Rabbit Recombinant Monoclonal MTAP antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples.
Images
![Western blot - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (AB256356), expandable thumbnail](https://content.abcam.com/products/images/mtap-antibody-epr22570-76-bsa-and-azide-free-ab256356--western-blot-img2084.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (AB256356)")
![Flow Cytometry (Intracellular) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (AB256356), expandable thumbnail](https://content.abcam.com/products/images/mtap-antibody-epr22570-76-bsa-and-azide-free-ab256356--flow-cytometry-intracellular-img37284.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (AB256356)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (AB256356), expandable thumbnail](https://content.abcam.com/products/images/mtap-antibody-epr22570-76-bsa-and-azide-free-ab256356--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img64072.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (AB256356)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (AB256356), expandable thumbnail](https://content.abcam.com/products/images/mtap-antibody-epr22570-76-bsa-and-azide-free-ab256356--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123514.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (AB256356)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (AB256356), expandable thumbnail](https://content.abcam.com/products/images/mtap-antibody-epr22570-76-bsa-and-azide-free-ab256356--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img69402.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (AB256356)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Tested | Expected | Expected | Tested |
| Rat | Not recommended | Tested | Expected | Expected | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
8 products for Alternative Product
5 products for Alternative Version
Target data
Function
Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.
Alternative names
S-methyl-5'-thioadenosine phosphorylase, 5'-methylthioadenosine phosphorylase, MTA phosphorylase, MTAP, MTAPase, MTAP, MSAP
Recommended products
Rabbit Recombinant Monoclonal MTAP antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples.
Alternative names
S-methyl-5'-thioadenosine phosphorylase, 5'-methylthioadenosine phosphorylase, MTA phosphorylase, MTAP, MTAPase, MTAP, MSAP
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR22570-76
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab256356 is the carrier-free version of Anti-MTAP antibody [EPR22570-76] ab254265.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MTAP also known as methylthioadenosine phosphorylase is an essential enzyme that breaks down 5'-methylthioadenosine (MTA) a byproduct of polyamine synthesis. MTAP has a molecular weight of about 31 kDa. The enzyme converts MTA into adenine and 5-methylthioribose-1-phosphate which re-enter the methionine and adenine salvage pathways. MTAP is widely expressed in most tissues but its activity is especially high in the liver and kidney. Alternative names for MTAP include 2G4 and MTAP-A.
- Biological function summary
Methylthioadenosine phosphorylase plays a significant role in the salvage pathways for methionine and adenine critical for cellular growth and proliferation. MTAP operates as a part of a complex metabolic network involved in polyamine metabolism. In addition to its metabolic functions MTAP contributes to the regulation of the immune response and cell cycle. MTAP immunohistochemistry is often used to study its expression patterns in various tissues.
- Pathways
Methylthioadenosine phosphorylase participates importantly in the polyamine biosynthesis and methionine salvage pathways. These pathways are integral for maintaining cellular homeostasis and nucleotide pools. MTAP works closely with proteins such as methionine adenosyltransferase (MAT) and adenosylmethionine decarboxylase (AMD). In concert they facilitate the regeneration of methionine highlighting MTAP's role in cellular adaptation to metabolic demands.
- Associated diseases and disorders
Methylthioadenosine phosphorylase deficiency or deletion is linked to certain cancers such as gliomas and lymphomas. Loss of MTAP function is often associated with co-deletion of the tumor suppressor protein p16INK4a observed in various malignancies. This deletion can lead to an accumulation of MTA creating a toxic environment that promotes cancer cell proliferation. MTAP and its interaction with proteins like p16INK4a highlight its relevance as a potential target for therapeutic intervention in cancer treatment programs.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356), expandable thumbnail](https://content.abcam.com/products/images/mtap-antibody-epr22570-76-bsa-and-azide-free-ab256356--western-blot-img2084.jpg "Western blot - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356)")
Western blot - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356)
This data was developed using the same antibody clone in a different buffer formulation (Anti-MTAP antibody [EPR22570-76] ab254265).
