTA1 in these diseases could potentially lead to new therapeutic targets for cancer treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional coregulator which can act as both a transcriptional corepressor and coactivator (PubMed : 16617102, PubMed : 17671180, PubMed : 17922032, PubMed : 21965678, PubMed : 24413532). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 28977666). In the NuRD complex, regulates transcription of its targets by modifying the acetylation status of the target chromatin and cofactor accessibility to the target DNA (PubMed : 17671180). In conjunction with other components of NuRD, acts as a transcriptional corepressor of BRCA1, ESR1, TFF1 and CDKN1A (PubMed : 17922032, PubMed : 24413532). Acts as a transcriptional coactivator of BCAS3, and SUMO2, independent of the NuRD complex (PubMed : 16617102, PubMed : 17671180, PubMed : 21965678). Stimulates the expression of WNT1 by inhibiting the expression of its transcriptional corepressor SIX3 (By similarity). Regulates p53-dependent and -independent DNA repair processes following genotoxic stress (PubMed : 19837670). Regulates the stability and function of p53/TP53 by inhibiting its ubiquitination by COP1 and MDM2 thereby regulating the p53-dependent DNA repair (PubMed : 19837670). Plays a role in the regulation of the circadian clock and is essential for the generation and maintenance of circadian rhythms under constant light and for normal entrainment of behavior to light-dark (LD) cycles (By similarity). Positively regulates the CLOCK-BMAL1 heterodimer mediated transcriptional activation of its own transcription and the transcription of CRY1 (By similarity). Regulates deacetylation of BMAL1 by regulating SIRT1 expression, resulting in derepressing CRY1-mediated transcription repression (By similarity). With TFCP2L1, promotes establishment and maintenance of pluripotency in embryonic stem cells (ESCs) and inhibits endoderm differentiation (By similarity).. Isoform Short. Binds to ESR1 and sequesters it in the cytoplasm and enhances its non-genomic responses.

See full target information MTA1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MTA1 antibody [EPR24507-62] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-MTA1 antibody [EPR24507-62] - BSA and Azide free (Capture) (AB290684)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise