Name: Anti-MTCO1 antibody [1D6E1A8]
SKU: ab14705
Rating: 5 (43 reviews)

Anti-MTCO1 antibody [1D6E1A8] is a mouse monoclonal antibody used for detecting mitochondrial cytochrome c oxidase subunit 1 (MTCO1) in immunocytochemistry/immunofluorescence (ICC/IF), western blotting (WB), immunohistochemistry (IHC), and flow cytometry (Flow Cyt). Suitable for human, mouse, and rat samples.

- Cited in over 520 publications
- Affinity purification using Protein A
- Kappa light chain type isotype
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (AB14705), expandable thumbnail

Publications

Key facts

Isotype: IgG2a
Host species: Mouse
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC	Flow Cyt	WB	IHC-P
Human	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Tested	Tested
Rat	Tested	Tested	Tested	Tested
Caenorhabditis elegans	Predicted	Predicted	Predicted	Predicted
Cat	Predicted	Predicted	Predicted	Predicted
Chinese hamster	Predicted	Predicted	Predicted	Predicted
Common marmoset	Predicted	Predicted	Predicted	Predicted
Dog	Predicted	Predicted	Predicted	Predicted
Goat	Predicted	Predicted	Predicted	Predicted
Pig	Predicted	Predicted	Predicted	Predicted
Quail	Predicted	Predicted	Predicted	Predicted
Rhesus monkey	Predicted	Predicted	Predicted	Predicted
Sheep	Predicted	Predicted	Predicted	Predicted
Zebrafish	Predicted	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes -
Species Mouse	Dilution info 1/100	Notes -
Species Rat	Dilution info 1/100	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Goat, Pig, Caenorhabditis elegans, Zebrafish, Quail, Rhesus monkey, Chinese hamster, Common marmoset, Sheep, Cat, Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -
Species Mouse	Dilution info 1/500	Notes -
Species Rat	Dilution info 1/500	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Goat, Pig, Caenorhabditis elegans, Zebrafish, Quail, Rhesus monkey, Chinese hamster, Common marmoset, Sheep, Cat, Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Goat, Pig, Caenorhabditis elegans, Zebrafish, Quail, Rhesus monkey, Chinese hamster, Common marmoset, Sheep, Cat, Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/5000	Notes -
Species Mouse	Dilution info 1/5000	Notes -
Species Rat	Dilution info 1/5000	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Goat, Pig, Caenorhabditis elegans, Zebrafish, Quail, Rhesus monkey, Chinese hamster, Common marmoset, Sheep, Cat, Dog	Dilution info -	Notes -

Associated Products

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Target data

See full target information MT-CO1

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Alternative names

COI, COXI, MTCO1, MT-CO1, Cytochrome c oxidase subunit 1, Cytochrome c oxidase polypeptide I

Recommended products

- Cited in over 520 publications
- Affinity purification using Protein A
- Kappa light chain type isotype
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests

Key facts

Isotype: IgG2a
Host species: Mouse
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: 1D6E1A8
Purification technique: Affinity purification Protein A
Light chain type: kappa
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C

Notes

Anti-MTCO1 antibody [1D6E1A8] (ab14705) is a House Mouse recombinant monoclonal antibody and is validated for use in Flow Cyt, ICC, IHC-P, WB in human, recombinant fragment samples.

Anti-MTCO1 antibody [1D6E1A8] (ab14705) has been cited over 521 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality manufacturing and validation processes ensure Anti-MTCO1 antibody [1D6E1A8] (ab14705) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-MTCO1 antibody [1D6E1A8] (ab14705) has 40 independent reviews from customers.

Anti-MTCO1 antibody [1D6E1A8] (ab14705) specifically detects MTCO1 (UniProt ID: P00395; Molecular weight: 57kDa) and is sold in 100 ug and 1 mg selling sizes.

Antibody clone 1D6E1A8 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647 (Alexa Fluor® 488 Anti-MTCO1 antibody [1D6E1A8] ab154477, ab1986).

This product has switched from a hybridoma to recombinant production method on 24th May 2023.

Western blot protocol advice:
For best results with this antibody in Western blot, do not boil samples before loading onto the gel. Boiling of the sample will cause a loss of signal.

Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.

This antibody clone [1D6E1A8] is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MTCO1 also known as COX1 or MT-CO1 is an important component of the mitochondrial respiratory chain’s complex IV commonly called cytochrome c oxidase. This target is encoded by mitochondrial DNA and contributes to the complex's catalytic core. It is a transmembrane protein with a noted molecular weight of approximately 57 kDa. MTCO1 is predominantly expressed in tissues with high energy demand such as cardiac and skeletal muscles due to their reliance on efficient oxidative phosphorylation.

