JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB238024

Anti-MTAP antibody [MTAP/1813]

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal MTAP antibody. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MTAP aa 50-200.

View Alternative Names

MSAP, MTAP, S-methyl-5'-thioadenosine phosphorylase, 5'-methylthioadenosine phosphorylase, MTA phosphorylase, MTAPase

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [MTAP/1813] (AB238024)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [MTAP/1813] (AB238024)

Formalin-fixed, paraffin-embedded human kidney tissue stained for MTAP using ab238024 at 2 μg/ml in immunohistochemical analysis.

Protein Array - Anti-MTAP antibody [MTAP/1813] (AB238024)
  • Protein Array

Unknown

Protein Array - Anti-MTAP antibody [MTAP/1813] (AB238024)

ab238024 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Western blot - Anti-MTAP antibody [MTAP/1813] (AB238024)
  • WB

Supplier Data

Western blot - Anti-MTAP antibody [MTAP/1813] (AB238024)

All lanes:

Western blot - Anti-MTAP antibody [MTAP/1813] (ab238024) at 2 µg/mL

All lanes:

A431 (human epidermoid carcinoma cell line) cell lysate

Predicted band size: 31 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MTAP/1813

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB, Protein Array, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MTAP aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q13126

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Incubate with primary antibody for 30 minutes at RT.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MTAP also known as methylthioadenosine phosphorylase is an essential enzyme that breaks down 5'-methylthioadenosine (MTA) a byproduct of polyamine synthesis. MTAP has a molecular weight of about 31 kDa. The enzyme converts MTA into adenine and 5-methylthioribose-1-phosphate which re-enter the methionine and adenine salvage pathways. MTAP is widely expressed in most tissues but its activity is especially high in the liver and kidney. Alternative names for MTAP include 2G4 and MTAP-A.
Biological function summary

Methylthioadenosine phosphorylase plays a significant role in the salvage pathways for methionine and adenine critical for cellular growth and proliferation. MTAP operates as a part of a complex metabolic network involved in polyamine metabolism. In addition to its metabolic functions MTAP contributes to the regulation of the immune response and cell cycle. MTAP immunohistochemistry is often used to study its expression patterns in various tissues.

Pathways

Methylthioadenosine phosphorylase participates importantly in the polyamine biosynthesis and methionine salvage pathways. These pathways are integral for maintaining cellular homeostasis and nucleotide pools. MTAP works closely with proteins such as methionine adenosyltransferase (MAT) and adenosylmethionine decarboxylase (AMD). In concert they facilitate the regeneration of methionine highlighting MTAP's role in cellular adaptation to metabolic demands.

Methylthioadenosine phosphorylase deficiency or deletion is linked to certain cancers such as gliomas and lymphomas. Loss of MTAP function is often associated with co-deletion of the tumor suppressor protein p16INK4a observed in various malignancies. This deletion can lead to an accumulation of MTA creating a toxic environment that promotes cancer cell proliferation. MTAP and its interaction with proteins like p16INK4a highlight its relevance as a potential target for therapeutic intervention in cancer treatment programs.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.
See full target information MTAP
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal, genetic engineering & biotechnology 23:100496 PubMed40390503

2025

Co-expression of HIF1A with multi-drug transporters (P-GP, MRP1, and BCRP) in chemoresistant breast, colorectal, and ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sudipta Deb Nath,Md Tamzid Hossain Tanim,Md Mahmudul Hasan Akash,Mohammad Golam Mostafa,Abu Ashfaqur Sajib
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB96231

Anti-MTAP antibody

Immunocytochemistry/ Immunofluorescence - Anti-MTAP antibody (AB96231)

  • 4
  • 1
Primary Antibodies

AB55517

Anti-MTAP antibody [2G4]

Western blot - Anti-MTAP antibody [2G4] (AB55517)

  • 0
  • 0
Primary Antibodies

AB225149

PE Anti-MTAP antibody [EPR6893]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-MTAP antibody [EPR6893] (AB225149)

  • 0
  • 0
Primary Antibodies

AB126770

Anti-MTAP antibody [EPR6893]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR6893] (AB126770)

  • 3
  • 1
Primary Antibodies

AB126623

Anti-MTAP antibody [EPR6892]

RabMAb|Recombinant

Western blot - Anti-MTAP antibody [EPR6892] (AB126623)

  • 5
  • 1
Primary Antibodies

AB225148

Alexa Fluor® 488 Anti-MTAP antibody [EPR6893]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-MTAP antibody [EPR6893] (AB225148)

  • 0
  • 0
Primary Antibodies

AB232417

Anti-MTAP antibody [EPR6893] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTAP antibody [EPR6893] - BSA and Azide free (AB232417)

  • 0
  • 0

Complementary Products

Assay Kits

AB211094

Adenosine Assay Kit (Fluorometric)

Functional Studies - Adenosine Assay Kit (Fluorometric) (AB211094)

  • 4
  • 3
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB255611

Anti-BAP1 antibody [EPR22826-65]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (AB255611)

  • 0
  • 0
Primary Antibodies

AB214488

Anti-TROP2 antibody [EPR20043]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant

Western blot - Anti-TROP2 antibody [EPR20043] (AB214488)

  • 5
  • 9

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com