AB219824

Anti-MTCO1 antibody [5D11-1C9] - N-terminal

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(1 Publication)

Mouse Monoclonal MTCO1 antibody. N-terminal. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MT-CO1.

View Alternative Names

COI, COXI, MTCO1, MT-CO1, Cytochrome c oxidase subunit 1, Cytochrome c oxidase polypeptide I

2 Images

Supplier Data

Western blot - Anti-MTCO1 antibody [5D11-1C9] - N-terminal (AB219824)

Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.

All lanes:

Western blot - Anti-MTCO1 antibody [5D11-1C9] - N-terminal (ab219824) at 0.1 µg/mL

Lane 1:

Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn) at 10 µg

Lane 2:

Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide at 10 µg

Secondary

All lanes:

HRP-labeled Goat-anti-mouse IgG

Predicted band size: 57 kDa

Observed band size: 40 kDa

true

Supplier Data

Western blot - Anti-MTCO1 antibody [5D11-1C9] - N-terminal (AB219824)

Proteins were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.

All lanes:

Western blot - Anti-MTCO1 antibody [5D11-1C9] - N-terminal (ab219824) at 2 µg/mL

Lane 1:

Whole cell extract of cultured normal control human dermal fibroblasts neonatal (HDFn) at 15 µg

Lane 2:

Whole cell extract HDFn-Rho0 cells depleted of mtDNA by long-term culture in the presence of ethidium bromide at 15 µg

Secondary

All lanes:

HRP-labeled Goat-anti-mouse IgG

Predicted band size: 57 kDa

Observed band size: 40 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5D11-1C9

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human MT-CO1. The exact immunogen used to generate this antibody is proprietary information.

P00395

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

Western blot advice:

Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.

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Properties and storage information

Form

Liquid

Purification notes

Purified from hybridoma cell culture supernatant by Protein L affinity chromatography from fetal bovine serum containing medium (Protein L does not bind bovine IgG).

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 0.87% Sodium chloride, 0.36% HEPES

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MTCO1 also known as COX1 or MT-CO1 is an important component of the mitochondrial respiratory chain’s complex IV commonly called cytochrome c oxidase. This target is encoded by mitochondrial DNA and contributes to the complex's catalytic core. It is a transmembrane protein with a noted molecular weight of approximately 57 kDa. MTCO1 is predominantly expressed in tissues with high energy demand such as cardiac and skeletal muscles due to their reliance on efficient oxidative phosphorylation.

Biological function summary

MTCO1 is vital for the final step of the electron transport chain catalyzing the transfer of electrons from cytochrome c to oxygen. This process facilitates the reduction of oxygen molecules to water. MTCO1 is an integral part of cytochrome c oxidase a multi-subunit enzyme complex important for cellular energy production. Proper function of MTCO1 supports ATP synthesis by maintaining electrochemical gradients across the mitochondrial inner membrane.

Pathways

Electrons transfer through this protein is essential for effective oxidative phosphorylation and maintaining the proton gradient necessary for ATP synthesis. MTCO1 operates in tandem with proteins like COX2 within the electron transport chain to achieve optimal energy conversion and cellular respiration. The pathway interactions of MTCO1 are critical in efficiently powering cellular activities and upholding metabolic functions throughout the body.

Mutations or defects in MTCO1 have associations with various mitochondrial diseases such as Leber's Hereditary Optic Neuropathy (LHON) and mitochondrial complex IV deficiency. MTCO1 anomalies may disrupt normal function leading to impaired oxidative phosphorylation and energy deficits in cells. In LHON affected individuals can also demonstrate deficits linked to mutations in other mitochondrial genes like ND1 which further disrupt cellular energy balance and contribute to the clinical manifestations of these mitochondrial disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

See full target information MT-CO1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 23:e3003207 PubMed40554457

2025

Correction of pathogenic mitochondrial DNA in patient-derived disease models using mitochondrial base editors.

Applications

Unspecified application

Species

Unspecified reactive species

Indi P Joore,Sawsan Shehata,Irena Muffels,Jose Castro-Alpízar,Elena Jiménez-Curiel,Emilia Nagyova,Natacha Levy,Ziqin Tang,Kimberly Smit,Wilbert P Vermeij,Richard Rodenburg,Raymond Schiffelers,Edward E S Nieuwenhuis,Peter M van Hasselt,Sabine A Fuchs,Martijn A J Koppens

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MTCO1 antibody [5D11-1C9] - N-terminal

Western blot - Anti-MTCO1 antibody [5D11-1C9] - N-terminal (AB219824)

Western blot - Anti-MTCO1 antibody [5D11-1C9] - N-terminal (AB219824)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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