Lanes 1-4: Merged signal (red and green). Green - Anti-MTAP antibody [EPR22570-76] ab254265 observed at 32 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-MTAP antibody [EPR22570-76] ab254265 Anti-MTAP antibody [EPR22570-76] was shown to specifically react with MTAP in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human MTAP knockout HeLa cell line ab265272 (knockout cell lysate Human MTAP knockout HeLa cell lysate ab257194) was used. Wild-type and MTAP knockout samples were subjected to SDS-PAGE. Anti-MTAP antibody [EPR22570-76] ab254265 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-MTAP antibody [EPR22570-76] (Anti-MTAP antibody [EPR22570-76] ab254265) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: MTAP knockout HeLa cell lysate at 20 µg
Lane 3: HT-29 cell lysate at 20 µg
Lane 4: A549 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 32 kDa
![Flow Cytometry (Intracellular) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356), expandable thumbnail](https://content.abcam.com/products/images/mtap-antibody-epr22570-76-bsa-and-azide-free-ab256356--flow-cytometry-intracellular-img37284.jpg "Flow Cytometry (Intracellular) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356)")
Flow Cytometry (Intracellular) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A549 (human lung carcinoma cell line) (Left) anf H-29 (human colorectal adenocarcinoma cell line) (Right) cells labeling MTAP with Anti-MTAP antibody [EPR22570-76] ab254265 at 1/400 dilution (red) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
Negative control: A549?PMID: 8971171.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MTAP antibody [EPR22570-76] ab254265).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356), expandable thumbnail](https://content.abcam.com/products/images/mtap-antibody-epr22570-76-bsa-and-azide-free-ab256356--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img64072.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling MTAP with Anti-MTAP antibody [EPR22570-76] ab254265 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in rat liver (PMID:15492751) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MTAP antibody [EPR22570-76] ab254265).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356), expandable thumbnail](https://content.abcam.com/products/images/mtap-antibody-epr22570-76-bsa-and-azide-free-ab256356--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123514.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling MTAP with Anti-MTAP antibody [EPR22570-76] ab254265 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in mouse liver (PMID:15492751) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MTAP antibody [EPR22570-76] ab254265).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356), expandable thumbnail](https://content.abcam.com/products/images/mtap-antibody-epr22570-76-bsa-and-azide-free-ab256356--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img69402.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356)
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling MTAP with Anti-MTAP antibody [EPR22570-76] ab254265 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in stroma cells and no staining in tumor cells of human bladder cancer (PMID:27270441) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MTAP antibody [EPR22570-76] ab254265).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356), expandable thumbnail](https://content.abcam.com/products/images/mtap-antibody-epr22570-76-bsa-and-azide-free-ab256356--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42777.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356)
Immunohistochemical analysis of paraffin-embedded human breast tissue labeling MTAP with Anti-MTAP antibody [EPR22570-76] ab254265 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human breast (PMID:26751376, 18712977) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MTAP antibody [EPR22570-76] ab254265).
![Immunocytochemistry/ Immunofluorescence - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356), expandable thumbnail](https://content.abcam.com/products/images/mtap-antibody-epr22570-76-bsa-and-azide-free-ab256356--immunocytochemistry-immunofluorescence-img123243.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356)")
Immunocytochemistry/ Immunofluorescence - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma cell line) and HT-29 (human colorectal adenocarcinoma cell line) cells labeling MTAP with Anti-MTAP antibody [EPR22570-76] ab254265 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining in HT-29 cell line. The nuclear counter stain is DAPI (blue) Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
SEcondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
Negative control: A549?PMID: 8971171.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MTAP antibody [EPR22570-76] ab254265).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356), expandable thumbnail](https://content.abcam.com/products/images/mtap-antibody-epr22570-76-bsa-and-azide-free-ab256356--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42777.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356)")
![Immunocytochemistry/ Immunofluorescence - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356), expandable thumbnail](https://content.abcam.com/products/images/mtap-antibody-epr22570-76-bsa-and-azide-free-ab256356--immunocytochemistry-immunofluorescence-img123243.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MTAP antibody [EPR22570-76] - BSA and Azide free (ab256356)")
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