AB110258

Anti-MTCO2 antibody [12C4F12]

Name: Anti-MTCO2 antibody [12C4F12] (ab110258) | Abcam
Brand: Abcam Limited
SKU: AB110258
Price: 430 USD
Availability: InStock
Rating: 5 (18 reviews)

(18 Reviews)

(276 Publications)

Anti-MTCO2 antibody [12C4F12] (ab110258) is a mouse monoclonal antibody detecting MTCO2 in Western Blot, Flow Cytometry, ICC/IF. Suitable for Human.

- Over 230 publications

View Alternative Names

COII, COX2, COXII, MTCO2, MT-CO2, Cytochrome c oxidase subunit 2, Cytochrome c oxidase polypeptide II

3 Images

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Western blot - Anti-MTCO2 antibody [12C4F12] (AB110258)

All lanes:

Western blot - Anti-MTCO2 antibody [12C4F12] (ab110258) at 1 µg/mL

All lanes:

Mitochondrial lysate from human heart tissue at 5 µg

Predicted band size: 25 kDa

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Flow Cytometry - Anti-MTCO2 antibody [12C4F12] (AB110258)

Flow Cyt

Unknown

Flow Cytometry - Anti-MTCO2 antibody [12C4F12] (AB110258)

ab110258, at 1 µg/ml, staining Cytochrome C oxidase subunit II in HeLa cells by Flow Cytometry (Blue). An isotype control antibody, at 1 µg/ml, staining in HeLa cells (Red).

Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [12C4F12] (AB110258)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [12C4F12] (AB110258)

Immunocytochemistry/Immunofluorescence analysis of human embryonic lung derived fibroblasts (MRC5) labelling Cytochrome C oxidase subunit II with ab110258 at 5 μg/ml. An Alexa Fluor^® 488-conjugated goat anti-mouse IgG2a isotype specific secondary antibody was used at 2 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

12C4F12

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-10 µg/mL", "ICCIF-species-notes": "(heat-induced antigen-retrieval improves signal)" } } }

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Product details

What is this antibody validated in?
Anti-MTCO2 antibody [12C4F12] (ab110258) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of MTCO2?
Anti-MTCO2 [12C4F12] (ab110258) specifically detects a band for MTCO2 (UniProt: P00403) at a molecular weight of 26kDa.

Trusted by the scientific community
Anti-MTCO2 [12C4F12] (ab110258) was first used in a scientific publication in 2011 and has been cited over 230 times in peer-reviewed journals.

Reviewed by scientists
Anti-MTCO2 [12C4F12] (ab110258) has over 10 independent reviews from customers.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

ab110258 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110258 was judged near homogeneity by SDS-PAGE.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'MTCO2' also known as 'mt-co2' or 'mtco2e' is a mitochondrial gene that encodes for a component of the cytochrome c oxidase complex referred to as Complex IV in the electron transport chain. The protein plays a mechanical role in facilitating electron transfer within mitochondria an essential process in cellular respiration. MTCO2 is predominantly expressed in tissues with high energy demands such as muscle and neurons. The known mass of the MTCO2 protein is approximately 25 kDa. It sits in the mitochondrial inner membrane where it contributes to creating the proton gradient driving ATP synthesis.

Biological function summary

MTCO2 (or cytochrome c oxidase subunit II) serves as an important player in aerobic respiration. It is part of the cytochrome c oxidase complex which forms the last enzyme complex of the electron transport chain. As part of this complex MTCO2 facilitates the transfer of electrons from cytochrome c to oxygen resulting in the reduction of oxygen to water. This electron transfer is paired with proton translocation across the mitochondrial membrane which is critical for ATP production.

Pathways

MTCO2 contributes significantly to the oxidative phosphorylation pathway which is essential for ATP production in eukaryotic cells. It directly interacts with other components of the mitochondrial electron transport chain like cytochrome c and NADH dehydrogenase which are critical for maintaining the flow of electrons and the integrity of the energy production process. Another pathway it is part of is the apoptosis pathway regulated by non-lethal stress conditions where controlled release of cytochrome c can trigger programmed cell death.

