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AB55517

Anti-MTAP antibody [2G4]

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(4 Publications)

Anti-MTAP antibody [2G4] (ab55517) is a mouse monoclonal antibody detecting MTAP in Western Blot, Flow Cytometry, ICC/IF. Suitable for Human.

- Trusted since 2007

View Alternative Names

MSAP, MTAP, S-methyl-5'-thioadenosine phosphorylase, 5'-methylthioadenosine phosphorylase, MTA phosphorylase, MTAPase

3 Images
Western blot - Anti-MTAP antibody [2G4] (AB55517)
  • WB

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Western blot - Anti-MTAP antibody [2G4] (AB55517)

MTAP antibody (ab55517) at 1 μg/lane + HeLa cell lysate at 25 μg/lane.

This image was genrated using the ascites version of the product.

All lanes:

Western blot - Anti-MTAP antibody [2G4] (ab55517)

Predicted band size: 31 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-MTAP antibody [2G4] (AB55517)
  • ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MTAP antibody [2G4] (AB55517)

ab55517 at 10 μg/ml staining MTAP in human Hella cells by Immunocytochemistry/ Immunofluorescence.

This image was genrated using the ascites version of the product.

Flow Cytometry - Anti-MTAP antibody [2G4] (AB55517)
  • Flow Cyt

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Flow Cytometry - Anti-MTAP antibody [2G4] (AB55517)

Overlay histogram showing HeLa cells stained with ab55517 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55517, 2μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
This image was genrated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2G4

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MTAP.

Q13126

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Reactivity data

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Product details

What is this antibody validated in?
Anti-MTAP antibody [2G4] (ab55517) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of MTAP?
Anti-MTAP [2G4] (ab55517) specifically detects a band for MTAP (UniProt: Q13126) at a molecular weight of 31kDa.
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MTAP also known as methylthioadenosine phosphorylase is an essential enzyme that breaks down 5'-methylthioadenosine (MTA) a byproduct of polyamine synthesis. MTAP has a molecular weight of about 31 kDa. The enzyme converts MTA into adenine and 5-methylthioribose-1-phosphate which re-enter the methionine and adenine salvage pathways. MTAP is widely expressed in most tissues but its activity is especially high in the liver and kidney. Alternative names for MTAP include 2G4 and MTAP-A.
Biological function summary

Methylthioadenosine phosphorylase plays a significant role in the salvage pathways for methionine and adenine critical for cellular growth and proliferation. MTAP operates as a part of a complex metabolic network involved in polyamine metabolism. In addition to its metabolic functions MTAP contributes to the regulation of the immune response and cell cycle. MTAP immunohistochemistry is often used to study its expression patterns in various tissues.

Pathways

Methylthioadenosine phosphorylase participates importantly in the polyamine biosynthesis and methionine salvage pathways. These pathways are integral for maintaining cellular homeostasis and nucleotide pools. MTAP works closely with proteins such as methionine adenosyltransferase (MAT) and adenosylmethionine decarboxylase (AMD). In concert they facilitate the regeneration of methionine highlighting MTAP's role in cellular adaptation to metabolic demands.

Methylthioadenosine phosphorylase deficiency or deletion is linked to certain cancers such as gliomas and lymphomas. Loss of MTAP function is often associated with co-deletion of the tumor suppressor protein p16INK4a observed in various malignancies. This deletion can lead to an accumulation of MTA creating a toxic environment that promotes cancer cell proliferation. MTAP and its interaction with proteins like p16INK4a highlight its relevance as a potential target for therapeutic intervention in cancer treatment programs.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.
See full target information MTAP
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer discovery 13:2412-2431 PubMed37552839

2023

MRTX1719 Is an MTA-Cooperative PRMT5 Inhibitor That Exhibits Synthetic Lethality in Preclinical Models and Patients with MTAP-Deleted Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lars D Engstrom,Ruth Aranda,Laura Waters,Krystal Moya,Vickie Bowcut,Laura Vegar,David Trinh,Allan Hebbert,Christopher R Smith,Svitlana Kulyk,J David Lawson,Leo He,Laura D Hover,Julio Fernandez-Banet,Jill Hallin,Darin Vanderpool,David M Briere,Alice Blaj,Matthew A Marx,Jordi Rodon,Michael Offin,Kathryn C Arbour,Melissa L Johnson,David J Kwiatkowski,Pasi A Jänne,Candace L Haddox,Kyriakos P Papadopoulos,Jason T Henry,Konstantinos Leventakos,James G Christensen,Ronald Shazer,Peter Olson

PloS one 11:e0160348 PubMed27479139

2016

Characterization of the Methylthioadenosine Phosphorylase Polymorphism rs7023954 - Incidence and Effects on Enzymatic Function in Malignant Melanoma.

Applications

WB

Species

Unspecified reactive species

Katharina Limm,Katja Dettmer,Jörg Reinders,Peter J Oefner,Anja-Katrin Bosserhoff

The Journal of investigative dermatology 136:1238-1246 PubMed26872600

2016

Independent Loss of Methylthioadenosine Phosphorylase (MTAP) in Primary Cutaneous T-Cell Lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wesley J Woollard,Nithyha P Kalaivani,Christine L Jones,Catherine Roper,Lam Tung,Jae Jin Lee,Bjorn R Thomas,Isabella Tosi,Silvia Ferreira,Carl Z Beyers,Robert C T McKenzie,Rosie M Butler,Anna Lorenc,Sean J Whittaker,Tracey J Mitchell

PloS one 8:e80703 PubMed24324622

2013

Expression and function of methylthioadenosine phosphorylase in chronic liver disease.

Applications

IHC-P, WB

Species

Human, Human

Barbara Czech,Katja Dettmer,Daniela Valletta,Michael Saugspier,Andreas Koch,Axel P Stevens,Wolfgang E Thasler,Martina Müller,Peter J Oefner,Anja-Katrin Bosserhoff,Claus Hellerbrand
View all publications
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