Rabbit Polyclonal MTA3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human MTA3 aa 400-450.
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Publications
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- Journal of cellular and molecular medicine 24:5998-60072020Metastasis-associated protein 1 promotes epithelial-mesenchymal transition in idiopathic pulmonary fibrosis by up-regulating Snail expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeibin Qian et. al.PubMed 32187849
- AIMS medical science 4:17-272017MTA3 Regulates Extravillous Trophoblast Invasion Through NuRD Complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Chen,Sok Kean Khoo,Richard Leach,Kai WangPubMed 28959722
- Oncotarget 8:2916-29352016HIC1 (hypermethylated in cancer 1) SUMOylation is dispensable for DNA repair but is essential for the apoptotic DNA damage response (DDR) to irreparable DNA double-strand breaks (DSBs).Applications:WBReactive species:Unspecified reactive speciesSonia Paget,Marion Dubuissez,Vanessa Dehennaut,Joe Nassour,Brennan T Harmon,Nathalie Spruyt,Ingrid Loison,Corinne Abbadie,Brian R Rood,Dominique LeprincePubMed 27935866
- Molecular and cellular biology 36:1881-982016Protein Kinase C-Mediated Phosphorylation of BCL11B at Serine 2 Negatively Regulates Its Interaction with NuRD Complexes during CD4+ T-Cell Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarion Dubuissez et. al.PubMed 27161321
- Oncology reports 30:745-502013β-elemene decreases cell invasion by upregulating E-cadherin expression in MCF-7 human breast cancer cells.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MTA3 aa 400-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BTC8
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Chimpanzee | Predicted |
| Orangutan | Predicted |
| Rhesus monkey | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:12705869, PubMed:16428440, PubMed:28977666). Plays a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels (PubMed:12705869). Contributes to transcriptional repression by BCL6 (PubMed:15454082).
Alternative names
KIAA1266, MTA3, Metastasis-associated protein MTA3
Recommended products
Rabbit Polyclonal MTA3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human MTA3 aa 400-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MTA3 aa 400-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BTC8
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
MTA3 antibody was affinity purified using an epitope specific to MTA3 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Concentration is optimized for IHC and not determined
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The MTA3 protein also known as Metastasis-Associated Protein 3 is a member of the MTA family and has a molecular mass of approximately 53 kDa. MTA3 localizes in the nucleus and shows expression in various tissues including breast tissue and the immune system. It functions as a transcriptional corepressor particularly influencing E-cadherin repression by being part of the nucleosome remodeling and histone deacetylase (NuRD) complex.
- Biological function summary
Activities involving gene expression regulation occur through interactions with other chromatin-associated molecules. MTA3 participates as a component of the NuRD complex which also includes Mi2 and HDAC1 that is critical in chromatin remodeling and transcriptional repression. Through MTA3's involvement the complex influences cell proliferation differentiation and epithelial-mesenchymal transition (EMT).
- Pathways
MTA3 is particularly significant in the EMT pathway affecting cell migration and invasion capacities. It interacts with proteins such as TWIST and ZEB1 which are associated with EMT. Additionally MTA3 plays a role in the Notch signaling pathway where it helps in maintaining proper cell lineage differentiation by modulating transcription factors through deacetylation processes.
- Associated diseases and disorders
Altered MTA3 expression is seen in breast cancer and has a link to its progression. It connects closely with E-cadherin which serves as a marker for EMT and cancer metastasis. MTA3's involvement in histone modification pathways also correlates with altered gene expression seen in various autoimmune disorders making it a possible point of interest for therapeutic research and interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA3 antibody (ab87275)
ab87275 at 1/250 dilution staining MTA3 in Human breast carcinoma by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTA3 antibody (ab87275)
ab87275 at 1/250 dilution staining MTA3 in Human seminoma by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining
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