MTCO2 mutations and dysfunctions have been linked with mitochondrial disorders especially those affecting energy-demanding tissues leading to conditions such as mitochondrial myopathy and Leber's hereditary optic neuropathy. These disorders result from compromised oxidative phosphorylation leading to inadequate energy supply. The dysfunction of cytochrome c oxidase which contains the MTCO2 subunit is a central aspect of these diseases often tying this protein to other complexes within the electron transport chain that also underpin mitochondrial diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

See full target information MT-CO2

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Publications (276)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 33:2421-2434 PubMed40918465

2025

Pyrimethamine Inhibits Human Ovarian Cancer by Triggering Lethal Mitophagy via Activating the p38/JNK/ERK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lingjuan Linghu,Hongying Zhou,Gang Zheng,Tao Yi

Nature cell biology 27:1272-1287 PubMed40715440

2025

Reconstitution of BNIP3/NIX-mitophagy initiation reveals hierarchical flexibility of the autophagy machinery.

Applications

Unspecified application

Species

Unspecified reactive species

Elias Adriaenssens,Stefan Schaar,Annan S I Cook,Jan F M Stuke,Justyna Sawa-Makarska,Thanh Ngoc Nguyen,Xuefeng Ren,Martina Schuschnig,Julia Romanov,Grace Khuu,Louise Uoselis,Michael Lazarou,Gerhard Hummer,James H Hurley,Sascha Martens

Cancer cell international 25:272 PubMed40684193

2025

The DRP1 receptor FIS1 is critical to the expansion of triple-negative breast cancer tumor-initiating cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tetiana Katrii,Tanya Freywald,Malkon G Estrada,Amr El Zawily,Behzad Toosi,Frederick S Vizeacoumar,Franco J Vizeacoumar,Andrew Freywald,Scot C Leary

FASEB bioAdvances 7:e70030 PubMed40641845

2025

PINK1 Loss of Function Selectively Alters the Mitochondrial-Derived Vesicle Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte L Collier,Colleen Ruedi,Naomi J Thorne,David A Tumbarello

International journal of biological sciences 21:4252-4269 PubMed40612683

2025

Coupling of glucose metabolism with mitophagy via O-GlcNAcylation of PINK1.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwei Xu,Xiangzheng Gao,Dade Rong,Jingyao Wang,Liangliang Gao,Mingzhu Tang,Yiguan Chen,Yichi Zhang,Liming Xie,Liming Wang,Guang Lu,Jia-Hong Lu,Wei Liu,Han-Ming Shen

Organogenesis 21:2519649 PubMed40570323

2025

SS-31 Targets NOS2 to Enhance Osteogenic Differentiation in Aged BMSCs by Restoring Mitochondrial Function.

Applications

Unspecified application

Species

Unspecified reactive species

Sen Duan,Qindong Zhang,Jinqiang Zhu,Jiaming Wang

Journal of translational medicine 23:542 PubMed40369632

2025

Mitochondrial dysfunction fuels drug resistance in adult T-cell acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Guo,Ekaterina Bourova-Flin,Sophie Rousseaux,Florent Chuffart,Lijun Peng,Duohui Jing,Jian-Qing Mi,Saadi Khochbin,Jin Wang

Molecules (Basel, Switzerland) 30: PubMed40333775

2025

Modulation of Tumor Metabolism in Acute Leukemia by Plant-Derived Polymolecular Drugs and Their Effects on Mitochondrial Function.

Applications

Unspecified application

Species

Unspecified reactive species

Cindy Arévalo,Carolina Carlosama,Laura Rojas,Mónica P Cala,Marie-Paule Hamon,Bertrand Friguet,Alfonso Barreto,Susana Fiorentino

Scientific reports 15:13811 PubMed40259011

2025

Human RCC1L is involved in the maintenance of mitochondrial nucleoids and mtDNA.

Applications

Unspecified application

Species

Unspecified reactive species

Emi Matsumoto,Taeko Sasaki,Tetsuya Higashiyama,Narie Sasaki

The Journal of biological chemistry 301:108433 PubMed40120684

2025

HS remodels mitochondrial ultrastructure and destabilizes respiratory supercomplexes.

Applications

Unspecified application

Species

Unspecified reactive species

David A Hanna,Brandon Chen,Yatrik M Shah,Oleh Khalimonchuk,Brian Cunniff,Ruma Banerjee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MTCO2 antibody [12C4F12]

Western blot - Anti-MTCO2 antibody [12C4F12] (AB110258)

Flow Cytometry - Anti-MTCO2 antibody [12C4F12] (AB110258)

Immunocytochemistry/ Immunofluorescence - Anti-MTCO2 antibody [12C4F12] (AB110258)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (276)

Product promise