Biological function summary

MTCO1 is vital for the final step of the electron transport chain catalyzing the transfer of electrons from cytochrome c to oxygen. This process facilitates the reduction of oxygen molecules to water. MTCO1 is an integral part of cytochrome c oxidase a multi-subunit enzyme complex important for cellular energy production. Proper function of MTCO1 supports ATP synthesis by maintaining electrochemical gradients across the mitochondrial inner membrane.

Pathways

Electrons transfer through this protein is essential for effective oxidative phosphorylation and maintaining the proton gradient necessary for ATP synthesis. MTCO1 operates in tandem with proteins like COX2 within the electron transport chain to achieve optimal energy conversion and cellular respiration. The pathway interactions of MTCO1 are critical in efficiently powering cellular activities and upholding metabolic functions throughout the body.

Associated diseases and disorders

Mutations or defects in MTCO1 have associations with various mitochondrial diseases such as Leber's Hereditary Optic Neuropathy (LHON) and mitochondrial complex IV deficiency. MTCO1 anomalies may disrupt normal function leading to impaired oxidative phosphorylation and energy deficits in cells. In LHON affected individuals can also demonstrate deficits linked to mutations in other mitochondrial genes like ND1 which further disrupt cellular energy balance and contribute to the clinical manifestations of these mitochondrial disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

Image from Phillips J et al., Sci Rep. 2016 May 16;6:26013. Fig 3b doi: 10.1038/srep26013. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
This image was generated using a previous batch manufactured using hybridoma production method.
250 μm human cerebellar sections from control individuals and a patient with mitochondrial disease underwent passive clearing at 37 °C for 2 or 4 weeks.
The quality of immunofluorescent staining is determined by duration of passive clearing; 2 weeks of passive clearing produced minimal labelling of the white matter in the granule cell layer (NF-H; green; 488 nm and MBP; red, 546 nm) with an absence of labelling of mitochondria (MTCO1 (COXI) (ab14705, 1/100); purple; 647 nm; Extending passive clearing to 4 weeks improved the quality of stain with identifiable Purkinje cells and their axons (NF-H, green; 488 nm) and their myelin sheaths (MBP; red, 546 nm) and mitochondria (MTCO1 (COXI) (ab14705, 1/100); purple; 647 nm.
Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705) at 1/1000 dilution
Lane 1: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
Lane 2: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
Lane 3: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 4: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 5: Human skeletal muscle tissue lysate at 20 µg
Lane 6: Rat heart tissue lysate at 20 µg
Lane 7: Rat liver tissue lysate at 20 µg
Secondary
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Observed band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labelling MTCO1 with ab14705 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Rat colon. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval Solution2) for 20 mins. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling MTCO1 with ab14705 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Human kidney. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval Solution2) for 20 mins. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat glial tumor cells labeling MTCO1 with ab14705 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of Rat glial tumor cells. Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker was used to counterstain tubulin at 1/400 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse neuroblastoma neuroblast cells labeling MTCO1 with ab14705 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of Mouse neuroblastoma neuroblast cells. Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker was used to counterstain tubulin at 1/400 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.
Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
Flow cytometric analysis of Human HeLa cells labelling MTCO1 with ab14705 at 1/500 dilution (Right) compared with an isotype control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at was used as the secondary antibody. Gated on viable cells.
Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-MTCO1 antibody [1D6E1A8] (ab14705) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) non-mitochondrial fraction at 20 µg
Lane 2: HeLa mitochondrial fraction at 20 µg
Lane 3: MCF7(human breast adenocarcinoma epithelial cell) non-mitochondrial fraction at 20 µg
Lane 4: MCF7 mitochondrial fraction at 20 µg
Secondary
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Observed band size: 35 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human cervical adenocarcinoma epithelial cells labeling MTCO1 with ab14705 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution. Confocal image showing cytoplasmic and membranous staining in subsets of Human cervical adenocarcinoma epithelial cells. Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker ab186735 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker was used to counterstain tubulin at 1/400 dilution. The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling MTCO1 with ab14705 at 1/5000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Mouse colon. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval Solution2) for 20 mins. Counterstained with hematoxylin.
Flow Cytometry - Anti-MTCO1 antibody [1D6E1A8] (ab14705)
Flow cytometric analysis of Neuro-2a cells labelling MTCO1 with ab14705 at 1/500 dilution (Right) compared with an isotype control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at was used as the secondary antibody. Gated on viable cells.