Rabbit Polyclonal MTBP antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MTBP aa 100-150 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Animal cells and systems 25:152-1602021MTBP promoted the proliferation, migration and invasion of colon cancer cells by activating the expression of ZEB2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaerhati Shayimu,Aikeremu Yusufu,Aizimaiti Rehemutula,Darebai Redati,Rexida Jiapaer,Rousidan TuerdiPubMed 34262658
- Theranostics 9:6019-60302019MTBP regulates cell survival and therapeutic sensitivity in TP53 wildtype glioblastomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifu Song et. al.PubMed 31534534
- Oncology letters 14:6409-64162017MDM2 binding protein as a predictor of metastasis and a novel prognostic biomarker in patients with gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wang et. al.PubMed 29422956
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MTBP aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q96DY7
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Cow | Predicted |
| Pig | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Pig | Dilution info - | Notes - |
Target data
Function
Inhibits cell migration in vitro and suppresses the invasive behavior of tumor cells (By similarity). May play a role in MDM2-dependent p53/TP53 homeostasis in unstressed cells. Inhibits autoubiquitination of MDM2, thereby enhancing MDM2 stability. This promotes MDM2-mediated ubiquitination of p53/TP53 and its subsequent degradation.
Alternative names
Mdm2-binding protein, hMTBP, MTBP
Recommended products
Rabbit Polyclonal MTBP antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MTBP aa 100-150 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MTBP aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q96DY7
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MTBP also known as Mdm2-binding protein interacts mechanically with the Mdm2 protein. It has a molecular mass of approximately 125 kDa. MTBP expresses in many tissues with high levels notably in the brain and testes. The expression pattern suggests diverse roles in cellular functioning and development.
- Biological function summary
MTBP participates in the regulation of cell proliferation and survival. It acts as a part of the regulatory complex influencing the p53 pathway which is important for controlling the cell cycle and apoptosis. The interaction with Mdm2 affects the stability and activity of the p53 protein an essential factor in preventing abnormal cell growth.
- Pathways
MTBP has a significant role in the cell cycle regulation and DNA damage response pathways. It is widely associated with the p53 pathway involving p53 and Mdm2 proteins. The regulation of these pathways is fundamental in maintaining genomic integrity and cellular response following DNA damage.
- Associated diseases and disorders
MTBP's misregulation associates with cancer development. Its interaction with Mdm2 and p53 reveals a potential link to tumorigenesis as the imbalance can lead to uncontrollable cell growth. Abnormal activity or expression of MTBP also shows association with glioblastoma an aggressive brain cancer due to its regulatory effects on cell cycle and survival pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTBP antibody (ab115529)
ab115529 at 10ug/ml staining MTBP in Formalin-fixed, Paraffin-embedded Human thymus tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTBP antibody (ab115529)
ab115529 at 10ug/ml staining MTBP in Formalin-fixed, Paraffin-embedded Human tonsil tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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