AB176346

Anti-MTA3 antibody [EPR12777(B)]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal MTA3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

View Alternative Names

KIAA1266, MTA3, Metastasis-associated protein MTA3

3 Images

Flow Cytometry (Intracellular) - Anti-MTA3 antibody [EPR12777(B)] (AB176346)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MTA3 antibody [EPR12777(B)] (AB176346)

Intracellular flow cytometric analysis of permeabilized Ramos cells labeling MTA3with ab176346 at 1/10 dilution (red)or a rabbit IgG (negative) (green).

Supplier Data

Western blot - Anti-MTA3 antibody [EPR12777(B)] (AB176346)

All lanes:

Western blot - Anti-MTA3 antibody [EPR12777(B)] (ab176346) at 1/1000 dilution

Lane 1:

T47D cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

Ramos cell lysate at 10 µg

Predicted band size: 68 kDa

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CiteAb

Western blot - Anti-MTA3 antibody [EPR12777(B)] (AB176346)

MTA3 western blot using anti-MTA3 antibody [EPR12777(B)] ab176346. Publication image and figure legend from Xiaomeng, F., Lei, L., et al., 2020, Braz J Med Biol Res, PubMed 32401925.

ab176346 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab176346 please see the product overview.

Effect of β-elemene+paclitaxel on migration (A) andinvasion (B) of SKOV3 cells (magnification bar, 100 μm).C and D, Relative E-cadherin and MTA3 mRNA(C) and protein (D) expression in SKOV3cells after treatment with β-elemene and/or paclitaxel. Data arereported as means±SD. **p<0.01 (ANOVA followed by Tukey's multiplecomparison post hoc test). ns : not significant.

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Carrier free

Anti-MTA3 antibody [EPR12777(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12777(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MTA3 protein also known as Metastasis-Associated Protein 3 is a member of the MTA family and has a molecular mass of approximately 53 kDa. MTA3 localizes in the nucleus and shows expression in various tissues including breast tissue and the immune system. It functions as a transcriptional corepressor particularly influencing E-cadherin repression by being part of the nucleosome remodeling and histone deacetylase (NuRD) complex.

Biological function summary

Activities involving gene expression regulation occur through interactions with other chromatin-associated molecules. MTA3 participates as a component of the NuRD complex which also includes Mi2 and HDAC1 that is critical in chromatin remodeling and transcriptional repression. Through MTA3's involvement the complex influences cell proliferation differentiation and epithelial-mesenchymal transition (EMT).

Pathways

MTA3 is particularly significant in the EMT pathway affecting cell migration and invasion capacities. It interacts with proteins such as TWIST and ZEB1 which are associated with EMT. Additionally MTA3 plays a role in the Notch signaling pathway where it helps in maintaining proper cell lineage differentiation by modulating transcription factors through deacetylation processes.

Altered MTA3 expression is seen in breast cancer and has a link to its progression. It connects closely with E-cadherin which serves as a marker for EMT and cancer metastasis. MTA3's involvement in histone modification pathways also correlates with altered gene expression seen in various autoimmune disorders making it a possible point of interest for therapeutic research and interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 12705869, PubMed : 16428440, PubMed : 28977666). Plays a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels (PubMed : 12705869). Contributes to transcriptional repression by BCL6 (PubMed : 15454082).

See full target information MTA3

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 299:104662 PubMed36997086

2023

The stem cell-supporting small molecule UM171 triggers Cul3-KBTBD4-mediated degradation of ELM2 domain-harboring proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Kristijonas Žemaitis,Sudip Ghosh,Jenny Hansson,Agatheeswaran Subramaniam

Brazilian journal of medical and biological resear 53:e8885 PubMed32401925

2020

Treatment with β-elemene combined with paclitaxel inhibits growth, migration, and invasion and induces apoptosis of ovarian cancer cells by activation of STAT-NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fu Xiaomeng,Lv Lei,An Jinghong,Jiang Juan,Yue Qi,Yuan Dandan

Molecular medicine reports 19:3190-3200 PubMed30816482

2019

High glucose promotes hepatic fibrosis via miR‑32/MTA3‑mediated epithelial‑to‑mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Li,Zhange Li,Yuan Lin,Hui Che,Yingying Hu,Xujuan Kang,Ying Zhang,Lihong Wang,Yong Zhang

Oncology letters 16:2143-2150 PubMed30008912

2018

β-elemene enhances anticancer bone neoplasms efficacy of paclitaxel through regulation of GPR124 in bone neoplasms cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zongze Wang,Ying Li,Fengxin Zhou,Zhe Piao,Jian Hao

Autophagy 11:2309-22 PubMed26735435

2016

Epigenetic regulation of autophagy by the methyltransferase EZH2 through an MTOR-dependent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fu-Zheng Wei,Ziyang Cao,Xi Wang,Hui Wang,Mu-Yan Cai,Tingting Li,Naoko Hattori,Donglai Wang,Yipeng Du,Boyan Song,Lin-Lin Cao,Changchun Shen,Lina Wang,Haiying Wang,Yang Yang,Dan Xie,Fan Wang,Toshikazu Ushijima,Ying Zhao,Wei-Guo Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MTA3 antibody [EPR12777(B)]

Flow Cytometry (Intracellular) - Anti-MTA3 antibody [EPR12777(B)] (AB176346)

Western blot - Anti-MTA3 antibody [EPR12777(B)] (AB176346)

Western blot - Anti-MTA3 antibody [EPR12777(B)] (AB176346)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise