Anti-MTCO1 antibody [EPR19628]
- RabMAb
- Recombinant
- 20ul selling size
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(35 Publications)
Rabbit Recombinant Monoclonal MTCO1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 35 publications.
View Alternative Names
COI, COXI, MTCO1, MT-CO1, Cytochrome c oxidase subunit 1, Cytochrome c oxidase polypeptide I
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [EPR19628] (AB203912)
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MTCO1 with ab203912 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm staining on Human cardiac muscle is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-MTCO1 antibody [EPR19628] (AB203912)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling MTCO1with ab203912 at 1/70 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.
Cells were permeabilised with 90% methanol (diluted with 1X PBS).
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [EPR19628] (AB203912)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling MTCO1 with ab203912 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on HeLa cell line.
The nuclear counterstain is DAPI (blue).
COX IV is detected with ab33985 (anti-COX IV mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows : -
-ve control 1 : ab203912 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab33985 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [EPR19628] (AB203912)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling MTCO1 with ab203912 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on HeLa cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows : -
-ve control 1 : ab203912 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [EPR19628] (AB203912)
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling MTCO1 with ab203912 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm staining on Human thyroid cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [EPR19628] (AB203912)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling MTCO1 with ab203912 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on Neuro-2a cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows : -
-ve control 1 : ab203912 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [EPR19628] (AB203912)
Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling MTCO1 with ab203912 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm staining on rat cardiac muscle is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-MTCO1 antibody [EPR19628] (AB203912)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling MTCO1 with ab203912 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on Neuro-2a cell line.
The nuclear counterstain is DAPI (blue).
COX IV is detected with ab33985 (anti-COX IV mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows : -
-ve control 1 : ab203912 at 1/250 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab33985 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTCO1 antibody [EPR19628] (AB203912)
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling MTCO1 with ab203912 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm staining on mouse cardiac muscle is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-MTCO1 antibody [EPR19628] (AB203912)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-MTCO1 antibody [EPR19628] (ab203912) at 1/1000 dilution
Lane 1:
C6 (Rat glial tumor cell line) mitochondria lysate at 10 µg
Lane 2:
SH-SY5Y (Human neuroblastoma cell line from bone marrow) mitochondria lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 57 kDa
Observed band size: 37 kDa
false
Exposure time: 15s
- WB
Supplier Data
Western blot - Anti-MTCO1 antibody [EPR19628] (AB203912)
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : Lane1 : 3 minutes; Lane 2 : 30seconds; Lane 3 and 4 : 10seconds; Lane 5 : 3 minutes; Lane 6 : 1 minute.
The molecular weight observed is consistent with what has been described in the literature (PMID : 23125210 & 22426402).
All lanes:
Western blot - Anti-MTCO1 antibody [EPR19628] (ab203912) at 1/2000 dilution
Lane 1:
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2:
HeLa (Human epithelial cell line from cervix adenocarcinoma) mitochondria lysate at 20 µg
Lane 3:
Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 20 µg
Lane 4:
Neuro-2a (Mouse neuroblastoma cell line) mitochondria lysate at 20 µg
Lane 5:
Mouse liver whole cell lysate at 20 µg
Lane 6:
Mouse liver mitochondria lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 57 kDa
Observed band size: 37 kDa
false
- WB
Supplier Data
Western blot - Anti-MTCO1 antibody [EPR19628] (AB203912)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-MTCO1 antibody [EPR19628] (ab203912) at 1/1000 dilution
All lanes:
Human fetal liver whole cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 37 kDa
false
Exposure time: 1min
- WB
Supplier Data
Western blot - Anti-MTCO1 antibody [EPR19628] (AB203912)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-MTCO1 antibody [EPR19628] (ab203912) at 1/1000 dilution
Lane 1:
C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
Lane 2:
RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 57 kDa
Observed band size: 37 kDa
false
Exposure time: 3min
Related conjugates and formulations (1)
-
Anti-MTCO1 antibody [EPR19628] - BSA and Azide free
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MTCO1 is vital for the final step of the electron transport chain catalyzing the transfer of electrons from cytochrome c to oxygen. This process facilitates the reduction of oxygen molecules to water. MTCO1 is an integral part of cytochrome c oxidase a multi-subunit enzyme complex important for cellular energy production. Proper function of MTCO1 supports ATP synthesis by maintaining electrochemical gradients across the mitochondrial inner membrane.
Pathways
Electrons transfer through this protein is essential for effective oxidative phosphorylation and maintaining the proton gradient necessary for ATP synthesis. MTCO1 operates in tandem with proteins like COX2 within the electron transport chain to achieve optimal energy conversion and cellular respiration. The pathway interactions of MTCO1 are critical in efficiently powering cellular activities and upholding metabolic functions throughout the body.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (35)
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Journal of nanobiotechnology 23:339 PubMed40340852
2025
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Nucleic acids research 53: PubMed39878211
2025
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Cell reports. Medicine 5:101840 PubMed39626672
2024
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Cancer discovery 15:401-426 PubMed39476412
2024
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International journal of nanomedicine 19:9799-9819 PubMed39345912
2024
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Journal of cachexia, sarcopenia and muscle 15:2104-2117 PubMed39187977
2024
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Cell discovery 10:11 PubMed38291322
2024
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American journal of physiology. Renal physiology 326:F326-F337 PubMed38205542
2024
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Antioxidants (Basel, Switzerland) 12: PubMed38136199
2023
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Lipids in health and disease 22:189 PubMed37932729
2023
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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